Analysis of defense mechanism against DNA viruses in the silkworm
| Project/Area Number |
24380036
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
TANAKA Hiromitsu 独立行政法人農業生物資源研究所, 昆虫微生物機能研究ユニット, ユニット長 (30391577)
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| Co-Investigator(Kenkyū-buntansha) |
TSUBOTA Takuya 独立行政法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, 任期付研究員 (00612772)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | カイコ / 核多角病ウイルス / テトラスパニン / インテグリン / Etsファミリータンパク質 / 昆虫免疫 / カイコ核多角体病ウイルス / カイコ多角体病ウイルス |
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| Outline of Final Research Achievements |
In this study, we analyzed the suppression mechanism of DNA viral propagation in response to DNA viral infection using a silkworm cell line and silkworm nucleopolyhedroviruses (BmNPV). We found that two predicted membrane proteins, tetraspanin and integrin, both of which genes are up-regulated in response to BmNPV infection, were involved in suppression of BmNPV propagation in the cells. We further demonstrated that tetraspanin regulated BmEts-dependent repression of expression of the viral immediate early gene. We believe that these findings contribute to elucidation of the mechanisms of insect immunity against DNA viruses.
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Report
(4 results)
Research Products
(2 results)
