| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2013: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Outline of Final Research Achievements |
Motility in Pseudomonas syringae requires flagella and type IV pili (T4P). Flagella and T4P are required for production of acyl homoserine lactones (AHL), quorum sensing molecules and cAMP, respectively. Production of AHL and cAMP also requires transcription factors AefR and PsyR and Vfr, respectively. Defect of aefR or psyR resulted in the enhancement of multidrug efflux pump genes mexEF/oprN, whereas defect of vfr resulted in the enhancement of another pump genes mexAB/oprM. Enhancement of both pump genes activated multidrug tolerance. PsyR and Vfr are also involved in the regulation of hrp gene expression.
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