Physiological function of the strong-culm and high-yielding genes for developing a superior lodging resistant rice varieties

• Project/Area Number: 24380008
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Crop science/Weed science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: OOKAWA Taiichiro 東京農工大学, (連合)農学研究科(研究院), 准教授 (80213643)
• Co-Investigator(Kenkyū-buntansha): HIRASAWA Tadashi 東京農工大学, 大学院農学研究院, 教授 (30015119)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2012: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
• Keywords: 水稲 / 倒伏抵抗性 / QTL / 強稈遺伝子 / 多収品種 / 強稈 / ピラミディング / 集積系統 / 皮層繊維組織 / 原因遺伝子 / 遺伝子 / リグニン / 細胞分裂

Outline of Final Research Achievements

To improve the lodging resistance in rice using superior allele of strong culm genes in natural vaeients, the QTLs and causal genes for strong culm were identified using the backcross inbred lines (BILs) derived from Chugoku117 with a strong culms and Koshihikari. QTLs for the culm thickness were detected on chromosomes 2 and 3. We isolated responsible gene of SCM3 by positional cloning. SCM3 was narrowed down, and only one candidate gene was found. To evaluate the effect of SCM3 and the another strong culm gene SCM4 on lodging resistance, NIL-SCM3 and NIL-SCM4 were developed. These NILs showed enhanced culm strength and increased spikelet number due to the pleiotropic effects of the gene. These results demonstrate the importance of identifying superior alleles of strong culm genes in natural variants and pyramiding these genes for improving high yielding varieties with a superior lodging resistance.

Research Products

• [Journal Article] Isolation of a Novel Lodging Resistance QTL Gene Involved in Strigolactone Signaling and Its Pyramiding with a QTL Gene Involved in Another Mechanism (2015)
  • Author(s): Kenji Yano, Taiichiro Ookawa, Koichiro Aya, Yusuke Ochiai, Tadashi Hirasawa, Takeshi Ebitani, Takeshi Takarada, Masahiro Yano, Toshio Yamamoto, Shuichi Fukuoka, Jianzhong Wu, Tsuyu Ando, Reynante Lacsamana Ordonio, Ko Hirano and Makoto Matsuoka
  • Journal Title: Molecular Plant Volume: 8 Issue: 2 Pages: 303-314
  • DOI: 10.1016/j.molp.2014.10.009
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Increased lodging resistance in long-culm, low-lignin gh2 rice for improved feed and bioenergy production (2014)
  • Author(s): Taiichiro Ookawa, Kazuya Inoue, Makoto Matsuoka, Takeshi Ebitani, Takeshi Takarada, Toshio Yamamoto, Tadamasa Ueda, Tadashi Yokoyama, Chisato Sugiyama, Satoshi Nakaba, Ryo Funada, Hiroshi Kato, Motoki Kanekatsu, Koki Toyota, Takashi Motobayashi, Mehran Vazirzanjani, Seisyu Tojo, Tadashi Hirasawa
  • Journal Title: Scientic Reports Volume: 4 Issue: 1 Pages: 1038-1038
  • DOI: 10.1038/srep06567
  • NAID: 40020309034
  • Peer Reviewed / Open Access
• [Presentation] 水稲における強稈関連形質の量的形質遺伝子座の特定とその作用機作 (2015)
  • Author(s): 山本一洋、山本敏央、上田忠正、平沢正、大川泰一郎
  • Organizer: 日本作物学会第239 回講演会
  • Place of Presentation: 日本大学（神奈川県藤沢市）
  • Year and Date: 2015-03-27 – 2015-03-28
• [Presentation] 水稲の強稈性に関与する太稈および強稈質関連形質の量的形質遺伝子座解析 －リーフスターとコシヒカリの組換え自殖系統を用いて－ (2015)
  • Author(s): 鈴木浩貴、松木美紗、山本敏央、上田忠正、平沢正、大川泰一郎
  • Organizer: 日本作物学会第239 回講演会
  • Place of Presentation: 日本大学（神奈川県藤沢市）
  • Year and Date: 2015-03-27 – 2015-03-28
• [Presentation] Analysis of QTLs for strong culm traits using reciprocal chromosome segment substitution lines derived from a cross between rice varieties Koshihikari and Takanari (2014)
  • Author(s): Toshiya Kobayashi, Ryo Aoba, Toshio Yamamoto, Toshiyuki Takai, Chisato Sugiyama, Kazuhiro Yamamoto, Tomohiro Hattori, Mehran Vazirzanjani, Tadashi Hirasawa, Taiichiro Ookawa
  • Organizer: The 8th Asian Crop Science Association Conference
  • Place of Presentation: ベトナム国立農業大学（ベトナム、ハノイ市）
  • Year and Date: 2014-09-23 – 2014-09-25
• [Presentation] Estimation of quantitative trait loci associated with lodging resistance using a set of reciprocal chromosome segment substitution lines derived from crosses between rice varieties Koshihikari and Takanari (2014)
  • Author(s): Kazuhiro Yamamoto, Toshio Yamamoto, Chisato Sugiyama, Tadashi Hirasawa, Taiichiro Ookawa
  • Organizer: The 8th Asian Crop Science Association Conference
  • Place of Presentation: ベトナム国立農業大学（ベトナム、ハノイ市）
  • Year and Date: 2014-09-23 – 2014-09-25
• [Presentation] New approach for improving the lodging resistance in rice using superior alleles of strong culm genes in natural variants (2014)
  • Author(s): Taiichiro Ookawa, Toshiya Kobayashi, Takeshi Ebitani, Toshio Yamamoto, Tadashi Hirasawa
  • Organizer: The 8th Asian Crop Science Association Conference
  • Place of Presentation: ベトナム国立農業大学（ベトナム、ハノイ市）
  • Year and Date: 2014-09-23 – 2014-09-25
• [Presentation] コシヒカリ／タカナリ染色体断片置換系統群を用いた水稲の強稈性に関与する量的形質遺伝子座の推定 (2014)
  • Author(s): 青羽遼、山本敏央、平沢正、大川泰一郎
  • Organizer: 日本作物学会第237回講演会
  • Place of Presentation: 千葉大学（千葉県千葉市）
• [Presentation] 水稲品種リーフスターとリーフスター/タカナリ交雑後代における強稈性に関与する稈の構造的特性 (2013)
  • Author(s): 杉山知里
  • Organizer: 日本作物学会
  • Place of Presentation: 明治大学
  • Year and Date: 2013-03-28
• [Presentation] 水稲における倒伏抵抗性に関与する強稈遺伝子座の推定 : コシヒカリとタカナリの染色体断片置換系統および組換固定系統を用いて (2013)
  • Author(s): 岡庭侑香
  • Organizer: 日本作物学会
  • Place of Presentation: 明治大学
  • Year and Date: 2013-03-28
• [Presentation] 水稲の倒伏抵抗性を高める強稈関連形質の遺伝子座の推定―コシヒカリとタカナリの正逆染色体断片置換系統群を用いて― (2013)
  • Author(s): 山本一洋、山本敏央、上田忠正、杉山知里、岡庭侑香、平沢正、大川泰一郎
  • Organizer: 日本作物学会第236 回講演会
  • Place of Presentation: 鹿児島大学（鹿児島県鹿児島市）
• [Presentation] Estimation of the locus for the traits associated with a strong culm, using reciprocal chromosome segment substitution lines derived from the cross between rice varieties, Koshihikari and Takanari (2013)
  • Author(s): Kazuhiro Yamamoto, Toshio Yamamoto, Chisato Sugiyama, Tadashi Hirasawa and Taiichiro Ookawa
  • Organizer: The 7th Rice Genetics Symposium
  • Place of Presentation: Dusit Thani Hotel (Manilla, Philippines)
• [Presentation] 水稲品種コシヒカリを遺伝背景とする強稈関連量的形質遺伝子座の集積 (2013)
  • Author(s): 丸山甲晃、伊山幸秀、蛯谷武志、小林俊也、平沢正、大川泰一郎
  • Organizer: 日本育種学会第124 回講演会
  • Place of Presentation: 鹿児島大学（鹿児島県鹿児島市）
• [Presentation] 水稲品種コシヒカリとタカナリの正逆染色体断片置換系統群を用いた強稈関連形質の遺伝 (2012)
  • Author(s): 山本一洋
  • Organizer: 日本作物学会
  • Place of Presentation: 日本大学
  • Year and Date: 2012-12-07
• [Presentation] タカナリ/コシヒカリ染色体断片置換系統群を用いた水稲の強稈哇関連形質の遺伝了-座の推定 (2012)
  • Author(s): 杉山知里
  • Organizer: 日本作物学会
  • Place of Presentation: 東北大学
  • Year and Date: 2012-09-10