Molecular mechanism of induction of embryonic diapause in Bombyx mori
| Project/Area Number |
24380031
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Shinshu University |
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Principal Investigator |
SHIOMI Kunihiro 信州大学, 学術研究院繊維学系, 准教授 (70324241)
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| Co-Investigator(Kenkyū-buntansha) |
MIZOGUCHI Akira 愛知学院大学, 教養部, 教授 (60183109)
OHTA Hiroto 熊本大学, 自然科学研究科, 助教 (60450334)
SHIRAI Koji 信州大学, 学術研究院繊維学系, 准教授 (00293499)
NAKANISHI Hiromitsu 信州大学, サテライト・ベンチャー・ビジネス・ラボラトリー, 助教 (90443001)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Keywords | カイコ / 温度センサー / RNA-seq. 解析 / 休眠ホルモン / カイコ (Bombyx mori) / 休眠 / 温度感受性 TRP チャネル / ゲノム編集 |
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| Outline of Final Research Achievements |
In the bivoltine strain of the silkworm, Bombyx mori, embryonic diapause is induced transgenerationally as a maternal effect. Progeny diapause is determined by the environmental temperature during embryonic development of the mother; however, its molecular mechanisms are largely unknown. Here, we show that the Bombyx TRPA1 ortholog (BmTrpA1) acts as a thermosensitive transient receptor potential (TRP) channel that is activated at temperatures above ~21°C and affects the induction of diapause in progeny. In addition, we show that embryonic RNAi of BmTrpA1 affects diapause hormone release during pupal-adult development. Furthermore, we searched the candidate genes participating in diapause induction. This is the first study to identify a thermosensitive TRP channel that acts as a molecular switch for a relatively long-term predictive adaptive response by inducing an alternative phenotype to seasonal polyphenism.
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Report
(5 results)
Research Products
(19 results)
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[Journal Article] Disruption of diapause induction by TALEN-based gene mutagenesis in relation to a unique neuropeptide signaling pathway in Bombyx.2015
Author(s)
Shiomi, K., Takasu, Y., Kunii, M., Tsuchiya, R., Mukaida, M., Kobayashi, M., Sezutsu, H., Ichida, M., Mizoguchi, A.
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Journal Title
Scientific Reports
Volume: 5
Issue: 1
Pages: 15566-15566
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Embryonic thermosensitive TRPA1 determines transgenerational diapause phenotype of the silkworm, Bombyx mori.2014
Author(s)
Sato, A., Sokabe, T., Kashio, M., Yasukochi, Y., Tominaga, M., Shiomi, K.
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Journal Title
Proc Natl Acad Sci U S A
Volume: 111(13)
Issue: 13
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A baculovirus immediate-early gene, ie1, promoter drives efficient expression of a transgene in both Drosophila melanogaster and Bombyx mori2012
Author(s)
Masumoto, M., Ohde, T., Shiomi, K., Yaginuma, T. and Niimi, T.
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Journal Title
PLoS ONE
Volume: 7
Issue: 11
Pages: e49323-e49323
DOI
NAID
Related Report
Peer Reviewed
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