Roles of autophagy in the life cycle of rice

• Project/Area Number: 24380037
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Tohoku University
• Principal Investigator: Ishida Hiroyuki 東北大学, (連合)農学研究科(研究院), 准教授 (60312625)
• Co-Investigator(Kenkyū-buntansha): MAKINO Amane 東北大学, 大学院農学研究科, 教授 (70181617)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2013: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2012: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
• Keywords: オートファジー / 窒素転流 / 栄養成長 / 生殖成長 / イネ / 葉緑体 / 老化 / 光合成 / 栄養飢餓

Outline of Final Research Achievements

We aimed to reveal roles of autophagy in the life cycle of rice and obtained following results. (1) We isolated autophagy-defective rice mutants. (2) We established monitoring methods for autophagy by generating transgenic rice expressing fluorescent protein markers. (3) We found that autophagy-defective rice mutants show delayed reproductive growth and heading and they show male sterile phenotype due to incomplete development of pollen and anther. (4) We found that autophagy-defective rice mutants show reduced biomass production and nitrogen use efficiency due to suppression of chloroplast protein degradation and nitrogen remobilisation.

Research Products

• [Journal Article] From Arabidopsis to cereal crops: Conservation of chloroplast protein degradation by autophagy indicates its fundamental role in plant productivity. (2015)
  • Author(s): Izumi M, Hidema J, Ishida H
  • Journal Title: Plant Signaling & Behavior Volume: 10 Issue: 11 Pages: e1101199-e1101199
  • DOI: 10.1080/15592324.2015.1101199
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Establishment of monitoring methods for autophagy in rice reveals autophagic recycling of chloroplasts and root plastids during energy limitation. (2015)
  • Author(s): Izumi M, Hidema J, Wada S, Kondo E, Kurusu T, Kuchitsu K, Makino A, Ishida H.
  • Journal Title: Plant Physiology Volume: 169 Issue: 4 Pages: 1307-1320
  • DOI: 10.1104/pp.114.254078
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Autophagy supports biomass production and nitrogen use efficiency at the vegetative stage in rice (2015)
  • Author(s): Wada S, Hayashida Y, Izumi M, Kurusu T, Hanamata S, Kanno K, Kojima S, Yamaya T, Kuchitsu K, Makino A, Ishida H
  • Journal Title: Plant Physiology Volume: in press Issue: 1 Pages: 60-73
  • DOI: 10.1104/pp.15.00242
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Roles of autophagy in chloroplast recycling (2014)
  • Author(s): Ishida H, Izumi M, Wada S and Makino A
  • Journal Title: Biochim. Biophys. Acta Volume: 1837 (4) Issue: 4 Pages: 512-521
  • DOI: 10.1016/j.bbabio.2013.11.009
  • Peer Reviewed
• [Journal Article] OsATG7 is required for autophagy-dependent lipid metabolism in rice postmeiotic anther development (2014)
  • Author(s): Takamitsu Kurusu, Tomoko Koyano, Shigeru Hanamata, Takahiko Kubo, Yuhei Noguchi, Chikako Yagi, Noriko Nagata, Takashi Yamamoto, Takayuki Ohnishi, Yozo Okazaki, Nobutaka Kitahata, Daichi Ando, Masaya Ishikawa, Shinya Wada, Akio Miyao, Hirohiko Hirochika, Hiroaki Shimada, Amane Makino, Kazuki Saito, Hiroyuki Ishida, Tetsu Kjnoshita, Nori Kurata and Kazuyuki Kuchitsu^*
  • Journal Title: Autophagy Volume: 10(5) Issue: 5 Pages: 878-888
  • DOI: 10.4161/auto.28279
  • Peer Reviewed / Open Access
• [Journal Article] Deficiency of autophagy leads to significant changes of metabolic profiles in Arabidopsis. (2013)
  • Author(s): Izumi M, Hidema J, Ishida H
  • Journal Title: Plant Signaling & Behavior Volume: 8 Issue: 8 Pages: 443-443
  • DOI: 10.4161/psb.25023
  • Peer Reviewed
• [Journal Article] Evidence for contribution of autophagy to Rubisco degradation during leaf senescence in Arabidopsis thaliana. (2013)
  • Author(s): Ono Y, Wada S, Izumi M, Makino A, Ishida H
  • Journal Title: Plant, Cell and Environment Volume: (In press) Issue: 6 Pages: 1147-1159
  • DOI: 10.1111/pce.12049
  • Peer Reviewed
• [Journal Article] Autophagy contributes to nighttime energy availability for growth in Arabidopsis. (2013)
  • Author(s): Izumi M, Hidema J, Makino A, Ishida H
  • Journal Title: Plant Physiology Volume: 161 Issue: 4 Pages: 1682-1693
  • DOI: 10.1104/pp.113.215632
  • Peer Reviewed
• [Presentation] 異なる窒素栄養条件下においてオートファジーの欠損がイネの窒素利用と成長に与える影響の解析 (2016)
  • Author(s): 横浜諒、和田慎也、菅野圭一、小島創一、山谷知行、牧野周、石田宏幸
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岩手大学（岩手県盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] イネの発芽過程におけるオートファジーの役割 (2015)
  • Author(s): 和田慎也、中村 萌、石田宏幸、牧野 周
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 京都大学（京都府京都市左京区）
  • Year and Date: 2015-09-09
• [Presentation] シロイヌナズナのRCBおよび葉緑体オートファジーにおけるATI（ATG8-interacting proteins）の役割について (2015)
  • Author(s): 石田宏幸、西村 翼、泉 正範、Gad Galili、牧野 周
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 京都大学（京都府京都市左京区）
  • Year and Date: 2015-09-09
• [Presentation] イネの栄養成長と老化葉の窒素リサイクルにおけるオートファジーの役割の解析 (2015)
  • Author(s): 和田慎也、林田泰和、泉 正範、来須孝光、花俣 繁、朽津和幸、牧野 周、石田宏幸
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 京都大学（京都府京都市左京区）
  • Year and Date: 2015-09-09
• [Presentation] シロイヌナズナにおけるオートファジーが亜鉛欠乏時に果たす役割 (2015)
  • Author(s): 江口雅丈、吉本光希、木村和彦、泉 正範、和田慎也、牧野 周、石田宏幸
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 京都大学（京都府京都市左京区）
  • Year and Date: 2015-09-09
• [Presentation] Establishment of monitoring methods for autophagy in rice reveals autophagic recycling of chloroplasts and root plastids during energy limitation (2015)
  • Author(s): Masanori Izumi，Jun Hidema，Shinya Wada，Eri Kondo，Takamitsu Kurusu，Kazuyuki Kuchitsu，Amane Makino，Hiroyuki Ishida
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京農業大学、世田谷
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 異なる窒素栄養条件下においてオートファジーの欠損がイネの窒素利用と成長に及ぼす影響の解析 (2015)
  • Author(s): 横浜諒，和田慎也，牧野周，石田宏幸
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京農業大学、世田谷
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] イネの栄養成長と老化葉の窒素リサイクルにおける オートファジーの役割の解析 (2015)
  • Author(s): 和田慎也，林田泰和, 泉正範，来須孝光，花俣繁，朽津和幸，牧野周，石田宏幸
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京農業大学、世田谷
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 異なる窒素栄養条件下においてオートファジーの欠損がイネの窒素利用と成長に及ぼす影響の解析 (2014)
  • Author(s): 和田慎也, 横浜諒, 石田宏幸, 牧野周
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 東京農工大学、府中
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] Autophagy for Photodamaged Chloroplast in Arabidopsis (2014)
  • Author(s): Izumi，M., Ishida，H., Makino，A., Hidema, J.
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山（富山大学）
• [Presentation] オートファジーの欠損がイネの栄養成長と窒素転流へ与える影響の解析 (2014)
  • Author(s): 林田泰和、和田慎也、来須孝光、朽津和幸、牧野周、石田宏幸
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山（富山大学）
• [Presentation] 植物の栄養リサイクルと葉緑体のオートファジー (2014)
  • Author(s): 石田宏幸
  • Organizer: 日本農芸化学会シンポジウム
  • Place of Presentation: 生田（明治大学）
  • Invited
• [Presentation] イネオートファジー欠損変異体Osatg7 の生理解析－ ( その１) 栄養成長期における個体生育について－ (2013)
  • Author(s): 和田慎也、林田泰和、来須孝光、朽津和幸、牧野周、石田宏幸
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋（名古屋大学）
• [Presentation] イネオートファジー欠損変異体Osatg7 の生理解析－（その２）葉の老化過程における窒素転流への影響について－ (2013)
  • Author(s): 林田泰和、和田慎也、来須孝光、朽津和幸、牧野周、石田宏幸
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋（名古屋大学）
• [Presentation] イネにおけるRubisco-containing body/ オートファジー系の可視化 (2013)
  • Author(s): 泉正範、日手間純、近藤依里、牧野周、石田宏幸
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋（名古屋大学）
• [Presentation] Role of autophagy in Rubisco degradation during leaf senescence (2013)
  • Author(s): Ishida, H., Ono, Y., Wada, S., Izumi, M., Yoshimoto, K., Mae, T., Makino, A.
  • Organizer: 6th European Workshop on Leaf Senescence
  • Place of Presentation: フランス（INRA-Versailles）
• [Presentation] Identification of a rice autophagy defected mutant, OsATG7, and phenotypes in the life cycle (2013)
  • Author(s): Wada, S., Hatyashida, Y., Kurusu, T., Kuchitsu, K., Makino, A., Ishida, H.
  • Organizer: 6th European Workshop on Leaf Senescence
  • Place of Presentation: フランス（INRA-Versailles）
• [Presentation] Autophagy in plant energy availability (2013)
  • Author(s): Izumi, M., Hirota, T., Hidema, J., Makino, A.
  • Organizer: 6th European Workshop on Leaf Senescence
  • Place of Presentation: フランス（INRA-Versailles）
• [Presentation] Autophagy contributes to Rubisco degradation during leaf senescence in Arabidopsis (2013)
  • Author(s): 小野佑樹、和田慎也、泉正範、牧野周 石田宏幸
  • Organizer: 第54回日本植物生理学会
  • Place of Presentation: 岡山大学(岡山市)
• [Presentation] シロイヌナズナのエネルギー利用におけるオートファジーの寄与について (2013)
  • Author(s): 泉正範、日出間純、牧野周、石田宏幸
  • Organizer: 第54回日本植物生理学会
  • Place of Presentation: 岡山大学(岡山市)
• [Presentation] Autophagy of chloroplasts during leaf senescence (2012)
  • Author(s): Ishida H.
  • Organizer: 10th International Congress on Plant Molecular Biology
  • Place of Presentation: 済州島ICC(韓国)(招待講演)
• [Presentation] シロイヌナズナ葉の栄養素リサイクルにおいてRCB/オートファジー系が担う役割の解析 (2012)
  • Author(s): 泉正範、日出間純、牧野周、石田宏幸
  • Organizer: 第58回日本土壌肥料学会
  • Place of Presentation: 鳥取大学(鳥取市)
• [Presentation] Rubisco分解におけるオートファジーの貢献度の評価 (2012)
  • Author(s): 小野佑樹、和田慎也、泉正範、牧野周、石田宏素
  • Organizer: 第58回日本土壌肥料学会
  • Place of Presentation: 鳥取大学(鳥取市)