Elucidation of the molecular mechanisms regulating flowerl pigmentation in gentian plants

• Project/Area Number: 24380024
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Horticulture/Landscape architecture
• Research Institution: Iwate Biotechnology Research Center
• Principal Investigator: NISHIHARA MASAHIRO 公益財団法人岩手生物工学研究センター, 園芸資源研究部, 研究部長 (20390883)
• Co-Investigator(Kenkyū-buntansha): SASAKI Nobuhiro (公財)岩手生物工学研究センター, 園芸資源研究部, 主任研究員 (80422088) ; TAKAHASHI Hideyuki (公財)岩手生物工学研究センター, 園芸資源研究部, 主任研究員 (00455247) ; NAKATSUKA Takashi 静岡大学, 農学部, 助教 (60435576)
• Research Collaborator: YAMADA Eri ; FUJITA Kohei
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2012: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
• Keywords: リンドウ / 花色 / 転写因子 / フラボノイド / フラボン / トランスポゾン / アントシアニン / MYB / 植物 / 遺伝子 / ゲノム

Outline of Final Research Achievements

We attempted to elucidate the molecular mechanisms that control flower pigmentation in Japanese cultivated gentians (Gentiana triflora, G. scabra and their hybrids). Four novel MYB transcription factor genes (GtMYBP3,GtMYBP4, GtMYBR1R1 and GtMYB1R9) were newly identified and their functions on the transcriptional regulation of flavonoid biosynthesis in flowers were investigated. Furthermore, we determined the structures of flavone pigments accumulated in gentian leaves and flower petals and also first identified a novel glucosyltransferase gene (GtUF6CGT1) involved in C-glucosylflavone formation. We also determined several transposon-like sequences inserted into the flavonoid biosynthesis-related genes that might cause flower color mutation such as pink and white. These data provide basic information to understand flower pigmentation in gentian plants.

Research Products

• [Journal Article] Identification of the glucosyltransferase that mediates direct flavone C-glucosylation in Gentiana triflora (2015)
  • Author(s): Sasaki, N., Nishizaki, Y., Yamada, E., Tatsuzawa, F., Nakatsuka, T. Takahashi, H., Nishihara, M.
  • Journal Title: FEBS Letters Volume: 589 Issue: 1 Pages: 182-187
  • DOI: 10.1016/j.febslet.2014.11.045
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Transcriptional regulators of flavonoid biosynthesis and their application to flower color modification in Japanese gentians (2014)
  • Author(s): Nakatsuka, T., Sasaki, N., Nishihara, M.
  • Journal Title: Plant Biotechnology Volume: 31 Issue: 5 Pages: 389-399
  • DOI: 10.5511/plantbiotechnology.14.0731a
  • NAID: 130004721157
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 植物の香りと色の代謝工学が拓く新時代 (2014)
  • Author(s): 有村源一郎・西原昌宏・下田武志
  • Journal Title: バイオサイエンスとインダストリー Volume: 72 Pages: 197-202
  • NAID: 40020102376
• [Journal Article] Suppression of anthocyanin pigmentation by single MYB transcription factors from gentian. (2013)
  • Author(s): Takashi Nakatsuka, Eri Yamada, Misa Saito, Kohei Fujita and Masahiro Nishihara
  • Journal Title: Plant Cell Reports Volume: 32 Issue: 12 Pages: 1925-1937
  • DOI: 10.1007/s00299-013-1504-4
  • Peer Reviewed
• [Journal Article] Isolation and characterisation of GtMYBP3 and GtMYBP4, orthologues of R2R3-MYB transcription factors that regulate early flavonoid biosynthesis, in gentian flowers. (2012)
  • Author(s): Nakatsuka, T., Saito, M., Yamada, E., Fujita, K., Kakizaki, Y. and Nishihara, M
  • Journal Title: Journal of Experimental Botany Volume: 63 Issue: 18 Pages: 6505-6517
  • DOI: 10.1093/jxb/ers306
  • Peer Reviewed
• [Presentation] エゾリンドウからのフラボン配糖化酵素遺伝子群の単離 (2015)
  • Author(s): 佐々木 伸大,山田 恵理,西﨑 雄三,中塚 貴司,立澤 文見,樋口 敦美,藤田 晃平,高橋 秀行, 西原 昌宏
  • Organizer: 日本植物生理学会第56回年会
  • Place of Presentation: 東京農業大学世田谷キャンパス
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 日本のリンドウ園芸品種に含まれるポリフェノール類の解析 (2014)
  • Author(s): 佐々木伸大、山田恵理、西﨑雄三、中塚貴司、立澤文見、樋口敦美、藤田晃平、高橋秀行、西原昌宏
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 明治大学生田キャンパス
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] リンドウからのフラボンC－配糖化酵素の単離 (2014)
  • Author(s): 佐々木伸大、山田恵理、西﨑雄三、中塚貴司、立澤文見、樋口敦美、藤田晃平、 高橋秀行、西原昌宏
  • Organizer: 日本植物細胞分子生物学会第32回大会・シンポジウム
  • Place of Presentation: いわて県民情報交流センター
  • Year and Date: 2014-08-21 – 2014-08-22
• [Presentation] リンドウ花弁のフラボノイド生合成の初期経路を制御するP1型MYB転写因子の解析 (2013)
  • Author(s): 中塚貴司、斎藤美沙、山田恵理、藤田晃平、柿崎裕子、西原昌宏
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学津島キャンパス
  • Year and Date: 2013-03-21
• [Presentation] リンドウcDNAライブラリーの構築 (2013)
  • Author(s): 中塚貴司、山田恵理、今村智弘、高橋秀行、斎藤美沙、藤田晃平、西原昌宏
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学津島キャンパス
  • Year and Date: 2013-03-21
• [Presentation] リンドウ花弁で発現するSingle-repeat MYB転写因子遺伝子の機能解析 (2013)
  • Author(s): 中塚貴司、山田恵理、齋藤美沙、藤田晃平、佐々木伸大、西原昌宏
  • Organizer: 日本植物細胞分子生物学会第31回大会シンポジウム
  • Place of Presentation: 北海道大学札幌キャンパス
• [Presentation] リンドウの花色発色に関わる転写調節因子の解析
  • Author(s): 西原昌宏
  • Organizer: 第2回植物二次代謝フロンティア研究会
  • Place of Presentation: 奈良県新公会堂
  • Invited
• [Presentation] DNAマーカーを利用したリンドウの品種識別と育種の効率化
  • Author(s): 西原昌宏
  • Organizer: 園芸学会平成２５年度秋季大会 小集会 園芸作物におけるDNAマーカー開発と利用
  • Place of Presentation: 岩手大学
  • Invited
• [Book] The Gentianaceae : Volume 2 - Biotechnology and Applications Chapter10 Molecular breeding of Japanese gentians - Applications of genetic transformation, metabolome analyses, and genetic markers. (2015)
  • Author(s): Nishihara, M., Mishiba, K., Imamura, T., Takahashi,, H. and Nakatsuka, T.
  • Total Pages: 452
  • Publisher: Springer