Functional analysis and its application of transcription factors responsible for fruit ripening using array technique, transgenic and TILLING technologies
Project/Area Number |
24380023
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Horticulture/Landscape architecture
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Research Institution | Okayama University |
Principal Investigator |
KUBO Yasutaka 岡山大学, その他の研究科, 教授 (80167387)
|
Co-Investigator(Kenkyū-buntansha) |
YANO Kentaro 明治大学, 農学部, 准教授 (00446543)
|
Co-Investigator(Renkei-kenkyūsha) |
NAKANO Ryohei 岡山大学, 大学院環境生命科学研究科, 准教授 (70294444)
|
Research Collaborator |
Yan Rui
Mworia Eric G.
Asiche William O.
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Project Period (FY) |
2012-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2012: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
|
Keywords | 果実発育・成熟 / エチレン / アレイ解析 / 形質転換体 / 果実成熟 / 形質転換 / 転写因子 / TILLING |
Outline of Final Research Achievements |
Understanding of regulatory mechanism in fruit ripening is central hot point in horticultural physiology and critical point for technology development of storage and transportation. Using a tomato DNA macroarray consisting of 11,520 genes, we identified 419 ripening-associated genes (224 upregulated ,195 downregulated) and isolated 8 key transcription factors. GRAS-suppressed RNAi transgenic tomato exhibited down-regulation of PG and PL genes, suggesting That GRAS plays a key role for regulation of fruit ripening, in particular for softening process.
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Report
(5 results)
Research Products
(14 results)
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[Journal Article] Extension of Shelf-life by Limited Duration of Propylene and 1-MCP Treatments in Three Kiwifruit Cultivars2016
Author(s)
Asiche, W., Mworia, E. G., Oda, C., Mitalo, O. W., Owino, W. O., Ushijima, K., Nakano, R., Kubo, Y.
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Journal Title
The Horticulture Journal
Volume: 85
Issue: 1
Pages: 76-85
DOI
NAID
ISSN
2189-0102, 2189-0110
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Plant omics data center: an integrated web repository for interspecies gene expression networks with NLP-based curation.2015
Author(s)
Ohyanagi, H., Takano, T., Terashima, S., Kobayashi, M., Kanno, M., Morimoto, K., Matsumura, H., Sasaki, Y., Aya, K., Suwabe, K., Suzuki, G., Watanabe, M., Matsuoka, M., Yokoyama, K., Yano, K.
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Journal Title
Plant and Cell Physiology
Volume: 56
Issue: 1
Pages: 1-8
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Characterization of ripening-associated genes using a tomato DNA macroarray, 1-methylcyclopropene in wild type, rin and nor tomato fruit2015
Author(s)
Kubo, Y. ,Yan, R., Yokotani, N., Yamaoka, T., Ushijima K., Nakano, R. Yano, K. Aoki, K.
Organizer
The 10th International Conference on the Plant Hormone ethylene 2015
Place of Presentation
Chongqing, China
Year and Date
2015-11-14
Related Report
Int'l Joint Research
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