| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
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| Outline of Final Research Achievements |
To analyze biological functions of the gene that is thought to be responsible for manipulation of host development, we constructed recombinant viruses, namely knock-in virus created by inserting the green fluorescent protein reporter under the control of spheroidin gene promoter (GFP cassette) into a non-coding region of virus genome, knock-out virus having the gene disrupted by a GFP cassette and rescue virus that is restored the complete gene in knock-out virus. Phenotypic comparison of wild-type and recombinant viruses-infected larvae of Mythimna separata indicated that the gene is truly responsible for manipulation of host development and metamorphosis of the infected hosts. Furthermore, the gene altered normal behavior of the host before pupation. Non-infected larvae moved from host plants to soil and formed cell for pupation. However, insects infected with viruses having the gene did not show such behavior and stayed on host plants.
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