Identification of the biological significance of small chemicals for morphogenesis

• Project/Area Number: 24370092
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: UENO Naoto 基礎生物学研究所, 形態形成研究部門, 教授 (40221105)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Makoto 基礎生物学研究所, 形態形成研究部門, 助教 (10533193)
• Co-Investigator(Renkei-kenkyūsha): NAGAI Takeharu 大阪大学, 産業科学研究所, 教授 (20311350)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2012: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: 胚葉形成 / 原腸形成 / 形態形成 / 低分子化合物 / 細胞応答 / カルシウム / ATP

Outline of Final Research Achievements

We conducted a research to unveil the importance of small chemicals such as calcium and ATP for tissue remodeling and morphogenesis during development by employing live-imaging. We used Xenopus laevis as a model organism that allows to surgically manipulate embryos and fluorescent probes GECO and YC-Nano (FRET）for calcium and ATeam (FRET) for extracellular ATP. As results, we demonstrated that calcium spikes that oscillates are important for cellular morphogenesis such as a@ica constriction, whicle basal-level calcium is essential for cell migration. We also found that extracellular ATP that accumulates at the intercellular spaces (cell-cell boundary) is required for the propagation of calcium waves across tissues.

Research Products

• [Journal Article] Phosphorylation-dependent ubiquitination of paraxial protocadherin (PAPC) controls gastrulation cell movements. (2015)
  • Author(s): Kai, M., Ueno, N. and Kinoshita, N.
  • Journal Title: PLoS One Volume: 10 Issue: 1 Pages: e0115111-e0115111
  • DOI: 10.1371/journal.pone.0115111
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Six1 is a key regulator of the developmental and evolutionary architecture of sensory neurons in craniates (2014)
  • Author(s): H. Yajima, M. Suzuki, H. Ochi, K. Ikeda, S. Sato, K. Yamamura, H. Ogino, N. Ueno and K. Kawakami
  • Journal Title: BMC Biol Volume: 12 Issue: 1 Pages: 40-40
  • DOI: 10.1186/1741-7007-12-40
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Molecular and cellular mechanisms of development underlying congenital disease (2014)
  • Author(s): Masakazu Hashimoto, Hitoshi Morita, Naoto Ueno
  • Journal Title: Congenital anormalies Volume: Vol.54 Issue: 1 Pages: 1-7
  • DOI: 10.1111/cga.12039
  • Peer Reviewed / Open Access
• [Journal Article] Left-right asymmetry is formed in individual cells by intrinsic cell chirality (2014)
  • Author(s): Hatori, R., Ando, T., Sasamura, T., Nakazawa, N., Nakamura, M., Taniguchi, K., Hozumi, S., Kikuta, J., Ishii, M., *Matsuno, K.
  • Journal Title: Mech Dev Volume: 133 Issue: 2 Pages: 482-95
  • DOI: 10.1016/j.ydbio.2013.07.023
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Transgenic Xenopus laevis for live imaging in cell and developmental biology. (2013)
  • Author(s): Takagi, C., Sakamaki, K., Morita, H., Hara, Y., Suzuki, M., Kinoshita, N. and Ueno, N.
  • Journal Title: Development, Growth & Differentiation Volume: 55 Issue: 4 Pages: 422-433
  • DOI: 10.1111/dgd.12042
  • Peer Reviewed
• [Journal Article] Multiple functions of FADD in apoptosis, NF-_KB-related signaling, and heart development in Xenopus embryos. (2012)
  • Author(s): Sakamaki, K., Takagi, C., Kitayama, A., Kurata, T., Yamamoto, T.S., Chiba, K., Kominami, K., Jung, S.K., Okawa, K., Nozaki, M., Kubota, H.Y., Ueno, N.
  • Journal Title: Genes Cells Volume: 17 Pages: 875-896
  • Peer Reviewed
• [Journal Article] Dynamic microtubules at the vegetal cortex predict the embryonic axis in zebrafish. (2012)
  • Author(s): Tran, L.D., Hino, H., Quach, H., Lim, S., Shindo, A., Mimori-Kiyosue, Y., Mione, M., Ueno, N., Winkler, C., Hibi, M., Sampath, K.
  • Journal Title: Development Volume: 139 Pages: 3644-3652
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of cell shape changes that contribute to vertebrate neural tube closure. (2012)
  • Author(s): Suzuki, M., Morita, H. Ueno, N.
  • Journal Title: Dev. Growth Differ. Volume: 54 Pages: 266-276
  • NAID: 10031136900
  • Peer Reviewed
• [Journal Article] Nuclear localization of Prickle2 is required to establish cell polarity during early mouse embryogenesis. (2012)
  • Author(s): Tao, H., Inoue, K., Kiyonari, H., Bassuk, A.G., Axelrod, J.D., Sasaki, H., Aizawa, S., Ueno, N.
  • Journal Title: Dev. Biol. Volume: 364 Pages: 138-148
  • Peer Reviewed
• [Journal Article] Cell movements of the deep layer of non-neural ectoderm underlie complete neural tube closure in Xenopus. (2012)
  • Author(s): Morita, H., Kajiura-Kobayashi, H., Takagi, C., Yamamoto, T.S., Nonaka, S., Ueno, N.
  • Journal Title: Development Volume: 139 Pages: 1417-1426
  • Peer Reviewed