The establishment of the genome research bases and the evaluation of quantitative traits for flower colors in Lilium
| Project/Area Number |
24380014
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | Hokkaido University |
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Principal Investigator |
YAMAGISHI Masumi 北海道大学, (連合)農学研究科(研究院), 准教授 (40210348)
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| Co-Investigator(Kenkyū-buntansha) |
MASUTA Chikara 北海道大学, 大学院農学研究院, 教授 (60281854)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Keywords | ウイルスベクター / アントシアニン / VIGS / 花の模様 / 転写因子 / R2R3-MYB / DFR / RNA-seq / カロテノイド / マイクロサテライト / ウイルスべクター / 花弁の模様 |
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| Outline of Final Research Achievements |
Lilies (Lilium spp.) are one of the most important floricultural crops worldwide. The lack of reverse genetics tools and gene-sequence information has made it challenging to evaluate the genetic backgrounds of useful traits such as flower colours. In this study, virus-induced gene silencing technique was established using Cucumber mosaic virus as a viral vector, and the sequences expressed in flower tepals were categorized using an RNA-seq technique. In addition, useful genes that determined flower colours in lilies were clarified: LhMYB12-Lat determined splatter-type flower spots in Asiatic hybrid lilies, LhMYB15 was responsible for the light-responsive bud-blush phenotype in Lilium regale, and two independent genes respectively generated white flowers in Lilium speciosum.
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Report
(4 results)
Research Products
(18 results)
