Elucidation of global network of regulatory mechanism in virulence-related gene expression of phytopathogenic bacteria

• Project/Area Number: 24380028
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Okayama University
• Principal Investigator: ICHINOSE YUKI 岡山大学, その他の研究科, 教授 (50213004)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2013: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
• Keywords: 病原力遺伝子 / 転写因子 / 菌体密度感知機構 / HrpタイプIII分泌システム / MarR / 遺伝子発現制御機構 / PsyR / Vfr / ArsR / 病原性遺伝子 / ネットワーク / 発現制御機構 / Hrpタイプ3分泌システム

Outline of Final Research Achievements

Motility in Pseudomonas syringae requires flagella and type IV pili (T4P). Flagella and T4P are required for production of acyl homoserine lactones (AHL), quorum sensing molecules and cAMP, respectively. Production of AHL and cAMP also requires transcription factors AefR and PsyR and Vfr, respectively. Defect of aefR or psyR resulted in the enhancement of multidrug efflux pump genes mexEF/oprN, whereas defect of vfr resulted in the enhancement of another pump genes mexAB/oprM. Enhancement of both pump genes activated multidrug tolerance. PsyR and Vfr are also involved in the regulation of hrp gene expression.

Research Products

• [Journal Article] Characterization of quorum sensing-controlled transcriptional regulator MarR and Rieske (2Fe-2S) cluster-containing protein (Orf5) that are involved in resistance to environmental stresses in Pseudomonas syringae pv. tabaci 6605 (2015)
  • Author(s): Taguchi, F., Inoue, Y., Suzuki, T., Inagaki, Y., Yamamoto, M., Toyoda, K., Yoshiteru, N., Shiraishi, T. and Ichinose, Y.
  • Journal Title: Molecular Plant Pathology Volume: 16 Issue: 4 Pages: 376-387
  • DOI: 10.1111/mpp.12187
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Pathogenicity and virulence factors of <i>Pseudomonas syringae</i>. (2014)
  • Author(s): 一瀬勇規・田口富美子・向原隆文
  • Journal Title: Japanese Journal of Phytopathology Volume: 80 Issue: 100th_Anniversary Pages: S97-S103
  • DOI: 10.3186/jjphytopath.80.S97
  • NAID: 130004779394
  • ISSN: 0031-9473, 1882-0484
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Pathogenicity and virulence factors of Pseudomonas syringae. (2013)
  • Author(s): Ichinose Y, Taguchi F, Mukaihara T
  • Journal Title: Journal of General Plant Pathology Volume: 79 Issue: 5 Pages: 285-296
  • DOI: 10.1007/s10327-013-0452-8
  • NAID: 10031195193
  • Peer Reviewed
• [Journal Article] Virulence factor regulator (Vfr) controls virulence-associated phenotypes in Pseudomonas syringae pv. tabaci 6605 by a quorum sensing-independent mechanism. (2013)
  • Author(s): Taguchi, F. and Ichinose, Y.
  • Journal Title: Molecular Plant Pathology Volume: 14 Issue: 3 Pages: 279-292
  • DOI: 10.1111/mpp.12003
  • Peer Reviewed
• [Journal Article] Characterization of each aefR and mexT mutant in Pseudomonas syringae pv. tabaci 6605. (2012)
  • Author(s): Kawakita, et al.
  • Journal Title: Molecular Genetics and Genomics Volume: 287 Issue: 6 Pages: 473-484
  • DOI: 10.1007/s00438-012-0693-9
  • Peer Reviewed
• [Presentation] Pseudomonas syringae pv. tabaci 6605の菌体密度感知機構転写制御因子PsyRによるpsyI並びにhrpLの転写制御 (2015)
  • Author(s): 高田基弘・田口富美子・山本幹博・能年義輝・豊田和弘・稲垣善茂・一瀬勇規
  • Organizer: 平成27年度日本植物病理学会大会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-29 – 2015-03-31
• [Presentation] Pseudomonas syringae pv. tomato DC3000の酸化ストレス応答性転写因子OxyRは本菌の病原力に貢献する (2015)
  • Author(s): 石賀康博・一瀬勇規・澤田貴博
  • Organizer: 平成27年度日本植物病理学会大会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-29 – 2015-03-31
• [Presentation] Characterization of transcriptional regulator MarR and Rieske (2Fe-2S) domain-containing protein of Pseudomonas syringae pv. tabaci involved in virulence and resistance to environmental stresses (2014)
  • Author(s): Yuki Ichinose and Fumiko Taguchi
  • Organizer: 3rd Korea-Japan joint Symposium
  • Place of Presentation: Pusan, Korea
  • Year and Date: 2014-10-23 – 2014-10-24
• [Presentation] Pseudomonas syringae pv. tabaciにおける病原力制御因子ArsRの機能解析 (2014)
  • Author(s): 澤田貴博・田口富美子・山本幹博・稲垣善茂・能年義輝・豊田和弘・一瀬勇規
  • Organizer: 平成26年度日本植物病理学会関西部会
  • Place of Presentation: 富山
  • Year and Date: 2014-09-27 – 2014-09-28
• [Presentation] Pseudomonas syringaeの病原性関連遺伝子の発現制御ネットワーク (2014)
  • Author(s): 一瀬勇規・田口富美子
  • Organizer: 平成26年度植物感染生理談話会
  • Place of Presentation: 仙台
  • Year and Date: 2014-08-06 – 2014-08-08
  • Invited
• [Presentation] Characteriazation of Transcriptional regulator MarR and Rieske (2Fe-2S) Domain-Containing Protein od Pseudomonas syringae pv. tabaci involved in Virulence and Resistance to Environmental Stresses (2014)
  • Author(s): Yuki ICHINOSE, Fumiko TAGUCHI
  • Organizer: IS-MPMI 2014 XVI International Congress
  • Place of Presentation: Rhodes, Greece
  • Year and Date: 2014-07-06 – 2014-07-10
• [Presentation] Regulation of motility-mediated virulence in Pseudomonas syringae pv. tabaci 6605 (2014)
  • Author(s): Yuki ICHINOSE, Fumiko TAGUCHI
  • Organizer: The 13th International Conference of Plant Pathogenic Bacteria (ICPPB)
  • Place of Presentation: 上海，中国
  • Year and Date: 2014-06-08 – 2014-06-13
• [Presentation] Pseudomonas syringae pv. syringae B728aの病害力制御因子Vfrの標的遺伝子のクロマチン免疫沈降法による探索 (2014)
  • Author(s): 小倉敬右・田口富美子・山本幹博・能年義輝・豊田和弘・稲垣善茂・一瀬勇規
  • Organizer: 平成26年度日本植物病理学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2014-06-02 – 2014-06-04
• [Presentation] Pseudomonas syringae pv. tabaciにおけるストレス応答に関与する転写制御因子MarRの機能解析 (2013)
  • Author(s): 田口富美子・井上優子・山本幹博・稲垣善茂・豊田和弘・白石友紀・一瀬勇規
  • Organizer: 平成25年度日本植物病理学会関西部会
  • Place of Presentation: 岡山
• [Presentation] Pseudomonas syringaeの病原性と環境応答 (2013)
  • Author(s): 一瀬勇規・田口富美子
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉(招待講演)
• [Presentation] Pseudomonas syringaeのVfrによる病原性制御は、菌体密度感知機構に依存しない (2013)
  • Author(s): 田口富美子・一瀬勇規
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉
• [Presentation] Pseudomonas syringae pv. tabaciの多剤耐性制御因子MarRの機能解析 (2013)
  • Author(s): 井上優子・田口富美子・一瀬勇規
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉