Functional analysis and its application of transcription factors responsible for fruit ripening using array technique, transgenic and TILLING technologies

• Project/Area Number: 24380023
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Horticulture/Landscape architecture
• Research Institution: Okayama University
• Principal Investigator: KUBO Yasutaka 岡山大学, その他の研究科, 教授 (80167387)
• Co-Investigator(Kenkyū-buntansha): YANO Kentaro 明治大学, 農学部, 准教授 (00446543)
• Co-Investigator(Renkei-kenkyūsha): NAKANO Ryohei 岡山大学, 大学院環境生命科学研究科, 准教授 (70294444)
• Research Collaborator: Yan Rui ; Mworia Eric G. ; Asiche William O.
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2012: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
• Keywords: 果実発育・成熟 / エチレン / アレイ解析 / 形質転換体 / 果実成熟 / 形質転換 / 転写因子 / TILLING

Outline of Final Research Achievements

Understanding of regulatory mechanism in fruit ripening is central hot point in horticultural physiology and critical point for technology development of storage and transportation. Using a tomato DNA macroarray consisting of 11,520 genes, we identified 419 ripening-associated genes (224 upregulated ,195 downregulated) and isolated 8 key transcription factors. GRAS-suppressed RNAi transgenic tomato exhibited down-regulation of PG and PL genes, suggesting That GRAS plays a key role for regulation of fruit ripening, in particular for softening process.

Research Products

• [Journal Article] Extension of Shelf-life by Limited Duration of Propylene and 1-MCP Treatments in Three Kiwifruit Cultivars (2016)
  • Author(s): Asiche, W., Mworia, E. G., Oda, C., Mitalo, O. W., Owino, W. O., Ushijima, K., Nakano, R., Kubo, Y.
  • Journal Title: The Horticulture Journal Volume: 85 Issue: 1 Pages: 76-85
  • DOI: 10.2503/hortj.MI-066
  • NAID: 130005121331
  • ISSN: 2189-0102, 2189-0110
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Plant omics data center: an integrated web repository for interspecies gene expression networks with NLP-based curation. (2015)
  • Author(s): Ohyanagi, H., Takano, T., Terashima, S., Kobayashi, M., Kanno, M., Morimoto, K., Matsumura, H., Sasaki, Y., Aya, K., Suwabe, K., Suzuki, G., Watanabe, M., Matsuoka, M., Yokoyama, K., Yano, K.
  • Journal Title: Plant and Cell Physiology Volume: 56 Issue: 1 Pages: 1-8
  • DOI: 10.1093/pcp/pcu188
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Characterization of ripening-asoociated genes using a tomato DNA macroarray, 1-methycyclopropene, and ripening-impaired mutants (2013)
  • Author(s): Yan R, Yokotani N, Yamaoka T, Ushijima K, Nakano R, Yano K,Aoki K, Kubo Y
  • Journal Title: Postharvest Biology and Technology Volume: 86 Pages: 159-170
  • DOI: 10.1016/j.postharvbio.2013.06.028
  • Peer Reviewed
• [Presentation] Characterization of ripening-associated genes using a tomato DNA macroarray, 1-methylcyclopropene in wild type, rin and nor tomato fruit (2015)
  • Author(s): Kubo, Y. ,Yan, R., Yokotani, N., Yamaoka, T., Ushijima K., Nakano, R. Yano, K. Aoki, K.
  • Organizer: The 10th International Conference on the Plant Hormone ethylene 2015
  • Place of Presentation: Chongqing, China
  • Year and Date: 2015-11-14
  • Int'l Joint Research
• [Presentation] Innovation in agricultural sector –past, present and future (2014)
  • Author(s): Kubo Y.
  • Organizer: The ninth JKUAT scientific, technological and industrialization conference
  • Place of Presentation: Jomokenyatta Univ. in Kenya
  • Year and Date: 2014-11-13
  • Invited
• [Presentation] Distinction between ethylene-dependent and low temperature modulated ripening in ‘Sanuki Gold’ Kiwifruit (2014)
  • Author(s): Asiche A.O., Kasahara Y. Kusakabe Y. Ushijima K. Nakano R. and Kubo
  • Organizer: 11th JSOL International Symposium on Solanaceae Genomics
  • Place of Presentation: 名古屋大学
  • Year and Date: 2014-10-25 – 2014-10-26
• [Presentation] DNAアレイによるトマト果実成熟機構の網羅的解析 (2014)
  • Author(s): 久保康隆・閻 瑞・村田綾香・牛島幸一郎・中野龍平
  • Organizer: 平成２６年度園芸学会中四国支部会
  • Place of Presentation: 徳島県農林水産総合技術支援センター
  • Year and Date: 2014-07-21
• [Presentation] Propylene and 1-MCP treatments in determining optimum ripening of three kiwifruitcultivars (2013)
  • Author(s): Asiche O.W., C. Oda, M. Tanigawa, K. Ushijima, R. Nakano. and Y. Kubo
  • Organizer: 園芸学会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2013-03-23
• [Presentation] トマト果実におけるAP3サブグループ遺伝子の機能解析 (2013)
  • Author(s): 山岡、竹重、荒木、久保、牛島、中野
  • Organizer: 園芸学会平成２５年度秋季大会
  • Place of Presentation: 岩手大学
• [Presentation] Functional analysis of tomato AP3-subbroup gene in fruit (2013)
  • Author(s): Yamaoka T, Araki K, Ushijima K, Kubo Y, Nakano R
  • Organizer: Japan Solanaceae Consortium (JSOL) workshop
  • Place of Presentation: Osaka Prefecture University
• [Presentation] Postharvest control of stress-induced fruit ripening in horticultural crops (2012)
  • Author(s): Kubo y E. G Mworia, W. O. Asiche, K. Ushijima, R. Nakano
  • Organizer: 7_<th> JKUAT Conference
  • Place of Presentation: JKUAT (Kenya)(招待講演)
  • Year and Date: 2012-11-16
• [Presentation] Low temperature modulated fruit ripening regulated by ethylene independent system in three kiwifruit cultivars (2012)
  • Author(s): Asiche O.W., C. Oda, M. Tanigawa, K. Ushijima, R. Nakano and Y Kubo
  • Organizer: 園芸学会
  • Place of Presentation: 福井県立大学
  • Year and Date: 2012-09-22
• [Book] Abiotic stress biology in horticultural crops. Ethylene, oxygen, carbon dioxide, and temperature in postharvest physiology (2015)
  • Author(s): Kubo, Y
  • Total Pages: 17
  • Publisher: Springer
• [Book] Ethylene, oxygen, carbon dioxide, and temperature in postharvest physiology. In Kanayama Y. and Kochetov A. eds. Abiotic stress biology in horticultural plants (2015)
  • Author(s): Kubo Y.
  • Publisher: Springer