Molecular mechanism intervened between the action of diapause hormone on developing ovaries and the expression of pnd gene that is the diapause-initiating gene
| Project/Area Number |
24380033
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NIIMI Teruyuki 基礎生物学研究所, 大学共同利用機関等の部局等, 教授 (00293712)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Keywords | 昆虫分子生物学 / 蚕糸学 / カイコ胚休眠 / 休眠ホルモン / 休眠開始遺伝子 / 遺伝子発現調節 / シグナル伝達系 / 昆虫分子生理学 / シグナル伝達 |
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| Outline of Final Research Achievements |
In the silkworm (Bombyx mori), diapause hormone that is biosynthesized in the subesophageal ganglion is released into the hemolymph and acts on developing ovaries in the mid-pupal stage of females, in order to induce embryonic diapause that occurs 2-3 days after oviposition. As a diapause-initiating gene, we have already isolated the pnd (pigmented and nondiapausing egg) gene. Therefore, we here conducted the experiments to examine whether the insulin-signal transduction system is intervened between the diapause-signal and the expression of pnd gene. We show that the mRNA expression of insulin-like peptide (BmILP) is lowered in diapause-eggs than in nondiapause-eggs, and that the cis-element recognized and bound by FoxO transcriptional factor located at the downstream of insulin-signal transduction system is found in the 5’-upstream region of the pnd gene.
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Report
(5 results)
Research Products
(11 results)
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[Journal Article] Cloning of cDNA encoding a Bombyx mori homolog of human oxidation resistance 1 (OXR1) protein from diapause eggs, and analyses of its expression and function2014
Author(s)
Kobayashi, N., Takahashi, M., Kihara, S., Niimi, T., Yamashita, O. and Yaginuma, T.
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Journal Title
Journl of Insect Physiology
Volume: 68
Pages: 58-68
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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