| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Outline of Final Research Achievements |
We conducted a research to unveil the importance of small chemicals such as calcium and ATP for tissue remodeling and morphogenesis during development by employing live-imaging. We used Xenopus laevis as a model organism that allows to surgically manipulate embryos and fluorescent probes GECO and YC-Nano (FRET）for calcium and ATeam (FRET) for extracellular ATP. As results, we demonstrated that calcium spikes that oscillates are important for cellular morphogenesis such as a@ica constriction, whicle basal-level calcium is essential for cell migration. We also found that extracellular ATP that accumulates at the intercellular spaces (cell-cell boundary) is required for the propagation of calcium waves across tissues.
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