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The establishment of the genome research bases and the evaluation of quantitative traits for flower colors in Lilium

Research Project

Project/Area Number 24380014
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Horticulture/Landscape architecture
Research InstitutionHokkaido University

Principal Investigator

YAMAGISHI Masumi  北海道大学, (連合)農学研究科(研究院), 准教授 (40210348)

Co-Investigator(Kenkyū-buntansha) MASUTA Chikara  北海道大学, 大学院農学研究院, 教授 (60281854)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywordsウイルスベクター / アントシアニン / VIGS / 花の模様 / 転写因子 / R2R3-MYB / DFR / RNA-seq / カロテノイド / マイクロサテライト / ウイルスべクター / 花弁の模様
Outline of Final Research Achievements

Lilies (Lilium spp.) are one of the most important floricultural crops worldwide. The lack of reverse genetics tools and gene-sequence information has made it challenging to evaluate the genetic backgrounds of useful traits such as flower colours. In this study, virus-induced gene silencing technique was established using Cucumber mosaic virus as a viral vector, and the sequences expressed in flower tepals were categorized using an RNA-seq technique. In addition, useful genes that determined flower colours in lilies were clarified: LhMYB12-Lat determined splatter-type flower spots in Asiatic hybrid lilies, LhMYB15 was responsible for the light-responsive bud-blush phenotype in Lilium regale, and two independent genes respectively generated white flowers in Lilium speciosum.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (18 results)

All 2015 2014 2013 2012

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) Book (1 results)

  • [Journal Article] The origin of the LhMYB12 gene, which regulates anthocyanin pigmentation of tepals, in Oriental and Asiatic hybrid lilies (Lilium spp.)2014

    • Author(s)
      Masumi Yamagishi, Hideo Ihara, Katsuro Arakawa, Shinya Toda, Kazuma Suzuki
    • Journal Title

      Scientia Horticulturae

      Volume: 174 Pages: 119-125

    • DOI

      10.1016/j.scienta.2014.05.017

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The novel allele of the LhMYB12 gene is involved in splatter-type spot formation on the flower tepals of Asiatic hybrid lilies (Lilium spp.)2014

    • Author(s)
      Masumi Yamagishi, Shinya Toda, and Keisuke Tasaki
    • Journal Title

      New Phytlogist

      Volume: 201 Issue: 3 Pages: 1009-1020

    • DOI

      10.1111/nph.12572

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A review of target gene specificity of flavonoid R2R3-MYB transcription factors and a discussion of factors contributing to the target gene selectivity2013

    • Author(s)
      Yunsong Lai, Huanxiu Li, and Masumi Yamagishi
    • Journal Title

      Frontiers in Biology

      Volume: 8 Issue: 6 Pages: 577-598

    • DOI

      10.1007/s11515-013-1281-z

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] How genes paint lily flowers: regulation of colouration and pigmentation patterning2013

    • Author(s)
      Masumi Yamagishi
    • Journal Title

      Scientia Horticulturae

      Volume: 163 Pages: 27-37

    • DOI

      10.1016/j.scienta.2013.07.024

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Morphology and heredity of tepal spots in Asiatic and Oriental hybrid lilies (Lilium spp.)2013

    • Author(s)
      Masumi Yamagishi and Koichi Akagi
    • Journal Title

      Euphytica

      Volume: 194 Issue: 3 Pages: 325-334

    • DOI

      10.1007/s10681-013-0937-8

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The transcription factor LhMYB12 determines anthocyanin pigmentation in the tepals of Asiatic hybrid lilies(Lilium spp.) and regulates pigment quantity2012

    • Author(s)
      Masumi Yamagishi, Yusuke Yoshida and Masayoshi Nakayama
    • Journal Title

      Molecular Breeding

      Volume: (in press) Issue: 2 Pages: 913-925

    • DOI

      10.1007/s11032-011-9675-6

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reduced transcription of a LEAFY-like gene in Alstroemeria sp. cultivar Green Coral that cannot develop floral meristems2012

    • Author(s)
      Masayo Hirai, Masumi Yamagishi and Akira Kanno
    • Journal Title

      Plant Science

      Volume: 185-186 Pages: 298-308

    • DOI

      10.1016/j.plantsci.2011.12.017

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pigment accumulation and transcription of LhMYB12 and anthocyanin biosynthesis genes during flower development in the Asiatic hybrid lily (Lilium Spp.)2012

    • Author(s)
      Yun-Song Lai, Yoshihiro Shimoyamada, Masayoshi Nakayama and Masumi Yamagishi
    • Journal Title

      Plant Science

      Volume: 193-194 Pages: 136-147

    • DOI

      10.1016/j.plantsci.2012.05.013

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] スカシユリ花被片における突起斑点ではLhbHLH2は柔細胞でも発現している2015

    • Author(s)
      坂間博子・山岸真澄・堀内和奈・増田清
    • Organizer
      園芸学会平成27年度春期大会研究発表
    • Place of Presentation
      千葉大学(千葉市)
    • Year and Date
      2015-03-28 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] RNA-Seqを用いたアジアティックハイブリッドユリの花被片着色におけるトランスクリプトーム解析2015

    • Author(s)
      鈴木一眞・松山光平・鈴木智大・中塚貴司・道羅英夫・山岸真澄
    • Organizer
      園芸学会平成27年度春期大会研究発表
    • Place of Presentation
      千葉大学(千葉市)
    • Year and Date
      2015-03-28 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 新規R2R3-MYBがリーガルユリの葉や花におけるアントシアニン生合成を制御している2014

    • Author(s)
      山岸真澄
    • Organizer
      園芸学会平成26年度秋期大会研究発表
    • Place of Presentation
      佐賀大学(佐賀市)
    • Year and Date
      2014-09-27 – 2014-09-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] コオニユリにおけるVIGSシステムの開発2014

    • Author(s)
      田﨑啓介・寺田寛之・増田税・山岸真澄
    • Organizer
      第32回日本植物細胞分子生物学会(盛岡)大会・シンポジウム
    • Place of Presentation
      アイーナ(いわて県民情報交流センター)(盛岡市)
    • Year and Date
      2014-08-21 – 2014-08-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] VIGSシステムによるコオニユリの葉および花の色の改変2014

    • Author(s)
      田﨑啓介・寺田寛之・増田税・山岸真澄
    • Organizer
      園芸学会
    • Place of Presentation
      筑波大学(つくば市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 3倍体オニユリと2倍体コオニユリの交雑に由来するF1個体の諸形質と染色体数2014

    • Author(s)
      鈴木雄大・山岸真澄
    • Organizer
      園芸学会
    • Place of Presentation
      筑波大学(つくば市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 2つの異なる変異がシロカノコユリの成立に関わっている2013

    • Author(s)
      鈴木一眞・山岸真澄・田﨑啓介
    • Organizer
      日本育種学会
    • Place of Presentation
      鹿児島大学(鹿児島市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] オリエンタルハイブリッドユリにおける白色花被片の起源2013

    • Author(s)
      鈴木一眞・田﨑啓介・山岸真澄
    • Organizer
      園芸学会
    • Place of Presentation
      岩手大学(盛岡市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ユリ属におけるアントシアニン生合成のMYBによる制御2012

    • Author(s)
      山岸真澄
    • Organizer
      園芸学会
    • Place of Presentation
      福井県立大学(福井県松岡町)(招待講演)
    • Related Report
      2012 Annual Research Report
  • [Book] 4.3 Floriculture2015

    • Author(s)
      Masumi Yamagishi
    • Total Pages
      4
    • Publisher
      Research Faculty of Agriculture, Hokkaido University
    • Related Report
      2014 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-07-29  

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