Identification of genes responsible for interspecific incompatibility and hybrid embryo breakdown

• Project/Area Number: 24380002
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Breeding science
• Research Institution: Tohoku University
• Principal Investigator: NISHIO Takeshi 東北大学, (連合)農学研究科(研究院), 教授 (30301039)
• Co-Investigator(Kenkyū-buntansha): KITASHIBA Hiroyasu 東北大学, 大学院農学研究科, 准教授 (80431542)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
• Keywords: 種間交雑 / 生殖的隔離 / 不和合性 / 胚発達 / アブラナ

Outline of Final Research Achievements

A QTL for interspecific incompatibility of Brassica rapa to Brassica oleracea pollen was delimited to 3.7-Mb region on chromosome 2, and three genes were found to be candidates for the interspecific-incompatibility gene by nucleotide sequencing of genes in this region. Two QTLs for hybrid embryo breakdown of B. rapa in intergeneric hybridization with Raphanus sativus were detected in repeated investigations, and BrFIEa and BrMSI1a were identified as candidates for the genes participating in hybrid embryo breakdown. Interestingly, these QTLs may also responsible for embryo breakdown in interspecific hybridization with Brassica napus and Brassica nigra.

Research Products

• [Journal Article] The use of species-specific DNA markers for assessing alien chromosome transfer in Brassica rapa and Brassica oleracea monosomic additions of Raphanus sativus. (2014)
  • Author(s): Tonosaki K, Akaba M, Bang SW, Kitashiba H, Kaneko Y, Nishio T
  • Journal Title: Molecular Breeding Volume: 34 Issue: 3 Pages: 1301-1311
  • DOI: 10.1007/s11032-014-0117-0
  • NAID: 120006581095
  • Peer Reviewed
• [Journal Article] Commonalities and differences between Brassica and Arabidopsis self-incompatibility. (2014)
  • Author(s): Yamamoto M, Nishio T
  • Journal Title: Horticulture Research Volume: 1 Issue: 1 Pages: 14054-14054
  • DOI: 10.1038/hortres.2014.54
  • Peer Reviewed / Open Access
• [Journal Article] Self-incompatibility in Brassicaceae crops: lessons for interspecific incompatibility (2014)
  • Author(s): Kitashiba H, Nasrallah JB
  • Journal Title: Breeding Science Volume: 64 Issue: 1 Pages: 23-37
  • DOI: 10.1270/jsbbs.64.23
  • NAID: 130004146419
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed / Open Access
• [Journal Article] Site-specific N-glycosylation of the S-locus receptor kinase and its role in the self-incompatibility response of the Brassicaceae. (2014)
  • Author(s): Yamamoto M, Tantikanjana T, Nishio, T, Nasrallah ME, Nasrallah JB
  • Journal Title: Plant Cell Volume: 26 Issue: 12 Pages: 4749-4762
  • DOI: 10.1105/tpc.114.131987
  • Peer Reviewed
• [Journal Article] Genetic analysis of hybrid seed formation ability of Brassica rapa in intergeneric crossings with Raphanus sativus. (2013)
  • Author(s): Tonosaki K, Michiba K, Bang SW, Kitashiba H, Kaneko Y, Nishio T
  • Journal Title: Theor Appl Genet Volume: 126 Issue: 3 Pages: 837-846
  • DOI: 10.1007/s00122-012-2021-5
  • Peer Reviewed
• [Presentation] Brassica rapaの属間雑種形成能QTL領域内に座乗する遺伝子の解析 (2013)
  • Author(s): 殿崎薫, 北柴大泰, 金子幸雄, 西尾剛
  • Organizer: 日本育種学会
  • Place of Presentation: (東京農業大学)
  • Year and Date: 2013-03-27
• [Presentation] Brassica napuaとの種間雑種形成能に関与するBrassica rapaのBrFIEa及びBrMSI1aの変異 (2013)
  • Author(s): 殿崎薫・北柴大泰・金子幸雄・西尾剛
  • Organizer: 日本育種学会
  • Place of Presentation: 鹿児島大学
• [Book] Self-incompatibility. In “Biotechnology of Crucifers” ed. by Gupta SK (2013)
  • Author(s): Kitashiba H, Nishio T
  • Total Pages: 241
  • Publisher: Springer