| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2014: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2013: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2012: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
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| Outline of Final Research Achievements |
The 130K replication protein of a tobamovirus bound to a 5'-terminal ca. 70-nucleotide region of its genomic RNA to form a ribonucleoprotein complex named PMTC. The binding inhibited translation of the RNA and lead it to a replication pathway. The PMTC contained multiple molecules of the replication protein. Interestingly, free (non-membrane-bound) 130K protein did not bind tobamovirus RNA, but the Met-IR fragment that corresponded to the N-terminal two-thirds of the 130K protein did. Thus, 130K protein molecules likely recognize tobamovirus RNA before the completion of their synthesis.
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