| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
|
|---|
| Outline of Final Research Achievements |
In the silkworm (Bombyx mori), diapause hormone that is biosynthesized in the subesophageal ganglion is released into the hemolymph and acts on developing ovaries in the mid-pupal stage of females, in order to induce embryonic diapause that occurs 2-3 days after oviposition. As a diapause-initiating gene, we have already isolated the pnd (pigmented and nondiapausing egg) gene. Therefore, we here conducted the experiments to examine whether the insulin-signal transduction system is intervened between the diapause-signal and the expression of pnd gene. We show that the mRNA expression of insulin-like peptide (BmILP) is lowered in diapause-eggs than in nondiapause-eggs, and that the cis-element recognized and bound by FoxO transcriptional factor located at the downstream of insulin-signal transduction system is found in the 5’-upstream region of the pnd gene.
|
