• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study of the avoidance mechanism of reactive oxygen species production based on a change of energy metabolism in plant

Research Project

Project/Area Number 24380039
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionOkayama University

Principal Investigator

Yamamoto Yoko  岡山大学, 資源植物科学研究所, 教授 (50166831)

Co-Investigator(Renkei-kenkyūsha) SASAKI Takayuki  岡山大学, 資源植物科学研究所, 助教 (60362985)
TSUCHIYA Yoshiyuki  岡山大学, 資源植物科学研究所, 技術職員 (60720670)
Research Collaborator MUHAMMAD Sameeullah  
KARIYA Koki  
KOMATSU Kazue  
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywordsアルミニウム耐性 / アルミニウム障害 / 液胞プロセシング酵素 / 活性酸素種 / 細胞死 / スクロース輸送体 / スクロースシンターゼ / タバコ培養細胞株 / アルミニウム応答 / インべルターゼ / エチレン合成関連遺伝子 / タバコ / ALMT1 / エネルギー代謝 / マイクロアレイ解析 / ミトコンドリア / VPE / アルミニウム / アルコール発酵 / 好気呼吸 / 乳酸発酵
Outline of Final Research Achievements

A major inhibitory factor of crop production in acidic soils is aluminum (Al) ion. Using cultured tobacco cell lines and seedlings of tobacco, we found (1) a novel avoidance mechanism of ROS production in mitochondria by repression of TCA cycle but enhancement of fermentations in cytosol even under normoxia, which is similar to the Warburg effect reported in cancer cells, (2) an increase in Al tolerance by the over-expression of sucrose transporter in the plasma membrane, and (3) the involvement of vacuolar processing enzyme in cell death mechanism under Al stress.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (31 results)

All 2016 2015 2014 2013 2012 Other

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Acknowledgement Compliant: 2 results) Presentation (23 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (1 results) Remarks (3 results)

  • [Journal Article] A chimeric protein of aluminum-activated malate transporter generated from wheat and Arabidopsis shows enhanced response to trivalent cations2016

    • Author(s)
      Sasaki T., Tsuchiya, Y., Ariyoshi, M., Ryan, P.R. and Yamamoto, Y.
    • Journal Title

      Biochimica et Biophysica Acta

      Volume: 1858 Pages: 1427-1435

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A domain-based approach for analyzing the function of aluminum-activated malate transporters from wheat (Triticum aestivum) and Arabidopsis thaliana in Xenopus oocytes.2014

    • Author(s)
      Sasaki, T., Tsuchiya, Y., Ariyoshi, M., Ryan, P.R., Furuichi, T. and Yamamoto Y.
    • Journal Title

      Plant Cell Physiol.

      Volume: 55 Issue: 12 Pages: 2126-2138

    • DOI

      10.1093/pcp/pcu143

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A novel mechanism of aluminium-induced cell death involving vacuolar processing enzyme and vacuolar collapse in tobacco cell line BY-2.2013

    • Author(s)
      Kariya K, Demiral T, Sasaki T, Tsuchiya Y, Turkan I, Sano T, Hasezawa S, Yamamoto Y.
    • Journal Title

      J. Inorg. Biochem.

      Volume: 128 Pages: 196-201

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sucrose transporter NtSUT1 confers aluminum tolerance on cultured cells of tobacco (Nicotiana tabacum L.).2013

    • Author(s)
      Muhammad S., Sasaki T., Yamamoto Y.
    • Journal Title

      Soil Sci. Plant Nutri.

      Volume: 59 Issue: 5 Pages: 756-770

    • DOI

      10.1080/00380768.2013.830230

    • NAID

      110009766777

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] キメラALMT 輸送体の三価陽イオンへの応答促進2016

    • Author(s)
      佐々木孝行・土屋善幸・有吉美智代・Peter Ryan・山本洋子
    • Organizer
      日本植物生理学会
    • Place of Presentation
      岩手大学(盛岡)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Mechanisms of aluminum toxicity and tolerance elucidated by use of cultured tobacco cell lines.2015

    • Author(s)
      Yamamoto, Y., Sameeullah, M., Kariya K., Tsuchiya, Y. and Sasaki, T.
    • Organizer
      9th International Symposium on Plant-Soil Interactions at Low pH
    • Place of Presentation
      Dubrovnik (Croatia)
    • Year and Date
      2015-10-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Transcriptomic analysis of aluminum-tolerant phenotype of a cultured cell line of tobacco (Nicotiana tabacum L.)2015

    • Author(s)
      Tsuchiya, Y., Kariya, K., Sasaki,T. and Yamamoto, Y.
    • Organizer
      9th International Symposium on Plant-Soil Interactions at Low pH
    • Place of Presentation
      Dubrovnik (Croatia)
    • Year and Date
      2015-10-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] An aluminum-induced cell death mechanism involving vacuolar processing enzyme in tobacco2015

    • Author(s)
      Kariya, K., Tsuchiya, Y., Sasaki, T. and Yamamoto, Y.
    • Organizer
      9th International Symposium on Plant-Soil Interactions at Low pH
    • Place of Presentation
      Dubrovnik (Croatia)
    • Year and Date
      2015-10-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] アルミニウム耐性タバコ培養細胞における高発現遺伝子群の解析2015

    • Author(s)
      土屋善幸・苅谷耕輝・佐々木孝行・山本洋子
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      京都大学(京都)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 日本土壌肥料学会2015

    • Author(s)
      苅谷耕輝・土屋善幸・佐々木孝行・山本洋子
    • Organizer
      アルミニウム耐性タバコ培養細胞株と野生株に発現する遺伝子群の比較解析.
    • Place of Presentation
      京都大学(京都)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 植物特異的リンゴ酸輸送体ALMTの機能多様性の解析.2015

    • Author(s)
      佐々木孝行・土屋善幸・有吉美智代・東泉恵美・山本洋子
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      京都大学(京都)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] タバコの培養細胞ならびに根におけるアルミニウムによる細胞死に伴うVPE遺伝子の発現誘導.2015

    • Author(s)
      苅谷耕輝・土屋善幸・佐々木孝行・山本洋子
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] アルミニウタバコ培養細胞株と野生株に発現する遺伝子群の比較解析.2015

    • Author(s)
      土屋善幸・苅谷耕輝・佐々木孝行・山本洋子
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Aluminum toxicity and tolerance mechanisms elucidated by using cultured cell and whole plant systems2015

    • Author(s)
      山本洋子
    • Organizer
      Seventh Symposium on Plant Stress Science Research
    • Place of Presentation
      倉敷
    • Year and Date
      2015-03-02 – 2015-03-03
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] タバコのアルミニウムによる根生育阻害における液胞の関わり2014

    • Author(s)
      苅谷耕輝・土屋善幸・佐々木孝行・山本洋子
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      東京
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] タバコ培養細胞におけるアルミニウム耐性とスクロースシンターゼとの関係2014

    • Author(s)
      土屋善幸・苅谷耕輝・佐々木孝行・山本洋子
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      東京
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] Auxin inducible sucrose transporter NtSUT1 is involved in aluminum tolerance in tobacco cells.2013

    • Author(s)
      Sameeullah, M., Sasaki, T., Yamamoto, Y.
    • Organizer
      Plant Nutrition Colloquium
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2013 Annual Research Report
  • [Presentation] A novel mechanism of aluminum-induced cell death involving vacuole in tobacco cell line BY-2.2013

    • Author(s)
      Kariya, K., Demiral, T., Tsuchiya, Y., Sasaki, T., Yamamoto, Y.
    • Organizer
      Plant Nutrition Colloquium
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2013 Annual Research Report
  • [Presentation] タバコ培養細胞を用いたAlによる細胞死誘発機構の解析2013

    • Author(s)
      土屋善幸・苅谷耕輝・山本洋子
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋
    • Related Report
      2013 Annual Research Report
  • [Presentation] タバコ培養細胞株BY-2におけるアルミニウムが誘発するVPEが関わる細胞死.2013

    • Author(s)
      苅谷耕輝・土屋善幸・山本洋子
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋
    • Related Report
      2013 Annual Research Report
  • [Presentation] Aluminium-induced cell death process involves both mitochondria and vacuole in plant cells.2013

    • Author(s)
      Yoko Yamamoto, Koki Kariya, Yoshiyuki Tsuchiya, Tijen Demiral* and Takayuki Sasaki
    • Organizer
      10th Keel Meeting of Aluminium
    • Place of Presentation
      Sutton Scotney, Winchester, England
    • Related Report
      2012 Annual Research Report
  • [Presentation] BY-2タバコ培養細胞をにおいてアルミニウムが誘発する細胞死における液胞のかかわり.2013

    • Author(s)
      苅谷耕輝・佐々木孝行・山本洋子
    • Organizer
      日本植物生理学会
    • Place of Presentation
      岡山
    • Related Report
      2012 Annual Research Report
  • [Presentation] Role of plasma membrane sucrose transporter in growth and aluminum responses in tobacco cells.2013

    • Author(s)
      Sameeullah, M., Sasaki, T., Yamamoto, Y.
    • Organizer
      日本植物生理学会
    • Place of Presentation
      岡山
    • Related Report
      2012 Annual Research Report
  • [Presentation] アルミニウム応答におけるエネルギー代謝の関わり:アルミニウム耐性の異なるタバコ培養細胞株の比較解析2012

    • Author(s)
      山本洋子・泉洋平・信濃卓郎・中村卓司・岡崎圭毅・佐々木孝行
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      鳥取
    • Related Report
      2012 Annual Research Report
  • [Presentation] Role of sucrose transporter (NtSUT1) under normal growth and Al stress conditions in cultured tobacco cells.2012

    • Author(s)
      Sameeullah, M., Sasaki, T., Yamamoto, Y.
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      鳥取
    • Related Report
      2012 Annual Research Report
  • [Presentation] Mechanisms of aluminum-induced cell death in plant cells.2012

    • Author(s)
      Yamamoto, Y., Demiral, T., Sasaki, T., Sano, T., Hasezawa, S., Izumi, Y.
    • Organizer
      8th International Symposium on Plant-Soil Interactions at Low pH
    • Place of Presentation
      Bengaluru, India
    • Related Report
      2012 Annual Research Report
  • [Presentation] タバコ培養細胞BY-2を用いたアルミニウムによる細胞死の解析.

    • Author(s)
      苅谷耕輝・佐々木孝行・山本洋子
    • Organizer
      日本土壌肥料学会 関西支部会
    • Place of Presentation
      倉敷
    • Related Report
      2012 Annual Research Report
  • [Book] Article title: Plant roots under aluminum stress. In: Plant Roots: The Hidden Half, Fourth Edition. Eds: Eshel Amram, Tom Beeckman,2013

    • Author(s)
      Matsumoto, H., Yammamoto, Y.
    • Publisher
      CRC Press, Taylor & Francis Group, Boca Raton Florida
    • Related Report
      2013 Annual Research Report
  • [Remarks] 岡山大学資源植物科学研究所植物成長グループ

    • Related Report
      2014 Annual Research Report
  • [Remarks] 岡山大学資源植物科学研究所植物生長制御グループ

    • URL

      http://www.rib.okayama-u.ac.jp/research/pg-hp.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 岡山大学資源植物科学研究所植物生長制御グループ

    • URL

      http://www.rib.okayama-u.ac.jp/research/pg-hp.html

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi