| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2014: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
|
|---|
| Outline of Final Research Achievements |
The present study demonstrated that rice beta 1,2-linked xylose residues on N-glycans are critical for plant development and growth under conditions of abiotic stress. Here, our characterization of the rice mutant reduced culm number11(rcn11) showed that RCN11 controls growth of plants exposed to abnormal temperature, salinity and drought conditions. Molecular studies showed that the rcn11 mutation resulted from a 966-bp deletion that caused loss of function of beta 1,2-xylosyltransferase. This enzyme is located in the Golgi apparatus where it catalyzes the transfer of xylose from UDP-xylose to the core beta -linked mannose of N-glycans. RCN11 promoter activity was observed in the basal part of the shoot containing the shoot and axillary meristems and in the base of crown roots. The level of RCN11 expression was regulated by multiple phytohormones and various abiotic stresses suggesting that plant specific N-glycosylation is regulated by multiple signals in rice plants.
|
