| Project/Area Number |
24380026
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant pathology
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| Research Institution | Hirosaki University |
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Principal Investigator |
SANO Teruo 弘前大学, 農学生命科学部, 教授 (30142699)
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| Co-Investigator(Kenkyū-buntansha) |
HARADA Takeo 弘前大学, 農学生命科学部, 教授 (10228645)
TANEDA Akito 弘前大学, 理工学研究科, 准教授 (70332492)
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| Research Collaborator |
OWENS R.A. USDA, Agriculture Research Service Molecular Plant Pathology Laboratory
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Keywords | ウイロイド / ノンコーディングRNA / RNAサイレンシング / siRNAシークエンス / 抵抗性 / 病原性 / 非コードRNA / スモールRNA / ウイロイド抵抗性 / ウイロイド特異的スモールRNA / マイクロRNA / ヘアピンRNA |
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| Outline of Final Research Achievements |
Deep sequencing analysis of PSTVd-small RNA in leaf and stem of tomato infected with severe and mild PSTVd isolates revealed that PSTVd-small RNA was less abundant in leaf tissues than in stem, and in those infected with mild isolate than with severe. Reduction of PSTVd-small RNA in mild isolate was conspicuous especially in the regions containing the nucleotides 118, 126 and 201. Relative expression levels of some host microRNAs were down-regulated in PSTVd infected plants. Especially, miR159 decreased to nearly 50% and miR319 decreased to 33-63% of that of the healthy.
Transgenic N.benthamiana and tomato lines expressing varieties of hairpin RNAs were created, which included various PSTVd-derived sequences, tomato gibberellin-related gene, DCL2 gene and AGO2 gene. Four of the 21 lines of transgenic N.benthamiana expressing hairpin RNA of PSTVd-related sequences showed delay of the infection and reduction of PSTVd accumulation.
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