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Analysis of encapsulation in cellular defence mechanism of lepidopteran larva

Research Project

Project/Area Number 24380032
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Applied entomology
Research InstitutionNagoya University

Principal Investigator

TANAKA Toshiharu  名古屋大学, 生命農学研究科, 教授 (30227152)

Co-Investigator(Kenkyū-buntansha) KAWAKITA Kazuhito  名古屋大学, 大学院生命農学研究科, 教授 (90186065)
KITAJIMA Ken  名古屋大学, 生物機能開発利用センター, 教授 (80192558)
MINAKUCHI Chieka  名古屋大学, 大学院生命農学研究科, 助教 (90509134)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Keywords細胞性免疫 / 包囲作用 / メラニン化 / 免疫レクチン / Cotesia kariyai / Meteorus pluchricornis / 内部寄生蜂 / polydnavirus / venom / virus like particle / immune lectin / Cotesia ruficrus / Meteorus plucricornis / virus-like particle / 細胞接着阻害 / 細胞性防御反応 / 液性防御反応 / focal adhesion complex
Outline of Final Research Achievements

Insects have cellular defence system against parasites or foreign substances invaded into the body hemocoel. However, some endoparasitoids can avoid the encapsulation of the host by polydnavirus (PDV) plus venom injected into the host hemocoel at oviposition and teratocytes (Tc) that develop from serosal cells of the egg during larval stage. It was found that hyper spread cells of the Mythimna separata host (Ms-HSC) attached on the surface of the foreign substance like glass slide or of parasitoid larva, and made melanin formation that works as marker to another hemocytes. On the other hand, both PDV and venom also teratocytes produced factor inhibiting the function of HSC, especially venom worked in very early stage of parasitization.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (19 results)

All 2015 2014 2013 2012

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (12 results)

  • [Journal Article] Role of scalloped in the post-embryonic development of the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae)2015

    • Author(s)
      Minakuchi, C. T. Ohde, K. Miura, T. Tanaka, T. Niimi.
    • Journal Title

      Appl. Entomol. Zool.

      Volume: 50 Issue: 1 Pages: 17-26

    • DOI

      10.1007/s13355-014-0297-4

    • NAID

      40020374251

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Kato, Y. T. Yoshida, K. Miura, T. Tanaka, Y. Nakamatsu, M. Ochiai. A novel type of hemocytes localizing melanization with high-spreading behavior in Mythimna separata.2014

    • Author(s)
      Kato, Y. T. Yoshida, K. Miura, T. Tanaka, Y. Nakamatsu, M. Ochiai
    • Journal Title

      Archiv. Insect Biochem. Physiol.

      Volume: 86 Issue: 4 Pages: 220-239

    • DOI

      10.1002/arch.21173

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Duplication of acetylcholinesterase gene in diamondback moth strains with different sensitivities to acephate2014

    • Author(s)
      Shoji Sonodaa, Xueyan Shib, Dunlun Songb, Pei Liangb, Xiwu Gaob, Youjun Zhangc, Jianhong Lid, Yong Liue, Ming Lif, Masaya Matsumurag, Sachiyo Sanadag, Chieka Minakuchih, Toshiharu Tanakah, Tadashi Miyata
    • Journal Title

      Insect Biochemistry and Molecular Biology

      Volume: 48 Pages: 83-90

    • DOI

      10.1016/j.ibmb.2014.02.008

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inter- and intraspecific host discrimination in gregarious and solitary endoparasitoid wasps.2013

    • Author(s)
      Peter M. Magdaraog, T. Tanaka, J. A.
    • Journal Title

      BioControl

      Volume: 58 Issue: 6 Pages: 745-754

    • DOI

      10.1007/s10526-013-9532-0

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of NF-kappaB transcription factors in antimicrobial peptide gene induction in the red flour beetle, Tribolium castaneum.2012

    • Author(s)
      Kakeru Yokoi, Hiroaki Koyama, Wataru Ito, Chieka minakuchi, Toshiharu Tanaka, Ken Miura
    • Journal Title

      Developmental and Comparative Immunology

      Volume: 38 Issue: 2 Pages: 342-351

    • DOI

      10.1016/j.dci.2012.06.008

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antimicrobial peptide gene induction, involvement of Toll and IMD pathways and defense against bacteria in the red flour beetle, Tribolium castaneum.2012

    • Author(s)
      Kakeru Yokoi, Hiroaki Koyama, Chieka minakuchi, Toshiharu Tanaka, Ken Miura
    • Journal Title

      Results in Immunology

      Volume: 2 Pages: 72-83

    • DOI

      10.1016/j.rinim.2012.03.002

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intrinsic inter-and intraspecific competition in parasitoid wasps2012

    • Author(s)
      JA. Harvey, EH. Poleman, T. Tanaka
    • Journal Title

      Annu. Rev. Entomol.

      Volume: 58 Issue: 1 Pages: 333-351

    • DOI

      10.1146/annurev-ento-120811-153622

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Role of the juvenile hormone in the establishment of sexal dimorphism in the Japanese mealybug2015

    • Author(s)
      Isabelle Vea, Shiotuki Takahiro, Joraku Akiya , Tanaka, Toshiharu, Minakuchi Chieka
    • Organizer
      日本応用動物昆虫学会
    • Place of Presentation
      山形大学
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Expression analysis of the doublesex gene in the Japanese mealybug2015

    • Author(s)
      Minakuchi Chieka, Shiotuki Takahiro, Joraku Akira, Tanaka Toshiharu, Vea Isabelle、
    • Organizer
      日本応用動物昆虫学会
    • Place of Presentation
      山形大学
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Meteorus pulchricornis teratocyteの役割2015

    • Author(s)
      佐藤礼菜・加藤良晃・田中利治
    • Organizer
      日本応用動物昆虫学会
    • Place of Presentation
      山形大学
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] カリヤサムライコマユバチのポリドナウイルスが寄主の免疫反応に及ぼす影響2015

    • Author(s)
      澤友美・物部瞳・中松豊・加藤良晃・田中利治,
    • Organizer
      日本応用動物昆虫学会
    • Place of Presentation
      山形大学
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 貯穀害虫コクヌストモドキのIMD経路の分子解剖-続報2015

    • Author(s)
      横井翔・伊藤渉・加藤大貴・水口智江可・田中利治・三浦健,
    • Organizer
      日本応用動物昆虫学会
    • Place of Presentation
      山形大学
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] ミカンキイロアザミウマにおける転写因子e93のcDNA単離および発現解析2015

    • Author(s)
      鈴木洋平、塩月孝博、上樂明也・三浦健・田中利治・水口智江可,
    • Organizer
      日本応用動物昆虫学会
    • Place of Presentation
      山形大学
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] コクヌストモドキの昆虫病原性糸状菌に対する免疫応答-I2015

    • Author(s)
      加藤大貴・早川優輝・水口智江可・田中利治・横井翔・神谷克巳・三浦健
    • Organizer
      日本応用動物昆虫学会
    • Place of Presentation
      山形大学
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] コクヌストモドキの昆虫病原性糸状菌に対する免疫応答-II2015

    • Author(s)
      早川優輝・加藤大貴・森彩乃・水口智江可・田中利治・横井翔・神谷克巳・三浦健,
    • Organizer
      日本応用動物昆虫学会
    • Place of Presentation
      山形大学
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] コクヌストモドキの免疫シグナル伝達経路の特性について2014

    • Author(s)
      三浦健、加藤大貴、小山裕明、水口智江可、田中利治、横井翔
    • Organizer
      第58回日本応用動物学会
    • Place of Presentation
      高知大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 異物のメラニン沈着を抑制するテラトサイトの働きについて2014

    • Author(s)
      澤友美、向冴子、森瑞紀、加藤良晃、立石剣、田中利治、中松豊
    • Organizer
      第58回日本応用動物学会
    • Place of Presentation
      高知大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] コクヌストモドキのToll遺伝子群の逆遺伝学的機能解析2014

    • Author(s)
      加藤大貴、小山裕明、水口智江可、田中利治、横井翔、三浦健
    • Organizer
      第58回日本応用動物学会
    • Place of Presentation
      高知大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 蜂の寄生が脂肪体の細胞遊出に及ぼす影響2013

    • Author(s)
      澤友美, 他8名
    • Organizer
      第57回応用動物昆虫学会
    • Place of Presentation
      日本大学生物資源科学部
    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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