Study of the avoidance mechanism of reactive oxygen species production based on a change of energy metabolism in plant

• Project/Area Number: 24380039
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Okayama University
• Principal Investigator: Yamamoto Yoko 岡山大学, 資源植物科学研究所, 教授 (50166831)
• Co-Investigator(Renkei-kenkyūsha): SASAKI Takayuki 岡山大学, 資源植物科学研究所, 助教 (60362985) ; TSUCHIYA Yoshiyuki 岡山大学, 資源植物科学研究所, 技術職員 (60720670)
• Research Collaborator: MUHAMMAD Sameeullah ; KARIYA Koki ; KOMATSU Kazue
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: アルミニウム耐性 / アルミニウム障害 / 液胞プロセシング酵素 / 活性酸素種 / 細胞死 / スクロース輸送体 / スクロースシンターゼ / タバコ培養細胞株 / アルミニウム応答 / インべルターゼ / エチレン合成関連遺伝子 / タバコ / ALMT1 / エネルギー代謝 / マイクロアレイ解析 / ミトコンドリア / ＶＰＥ / アルミニウム / アルコール発酵 / 好気呼吸 / 乳酸発酵

Outline of Final Research Achievements

A major inhibitory factor of crop production in acidic soils is aluminum (Al) ion. Using cultured tobacco cell lines and seedlings of tobacco, we found (1) a novel avoidance mechanism of ROS production in mitochondria by repression of TCA cycle but enhancement of fermentations in cytosol even under normoxia, which is similar to the Warburg effect reported in cancer cells, (2) an increase in Al tolerance by the over-expression of sucrose transporter in the plasma membrane, and (3) the involvement of vacuolar processing enzyme in cell death mechanism under Al stress.

Research Products

• [Journal Article] A chimeric protein of aluminum-activated malate transporter generated from wheat and Arabidopsis shows enhanced response to trivalent cations (2016)
  • Author(s): Sasaki T., Tsuchiya, Y., Ariyoshi, M., Ryan, P.R. and Yamamoto, Y.
  • Journal Title: Biochimica et Biophysica Acta Volume: 1858 Pages: 1427-1435
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A domain-based approach for analyzing the function of aluminum-activated malate transporters from wheat (Triticum aestivum) and Arabidopsis thaliana in Xenopus oocytes. (2014)
  • Author(s): Sasaki, T., Tsuchiya, Y., Ariyoshi, M., Ryan, P.R., Furuichi, T. and Yamamoto Y.
  • Journal Title: Plant Cell Physiol. Volume: 55 Issue: 12 Pages: 2126-2138
  • DOI: 10.1093/pcp/pcu143
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A novel mechanism of aluminium-induced cell death involving vacuolar processing enzyme and vacuolar collapse in tobacco cell line BY-2. (2013)
  • Author(s): Kariya K, Demiral T, Sasaki T, Tsuchiya Y, Turkan I, Sano T, Hasezawa S, Yamamoto Y.
  • Journal Title: J. Inorg. Biochem. Volume: 128 Pages: 196-201
  • Peer Reviewed
• [Journal Article] Sucrose transporter NtSUT1 confers aluminum tolerance on cultured cells of tobacco (Nicotiana tabacum L.). (2013)
  • Author(s): Muhammad S., Sasaki T., Yamamoto Y.
  • Journal Title: Soil Sci. Plant Nutri. Volume: 59 Issue: 5 Pages: 756-770
  • DOI: 10.1080/00380768.2013.830230
  • NAID: 110009766777
  • Peer Reviewed
• [Presentation] キメラALMT 輸送体の三価陽イオンへの応答促進 (2016)
  • Author(s): 佐々木孝行・土屋善幸・有吉美智代・Peter Ryan・山本洋子
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岩手大学（盛岡）
  • Year and Date: 2016-03-18
• [Presentation] Mechanisms of aluminum toxicity and tolerance elucidated by use of cultured tobacco cell lines. (2015)
  • Author(s): Yamamoto, Y., Sameeullah, M., Kariya K., Tsuchiya, Y. and Sasaki, T.
  • Organizer: 9th International Symposium on Plant-Soil Interactions at Low pH
  • Place of Presentation: Dubrovnik (Croatia)
  • Year and Date: 2015-10-18
  • Int'l Joint Research
• [Presentation] Transcriptomic analysis of aluminum-tolerant phenotype of a cultured cell line of tobacco (Nicotiana tabacum L.) (2015)
  • Author(s): Tsuchiya, Y., Kariya, K., Sasaki,T. and Yamamoto, Y.
  • Organizer: 9th International Symposium on Plant-Soil Interactions at Low pH
  • Place of Presentation: Dubrovnik (Croatia)
  • Year and Date: 2015-10-18
  • Int'l Joint Research
• [Presentation] An aluminum-induced cell death mechanism involving vacuolar processing enzyme in tobacco (2015)
  • Author(s): Kariya, K., Tsuchiya, Y., Sasaki, T. and Yamamoto, Y.
  • Organizer: 9th International Symposium on Plant-Soil Interactions at Low pH
  • Place of Presentation: Dubrovnik (Croatia)
  • Year and Date: 2015-10-18
  • Int'l Joint Research
• [Presentation] アルミニウム耐性タバコ培養細胞における高発現遺伝子群の解析 (2015)
  • Author(s): 土屋善幸・苅谷耕輝・佐々木孝行・山本洋子
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 京都大学（京都）
  • Year and Date: 2015-09-09
• [Presentation] 日本土壌肥料学会 (2015)
  • Author(s): 苅谷耕輝・土屋善幸・佐々木孝行・山本洋子
  • Organizer: アルミニウム耐性タバコ培養細胞株と野生株に発現する遺伝子群の比較解析．
  • Place of Presentation: 京都大学（京都）
  • Year and Date: 2015-09-09
• [Presentation] 植物特異的リンゴ酸輸送体ALMTの機能多様性の解析． (2015)
  • Author(s): 佐々木孝行・土屋善幸・有吉美智代・東泉恵美・山本洋子
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 京都大学（京都）
  • Year and Date: 2015-09-09
• [Presentation] タバコの培養細胞ならびに根におけるアルミニウムによる細胞死に伴うVPE遺伝子の発現誘導. (2015)
  • Author(s): 苅谷耕輝・土屋善幸・佐々木孝行・山本洋子
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] アルミニウタバコ培養細胞株と野生株に発現する遺伝子群の比較解析. (2015)
  • Author(s): 土屋善幸・苅谷耕輝・佐々木孝行・山本洋子
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Aluminum toxicity and tolerance mechanisms elucidated by using cultured cell and whole plant systems (2015)
  • Author(s): 山本洋子
  • Organizer: Seventh Symposium on Plant Stress Science Research
  • Place of Presentation: 倉敷
  • Year and Date: 2015-03-02 – 2015-03-03
  • Invited
• [Presentation] タバコのアルミニウムによる根生育阻害における液胞の関わり (2014)
  • Author(s): 苅谷耕輝・土屋善幸・佐々木孝行・山本洋子
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] タバコ培養細胞におけるアルミニウム耐性とスクロースシンターゼとの関係 (2014)
  • Author(s): 土屋善幸・苅谷耕輝・佐々木孝行・山本洋子
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] Auxin inducible sucrose transporter NtSUT1 is involved in aluminum tolerance in tobacco cells. (2013)
  • Author(s): Sameeullah, M., Sasaki, T., Yamamoto, Y.
  • Organizer: Plant Nutrition Colloquium
  • Place of Presentation: Istanbul, Turkey
• [Presentation] A novel mechanism of aluminum-induced cell death involving vacuole in tobacco cell line BY-2. (2013)
  • Author(s): Kariya, K., Demiral, T., Tsuchiya, Y., Sasaki, T., Yamamoto, Y.
  • Organizer: Plant Nutrition Colloquium
  • Place of Presentation: Istanbul, Turkey
• [Presentation] タバコ培養細胞を用いたAlによる細胞死誘発機構の解析 (2013)
  • Author(s): 土屋善幸・苅谷耕輝・山本洋子
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋
• [Presentation] タバコ培養細胞株BY-2におけるアルミニウムが誘発するVPEが関わる細胞死. (2013)
  • Author(s): 苅谷耕輝・土屋善幸・山本洋子
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋
• [Presentation] Aluminium-induced cell death process involves both mitochondria and vacuole in plant cells. (2013)
  • Author(s): Yoko Yamamoto, Koki Kariya, Yoshiyuki Tsuchiya, Tijen Demiral* and Takayuki Sasaki
  • Organizer: 10th Keel Meeting of Aluminium
  • Place of Presentation: Sutton Scotney, Winchester, England
• [Presentation] BY-2タバコ培養細胞をにおいてアルミニウムが誘発する細胞死における液胞のかかわり. (2013)
  • Author(s): 苅谷耕輝・佐々木孝行・山本洋子
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山
• [Presentation] Ｒｏｌｅ ｏｆ ｐｌａｓｍａ ｍｅｍｂｒａｎｅ ｓｕｃｒｏｓｅ ｔｒａｎｓｐｏｒｔｅｒ ｉｎ ｇｒｏｗｔｈ ａｎｄ ａｌｕｍｉｎｕｍ ｒｅｓｐｏｎｓｅｓ in tobacco cells. (2013)
  • Author(s): Sameeullah, M., Sasaki, T., Yamamoto, Y.
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山
• [Presentation] アルミニウム応答におけるエネルギー代謝の関わり:アルミニウム耐性の異なるタバコ培養細胞株の比較解析 (2012)
  • Author(s): 山本洋子・泉洋平・信濃卓郎・中村卓司・岡崎圭毅・佐々木孝行
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 鳥取
• [Presentation] Role of sucrose transporter (NtSUT1) under normal growth and Al stress conditions in cultured tobacco cells. (2012)
  • Author(s): Sameeullah, M., Sasaki, T., Yamamoto, Y.
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 鳥取
• [Presentation] Mechanisms of aluminum-induced cell death in plant cells. (2012)
  • Author(s): Yamamoto, Y., Demiral, T., Sasaki, T., Sano, T., Hasezawa, S., Izumi, Y.
  • Organizer: 8th International Symposium on Plant-Soil Interactions at Low pH
  • Place of Presentation: Bengaluru, India
• [Presentation] タバコ培養細胞BY-2を用いたアルミニウムによる細胞死の解析.
  • Author(s): 苅谷耕輝・佐々木孝行・山本洋子
  • Organizer: 日本土壌肥料学会 関西支部会
  • Place of Presentation: 倉敷
• [Book] Article title: Plant roots under aluminum stress. In: Plant Roots: The Hidden Half, Fourth Edition. Eds: Eshel Amram, Tom Beeckman, (2013)
  • Author(s): Matsumoto, H., Yammamoto, Y.
  • Publisher: CRC Press, Taylor & Francis Group, Boca Raton Florida
• [Remarks] 岡山大学資源植物科学研究所植物成長グループ
• [Remarks] 岡山大学資源植物科学研究所植物生長制御グループ
  • URL: http://www.rib.okayama-u.ac.jp/research/pg-hp.html
• [Remarks] 岡山大学資源植物科学研究所植物生長制御グループ
  • URL: http://www.rib.okayama-u.ac.jp/research/pg-hp.html