Functional analysis of rice beta 1,2-xylosyltransferase in the abiotic stress responses

• Project/Area Number: 24380001
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Breeding science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: KATO KIYOAKI 帯広畜産大学, 畜産学部, 教授 (60271748)
• Co-Investigator(Kenkyū-buntansha): ONISHI Kazumitsu 帯広畜産大学, 畜産学部, 准教授 (50526704)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2014: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000); Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000); Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
• Keywords: 糖鎖 / N-グリカン / β1,2-キシロシルトランスフェラーゼ / 低温ストレス / 熱ストレス / 塩ストレス / 浸透圧ストレス / 低酸素ストレス / Nグルカン / ベータ1,2キシロシルトランスフェラーゼ / 低温 / 熱 / 浸透圧 / 低酸素 / 発芽 / 水ストレス / アブシジン酸 / サイトカイニン / イネ / 植物型糖鎖修飾

Outline of Final Research Achievements

The present study demonstrated that rice beta 1,2-linked xylose residues on N-glycans are critical for plant development and growth under conditions of abiotic stress. Here, our characterization of the rice mutant reduced culm number11(rcn11) showed that RCN11 controls growth of plants exposed to abnormal temperature, salinity and drought conditions. Molecular studies showed that the rcn11 mutation resulted from a 966-bp deletion that caused loss of function of beta 1,2-xylosyltransferase. This enzyme is located in the Golgi apparatus where it catalyzes the transfer of xylose from UDP-xylose to the core beta -linked mannose of N-glycans. RCN11 promoter activity was observed in the basal part of the shoot containing the shoot and axillary meristems and in the base of crown roots. The level of RCN11 expression was regulated by multiple phytohormones and various abiotic stresses suggesting that plant specific N-glycosylation is regulated by multiple signals in rice plants.

Research Products

• [Journal Article] The rice RCN11 gene encodes β1,2-xylosyltransferase and is required for plant responses to abiotic stresses and phytohormones (2015)
  • Author(s): Sho Takano, Shuichi Matsuda, Atsushi Funabiki, Jun-ichi Furukawa, Takaki Yamauchi, Yoshihiko Tokuji, Mikio Nakazono, Yasuro Shinohara, Itsuro Takamure, Kiyoaki Kato
  • Journal Title: Plant Science Volume: 236 Pages: 75-88
  • DOI: 10.1016/j.plantsci.2015.03.022
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The rice REDUCED CULM NUMBER11 gene controls vegetative growth under low-temperature conditions in paddy fields independent of RCN1/OsABCG5 (2013)
  • Author(s): Atsushi Funabiki, Sho Takano, Shuichi Matsuda, Yoshihiko Tokuji, Itsuro Takamure, Kiyoaki Kato
  • Journal Title: Plant Science Volume: 211 Pages: 70-76
  • DOI: 10.1016/j.plantsci.2013.06.011
  • Peer Reviewed
• [Journal Article] Genome-wide analysis and expression profiling of half-size ABC protein subgroup G in rice in response to abiotic stress and phytohormone treatments (2012)
  • Author(s): Matsuda S, A Funabiki, K Furukawa, N Komori, M Koike, Y Tokuji, I Takamure, K Kato
  • Journal Title: Molecular Genetics and Genomics Volume: 287 Issue: 10 Pages: 819-835
  • DOI: 10.1007/s00438-012-0719-3
  • Peer Reviewed
• [Journal Article] The rice RCNl/OsABCG5 mutation is associated with root development in response to nutrient shortage (2012)
  • Author(s): Ureshi A, S Matuda, E Ohashi, K Onishi, I Takamure, K Kato
  • Journal Title: Plant Root Volume: 6 Pages: 28-35
  • Peer Reviewed
• [Presentation] イネのβ１，２－キシロシルトランスフェラーゼ遺伝子は非生物的ストレス下での生育に関わる (2014)
  • Author(s): 髙野 翔・船引厚志・古川潤一・松田修一・篠原康郎・高牟禮逸朗・加藤清明
  • Organizer: 日本育種学会・作物学会北海道談話会
  • Place of Presentation: 酪農学園大学
  • Year and Date: 2014-12-06