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Breeding of new pear cultivars by constructing efficient breeding system using large scale genome information and F2 self population

Research Project

Project/Area Number 24380022
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Horticulture/Landscape architecture
Research InstitutionKyoto Prefectural University (2016)
Tottori University (2012-2015)

Principal Investigator

ITAI Akihiro  京都府立大学, 生命環境科学研究科(系), 教授 (10252876)

Co-Investigator(Kenkyū-buntansha) 児玉 基一朗 (児玉 基一郎)  鳥取大学, 農学部, 教授 (00183343)
村山 秀樹  山形大学, 農学部, 教授 (40230015)
山本 俊哉  国立研究開発法人農業・食品産業技術総合研究機構, その他部局等, その他 (60355360)
Project Period (FY) 2012-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywordsナシ / 連鎖地図 / 病害罹病性 / QTL解析 / QTL解析 / 病害抵抗性育種 / 単為結実性 / マーカー育種 / 果実形質 / 病害抵抗性 / SSR / 単為結果 / 黒星病 / ショ糖蓄積 / 次世代シークエンサー / SNP / SSR
Outline of Final Research Achievements

Using an F2 population from a self cross of F1 between European pear ‘ Max Red Bartlett’ and Japanese pear ‘Osa Nijisseiki’, we constructed integrated genetic linkage map consisted of SSR markers derived from largescale information of apple, European pear and Japanese pear. We also performed quantitative trait locus (QTL) analysis of brown spot disease susceptibility and resulted in making SSR markers linked to its susceptibility. The molecular mechanism of parthenocarpy has not understood yet. Here, we have searched genes related to parthenocarpic fruit in pear by RNA-seq analysis. Expression of several trans factors genes showed strongly correlated to the development of parthenocarpic fruit. These candidate genes were selected for further transgenic experiment to clarify the role of parthenocarpy.

Report

(6 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (42 results)

All 2016 2015 2014 2013 2012 Other

All Journal Article (19 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 19 results,  Open Access: 2 results) Presentation (21 results) (of which Invited: 2 results) Book (2 results)

  • [Journal Article] alidation of molecular markers associated with fruit ripening day of Japanese pear (Pyrus pyrifolia Nakai) using variance components.2016

    • Author(s)
      Nishio, S.
    • Journal Title

      Scientia Horticulturae

      Volume: 199 Pages: 9-14

    • DOI

      10.1016/j.scienta.2015.12.032

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genomics of pear and other Rosaceae fruit trees2016

    • Author(s)
      Yamamoto, T.
    • Journal Title

      Breeding Science

      Volume: 66 Issue: 1 Pages: 148-159

    • DOI

      10.1270/jsbbs.66.148

    • NAID

      130005141825

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fine mapping of the gene for susceptibility to black spot disease in Japanese pear (<i>Pyrus pyrifolia</i> Nakai)2016

    • Author(s)
      Terakami, S.
    • Journal Title

      Breeding Science

      Volume: 66 Issue: 2 Pages: 271-280

    • DOI

      10.1270/jsbbs.66.271

    • NAID

      130005144053

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of the expression of chlorophyll-degradation-related genes during ripening between stay-green and yellow-pear cultivars2015

    • Author(s)
      Nongluk Charoenchongsuk, Kazuo Ikeda, Akihiro Itai, Akira Oikawa, Hideki Murayama
    • Journal Title

      Scientia Horticulturae

      Volume: 181 Pages: 89-94

    • DOI

      10.1016/j.scienta.2014.10.005

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic mapping and pyramiding of two new pear scab resistance QTLs2015

    • Author(s)
      Perchepied, L.
    • Journal Title

      Molecular Breeding

      Volume: 35 Issue: 10 Pages: 197-197

    • DOI

      10.1007/s11032-015-0391-5

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Transcriptome-based single nucleotide polymorphism markers for genome mapping in Japanese pear (Pyrus pyrifolia Nakai)2015

    • Author(s)
      Terakami S.
    • Journal Title

      Tree Genetics & Genomes

      Volume: 10 Issue: 4 Pages: 853-863

    • DOI

      10.1007/s11295-014-0726-0

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibitory Factors That Affect the Ripening of Pear Fruit on the Tree2015

    • Author(s)
      Murayama H.
    • Journal Title

      The Horticulture Journal

      Volume: 84 Issue: 1 Pages: 14-20

    • DOI

      10.2503/hortj.MI-015

    • NAID

      130005065753

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of QTLs controlling harvest time and fruit skin color in Japanese pear (<i>Pyrus pyrifolia</i> Nakai)2014

    • Author(s)
      Yamamoto T.
    • Journal Title

      Breeding Science

      Volume: 64 Issue: 4 Pages: 351-361

    • DOI

      10.1270/jsbbs.64.351

    • NAID

      130004888875

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microarray analysis of gene expression patterns during fruit development in European pear (Pyrus communis).2013

    • Author(s)
      奈島 賢児
    • Journal Title

      Scientia Horticulturae

      Volume: 164 Pages: 466-473

    • DOI

      10.1016/j.scienta.2013.09.054

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptome Analysis of Giant Pear Fruit with Fruit-specific DNA Reduplication on a Mutant Branch2013

    • Author(s)
      Nashima K
    • Journal Title

      Journal of the Japanese Society for Horticultural Science

      Volume: 82 Pages: 301-311

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA markers developed from genome sequencing analysis in Japanese pear (Pyrus pyrifolia).2013

    • Author(s)
      Yamamoto T.
    • Journal Title

      Acta Horticulturae

      Volume: 976 Pages: 477-483

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Agrobacterium-mediated genetic transformation using cotyledons in Japanese pear (Pyrus pyrifolia).2013

    • Author(s)
      Nakajima I.
    • Journal Title

      Breeding Science

      Volume: 66 Pages: 275-283

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genomic prediction of trait segregation in a progeny population: a case study of Japanese pear (Pyrus pyrifolia).2013

    • Author(s)
      Iwata H.
    • Journal Title

      BMC Genetics

      Volume: 14 Issue: 1 Pages: 81-81

    • DOI

      10.1186/1471-2156-14-81

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Potential assessment of genome-wide association study and genomic selection in Japanese pear Pyrus pyri瓦五a.2013

    • Author(s)
      Iwata, H.
    • Journal Title

      Breeding Science

      Volume: 63 Pages: 125-140

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptome analysis of Pyrus pyrifolia leaf buds during transition from endodormancy to ecodormancy2012

    • Author(s)
      Nishitani, C.
    • Journal Title

      Scientia Horticulturae

      Volume: 147 Pages: 49-55

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analyses of early rough bark phenotype found in seedlings of a cross between the European pear'Bartlett' and the Chinese pear Yali2012

    • Author(s)
      Ogata, A.
    • Journal Title

      Scientia Horticulturae

      Volume: 148 Pages: 1-8

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 4塩基,5塩基モチーフSSRマーカーのニホンナシ品種識別への利用2012

    • Author(s)
      山本俊哉
    • Journal Title

      DNA多型

      Volume: 20 Pages: 58-61

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Complete sequence of the chloroplast genome from pear (Pyrus pyrifolia) : genome structure and comparative analysis.2012

    • Author(s)
      Terakami, S.
    • Journal Title

      Tree Genetics & Genomes

      Volume: 8 Pages: 841-854

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Redefinition of the map position and validation of a major quantitative trait locus for fire blight resistance of the pear cultivar 'Harrow Sweet' (Pyrus communis L.).2012

    • Author(s)
      Pierre-Marie, F.
    • Journal Title

      Plant Breeding

      Volume: 131 Issue: 5 Pages: 656-664

    • DOI

      10.1111/j.1439-0523.2012.02000.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] ナシ果実成熟期の ACC 合成酵素遺伝子 (PpACS1) のプロモーター領域のメチル化解析2015

    • Author(s)
      板井章浩
    • Organizer
      植物化学調節学会
    • Place of Presentation
      東京大学
    • Year and Date
      2015-10-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] ナシブラウンスポット病罹病性遺伝子座のマッピング2015

    • Author(s)
      友松康一
    • Organizer
      園芸学会
    • Place of Presentation
      徳島大学
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] セイヨウナシACC合成酵素遺伝子(PcACS1)の低温誘導性発現メカニズムの解析2015

    • Author(s)
      藤井美希
    • Organizer
      園芸学会
    • Place of Presentation
      徳島大学
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] ニホンナシの収穫期に関連するDNAマーカーの検証2015

    • Author(s)
      西尾聡悟
    • Organizer
      園芸学会
    • Place of Presentation
      千葉大学
    • Year and Date
      2015-03-28 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] ニホンナシ品種の糖組成およびSSR多型による遺伝的類縁関係の評価2015

    • Author(s)
      小仁所邦彦
    • Organizer
      園芸学会
    • Place of Presentation
      千葉大学
    • Year and Date
      2015-03-28 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] ナシACC合成酵素遺伝子(PpACS1)のプロモーター領域におけるメチル化解析および核タンパク質との 相互作用解析2015

    • Author(s)
      藤井美希
    • Organizer
      園芸学会
    • Place of Presentation
      千葉大学
    • Year and Date
      2015-03-28 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] ナシACC合成酵素(PpACS1)のプロモーター領域のメチル化解析2014

    • Author(s)
      藤井美希
    • Organizer
      園芸学会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2014-09-27 – 2014-09-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 最適な両親組合せを決定するゲノムワイド予測-ニホンナシでの検証-2014

    • Author(s)
      山本俊哉
    • Organizer
      園芸学会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2014-09-27 – 2014-09-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Transcriptome analysis reveals the role of plant hormones in pear fruit set.2014

    • Author(s)
      Itai A.
    • Organizer
      XII International Pear Symposium
    • Place of Presentation
      Leuven University, Belgium
    • Year and Date
      2014-07-14 – 2014-07-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Starch degradation after harvest in ‘La France’ pears.2014

    • Author(s)
      Murayama H.
    • Organizer
      XII International Pear Symposium
    • Place of Presentation
      Leuven University, Belgium
    • Year and Date
      2014-07-14 – 2014-07-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 次世代シーケンサーを用いたトランスクリプトーム解析によるナシ着果制御機構の解明2014

    • Author(s)
      森下恭行
    • Organizer
      園芸学会
    • Place of Presentation
      筑波大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] セイヨウナシ‘ラ・フランス’の果肉における成長ステージ別マイクロアレイ解析2013

    • Author(s)
      奈島賢児
    • Organizer
      園芸学会
    • Place of Presentation
      岩手大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] ニホンナシ果実成熟期に発現変動するbHLH遺伝子の研究2013

    • Author(s)
      滋田徳美
    • Organizer
      園芸学会
    • Place of Presentation
      岩手大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] ニホンナシ黒斑病罹病性遺伝子近傍領域の塩基配列決定2013

    • Author(s)
      寺上伸吾
    • Organizer
      園芸学会
    • Place of Presentation
      岩手大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 気候変動に対応した果樹育種2013

    • Author(s)
      山本俊哉
    • Organizer
      育種学会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] セイヨウナシミクルマス・ネリスにおける樹上成熟と追熟の相2012

    • Author(s)
      小飯塚紗希
    • Organizer
      園芸学会
    • Place of Presentation
      福井県立大学
    • Year and Date
      2012-09-23
    • Related Report
      2012 Annual Research Report
  • [Presentation] マイクロアレイ解析によるGA誘導性ナシ単為結果関連遺伝子の探索2012

    • Author(s)
      板井章浩
    • Organizer
      園芸学会
    • Place of Presentation
      福井県立大学
    • Year and Date
      2012-09-22
    • Related Report
      2012 Annual Research Report
  • [Presentation] 寺上伸吾ナシの連鎖地図XVIII.SNPアレイを用いたニホンナシ高密度連鎖2012

    • Author(s)
      寺上伸吾
    • Organizer
      園芸学会
    • Place of Presentation
      福井県立大学
    • Year and Date
      2012-09-22
    • Related Report
      2012 Annual Research Report
  • [Presentation] RNAseq情報から作成した55,000グローブを用いた枝変わり大果変異セイヨウナシのトランスクリプトーム解析 第一報 : 開花前の花床におけるトランスクリプトーム解析2012

    • Author(s)
      加藤茉莉
    • Organizer
      園芸学会
    • Place of Presentation
      福井県立大学
    • Year and Date
      2012-09-22
    • Related Report
      2012 Annual Research Report
  • [Presentation] セイヨウナシ'ラ・フランス'果実における収穫後のデンプンの消失に関する研究2012

    • Author(s)
      村山秀樹
    • Organizer
      園芸学会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2012-03-23
    • Related Report
      2012 Annual Research Report
  • [Presentation] 落葉果樹のDNAマーカー、分子育種、ゲノム育種に関する研究

    • Author(s)
      山本俊哉
    • Organizer
      第52回ガンマーフィールドシンポジウム
    • Place of Presentation
      農業生物資源研究所
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Book] Abiotic Stress Biology in Horticultural Plants.2015

    • Author(s)
      Itai A.
    • Total Pages
      220
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Book] 果樹園芸学2015

    • Author(s)
      板井章浩
    • Total Pages
      224
    • Publisher
      朝倉書店
    • Related Report
      2014 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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