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Regulatory machinery of sulfur assimilation and metabolism in Arabidopsis: Repression of glucosinolates biosynthesis and induction of SULTR2;1 expression under sulfur deficient conditions.

Research Project

Project/Area Number 24380040
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionKyushu University

Principal Investigator

Maruyama-Nakashita Akiko  九州大学, (連合)農学研究科(研究院), 准教授 (70342855)

Project Period (FY) 2012-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Keywords硫黄同化 / 硫黄代謝 / タンパク質間相互作用 / SLIM1 / グルコシノレート / SDI / 転写制御 / ドメイン解析 / 硫酸イオン輸送 / 変異株
Outline of Final Research Achievements

Sulfur (S) is one of the essential macro elements and its assimilation and metabolism has a great impact on plant growth. In addition, there are many health-beneficial and stress-relief sulfur-containing compounds in plants and their contents are highly influenced by the S availability. In this study, some regulatory machinery of S assimilation and metabolism has been investigated. We found Sulfur deficiency induced (SDI)1 and 2 function as the repressors of glucosinolates (GSL) biosynthesis. We revealed that SDI1 binds to MYB28, a transcription factor stimulates the expression of GSL synthetic genes, in the nucleus and suppresses the expression of GSL biosynthetic genes. We also determined a sulfur responsive element SURE21 existing in the 3’untranscribed region of sulfate transporter SULTR2;1 and demonstrated its contribution to the induction of sulfate uptake and translocation to shoots. We also found cadmium treatment induces sulfate uptake and its translocation to shoots.

Report

(6 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (51 results)

All 2017 2016 2015 2014 2013 2012 Other

All Int'l Joint Research (6 results) Journal Article (8 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 8 results,  Open Access: 4 results,  Acknowledgement Compliant: 6 results) Presentation (28 results) (of which Int'l Joint Research: 7 results,  Invited: 3 results) Book (5 results) Remarks (4 results)

  • [Int'l Joint Research] Michigan State University(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] MaxPlank Institute/University of Koeln(ドイツ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] 忠南大学(韓国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Michigan State University(米国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Max-Plank Institute/University of Koeln(Germany)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] 忠南大学(韓国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 5&#x2032;-non-transcribed flanking region and 5&#x2032;-untranslated region play distinctive roles in sulfur deficiency induced expression of <i>SULFATE TRANSPORTER 1;2</i> in <i>Arabidopsis</i> roots2017

    • Author(s)
      *Maruyama-Nakashita A, Suyama A, Takahashi H
    • Journal Title

      Plant Biotechnology

      Volume: 34 Issue: 1 Pages: 51-55

    • DOI

      10.5511/plantbiotechnology.16.1226a

    • NAID

      130005519596

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Sulfur-deficiency-induced repressor proteins optimize glucosinolate biosynthesis in plants.2016

    • Author(s)
      Aarabi F, Kusajima M, Tohge T, Konishi T, Gigolashvili T, Takamune M, Sasazaki Y, Watanabe M, Nakashita H, Fernie A, Takahashi H, Saito K, Hubberten HM, Hoefgen R, *Maruyama-Nakashita A
    • Journal Title

      Science Advances

      Volume: 2 Issue: 10

    • DOI

      10.1126/sciadv.1601087

    • NAID

      120007008028

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Effects of cadmium treatment on the uptake and translocation of sulfate in Arabidopsis thaliana.2016

    • Author(s)
      Yamaguchi C, Takimoto Y, Ohkama-Ohtsu N, Hokura A, Shinano T, Nakamura T, Suyama A, *Maruyama-Nakashita A
    • Journal Title

      Plant Cell Physiology

      Volume: 57 Issue: 11 Pages: 2353-2366

    • DOI

      10.1093/pcp/pcw156

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Combinatorial use of sulfur-responsive regions of sulfate transporters provides a highly sensitive plant-based system for detecting selenate and chromate in the environment.2016

    • Author(s)
      *Maruyama-Nakashita A
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 62 Issue: 4 Pages: 386-391

    • DOI

      10.1080/00380768.2016.1152878

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Measurement of Uptake and Root-to-Shoot Distribution of Sulfate in Arabidopsis Seedlings.2016

    • Author(s)
      N. Yoshimoto, T. Kataoka, A. Maruyama-Nakashita, H. Takahashi
    • Journal Title

      Bio-protocol

      Volume: 6(1)

    • NAID

      120007008029

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Sulfur-responsive elements in the 3’-non-transcribed intergenic region are essential for the induction of SULFATE TRANSPORTER 2;1 gene expression in Arabidopsis roots under sulfur deficiency2015

    • Author(s)
      Maruyama-Nakashita A, Watanabe-Takahashi A, Inoue E, Yamaya T, Saito K Takahashi H
    • Journal Title

      The Plant Cell

      Volume: in press Issue: 4 Pages: 1279-1296

    • DOI

      10.1105/tpc.114.134908

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 植物細胞内の硫酸イオン輸送2015

    • Author(s)
      丸山明子
    • Journal Title

      RADIOISOTOPES

      Volume: 64 Pages: 549-551

    • NAID

      130005094738

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 植物の維管束を介した硫酸イオン輸送 -SULTR2;1の働きを中心に-2015

    • Author(s)
      丸山明子
    • Journal Title

      RADIOISOTOPES

      Volume: 64 Pages: 609-612

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] SDI1 inhibits aliphatic GSL biosynthesis through the interaction with MYB282017

    • Author(s)
      Maruyama-Nakashita A, Kusajima M, Gigolashvili T, Konishi T, Nakashita H
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学(鹿児島市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 植物の硫黄同化・代謝とその調節2016

    • Author(s)
      丸山明子
    • Organizer
      第89回日本生化学会大会フォーラム「イオウ原子が生み出す多彩な生体反応と含硫化合物の生理機能に関する最新知見」
    • Place of Presentation
      仙台国際センター(仙台市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 植物を用いた環境中セレン酸・クロム酸の検出系2016

    • Author(s)
      丸山明子
    • Organizer
      土壌肥料学会2016年度大会
    • Place of Presentation
      佐賀大学(佐賀市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] EIN3とのドメイン置換によるSLIM1機能ドメインの探索:エチレン応答か硫黄栄養応答か2016

    • Author(s)
      山口千仁、上土井優貴、丸山明子
    • Organizer
      土壌肥料学会2016年度大会
    • Place of Presentation
      佐賀大学(佐賀市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] ゼニゴケEILが硫黄栄養応答に果たす役割2016

    • Author(s)
      牧野宏美、前田祐華、上土井優貴、陶山明子、石崎公庸、石田咲子、西浜竜一、河内孝之、平山隆志、丸山明子
    • Organizer
      土壌肥料学会2016年度大会
    • Place of Presentation
      佐賀大学(佐賀市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Sulfur deficiency response in plants: Trade-off of the primary and secondary sulfur metabolism.2016

    • Author(s)
      Maruyama-Nakashita A
    • Organizer
      5th Sulphyton Workshop
    • Place of Presentation
      台北(台湾)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Detection and quantification of selenate and chromate using sulfur-responsive regions of sulfate transporters2016

    • Author(s)
      Maruyama-Nakashita A
    • Organizer
      5th Sulphyton Workshop
    • Place of Presentation
      台北(台湾)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of cadmium treatment on uptake and translocation of sulfate in Arabidopsis thaliana.2016

    • Author(s)
      Yamaguchi C, Takimoto Y, Hokura A, Ohkama-Ohtsu N, Suyama A, Shinano T, Maruyama-Nakashita A
    • Organizer
      5th Sulphyton Workshop
    • Place of Presentation
      台北(台湾)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 硫黄栄養の変化に応じた植物の硫黄同化と代謝の調節機構2016

    • Author(s)
      丸山明子
    • Organizer
      第二回 植物の栄養研究会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Sulfur-responsive elements in 3’-non-transcribed region of SULFATE TRANSPORTER 2;1 : Essential role in transcriptional regulation, sulfate uptake and translocation in Arabidopsis roots under sulfur-deficient conditions.2016

    • Author(s)
      Maruyama-Nakashita A, Watanabe-Takahashi A, Inoue E, Yamaya T, Saito K, Takahashi H
    • Organizer
      International Conference of Arabidopsis Research 2016
    • Place of Presentation
      慶州(大韓民国)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of cadmium treatment on uptake and translocation of sulfate in Arabidopsis thaliana.2016

    • Author(s)
      1)Yamaguchi C, Takimoto Y, Hokura A, Ohkama-Ohtsu N, Suyama A, Shinano T, Maruyama-Nakashita A
    • Organizer
      International Conference of Arabidopsis Research 2016
    • Place of Presentation
      慶州(大韓民国)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Detection and Quantification of Selenate and Chromate Using Sulfur-Responsive Regions of Sulfate Transporters2015

    • Author(s)
      A. Maruyama-Nakashita, Y. Takimoto, E. Inoue, K. Saito, H. Takahashi
    • Organizer
      ICOBTE2015
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2015-07-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of Cadmium Treatment on Uptake and Translocation of Sulfate in Arabidopsis thaliana2015

    • Author(s)
      C. Yamaguchi, Y. Takimoto, T. Shinano, A. Hokura, A. Maruyama-Nakashita
    • Organizer
      ICOBTE2015
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2015-07-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] SDIは含硫化合物グルコシノレートの生合成を抑制する2015

    • Author(s)
      丸山明子、高宗万希子、笹崎容子、峠隆之、斉藤和季、髙橋秀樹
    • Organizer
      第56 回日本植物生理学会年会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2015-03-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] ゼニゴケEILがエチレンおよび硫黄栄養応答に果たす役割2015

    • Author(s)
      牧野宏美、前田祐華、上土井優貴、陶山明子、石崎公庸、石田咲子、西浜竜一、河内孝之、平山隆志、丸山明子
    • Organizer
      第56 回日本植物生理学会年会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2015-03-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] 硫酸イオントランスポーターSULTR2;1の硫黄欠乏応答に働くシス配列 -遺伝子下流域による転写制御と地上部への硫酸イオン輸送に果たす役割-2015

    • Author(s)
      丸山 明子、髙橋 晶子、井上 恵理、山谷 知行、斉藤 和季、髙橋 秀樹
    • Organizer
      日本土壌肥料学会九州部会
    • Place of Presentation
      九州大学
    • Related Report
      2015 Annual Research Report
  • [Presentation] 硫黄栄養の応答欠損変異株の解析 -新規硫黄栄養応答制御因子の単離を目指して-2015

    • Author(s)
      山口千仁、中村有美子、髙橋秀樹、丸山明子
    • Organizer
      日本土壌肥料学会九州部会
    • Place of Presentation
      九州大学
    • Related Report
      2015 Annual Research Report
  • [Presentation] 硫黄栄養応答の転写制御機構2015

    • Author(s)
      丸山 明子
    • Organizer
      日本土壌肥料学会2015年京都大会
    • Place of Presentation
      京都大学
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] SULTR2;1遺伝子下流域の生理的な役割:硫酸イオン吸収と移行の促進にとどまらない何か2015

    • Author(s)
      丸山 明子
    • Organizer
      日本土壌肥料学会2015年京都大会
    • Place of Presentation
      京都大学
    • Related Report
      2015 Annual Research Report
  • [Presentation] カドミウム処理による植物体内の硫黄分配の変化2015

    • Author(s)
      山口千仁、陶山明子、瀧本裕希、大鎌直子、信濃卓郎、保倉明子、丸山明子
    • Organizer
      日本土壌肥料学会2015年京都大会
    • Place of Presentation
      京都大学
    • Related Report
      2015 Annual Research Report
  • [Presentation] Functional analysis of MpEIL in relation to ethylene and sulfur nutrition response2014

    • Author(s)
      Hiromi Makino, Yuka Maeda, Yuuki Jodoi, Akiko Suyama, Sakiko Ishida, Akiko Maruyama-Nakashita, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi
    • Organizer
      Marchantia Workshop 2014
    • Place of Presentation
      神戸大学
    • Year and Date
      2014-12-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] 硫黄二次代謝系の抑制因子RMGの発見2014

    • Author(s)
      丸山明子、高宗万希子、笹崎容子、峠隆之、濱野幸栄子、髙橋秀樹、斉藤和季
    • Organizer
      日本土壌肥料学会2014年度東京大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] A novel cis-acting element and transcription factor involved in the induction of SULTR1;2 expression and sulfate uptake activity under sulfur limitation in Arabidopsis roots.2014

    • Author(s)
      Akiko Maruyama-Nakashita, Makiko Takamune, Yuki Kimura, Yumiko Nakamura, Kazuki Saito, Hideki Takahashi
    • Organizer
      9th International Workshop Sulfur Metabolism in Plants
    • Place of Presentation
      Freiburg, Germany
    • Year and Date
      2014-04-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] 硫黄栄養の変化に応じた植物の硫黄同化と代謝の調節機構2014

    • Author(s)
      丸山明子
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 化合物処理により植物体内のグルタチオン量を増やす試み2013

    • Author(s)
      丸山明子
    • Organizer
      2013 年度日本土壌肥料学会九州支部春季例会
    • Place of Presentation
      佐賀大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 硫酸イオントランスポーターの硫黄欠乏による発現誘導を指標とした硫黄栄養応答欠損変異株の探索と表現型の解析2013

    • Author(s)
      山口千仁、林 純一、中村有美子、高橋秀樹、丸山明子
    • Organizer
      第8回トランスポーター研究会年会
    • Place of Presentation
      熊本大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 硫黄同化・代謝系の理解とその応用展開 -作物の質を支える元素として-2012

    • Author(s)
      丸山明子
    • Organizer
      日本土壌肥料学会2012年度大会
    • Place of Presentation
      鳥取大学(招待講演)
    • Year and Date
      2012-09-06
    • Related Report
      2012 Annual Research Report
  • [Presentation] 植物の硫酸イオン吸収・硫黄同化系制御機構の解明2012

    • Author(s)
      丸山明子
    • Organizer
      2012年度日本土壌肥料学会九州支部春期例会
    • Place of Presentation
      鹿児島大学(招待講演)
    • Year and Date
      2012-04-25
    • Related Report
      2012 Annual Research Report
  • [Book] Glutathione in plant growth, development and stress tolerance.2017

    • Author(s)
      *Maruyama-Nakashita A, Ohkama-Ohtsu N
    • Publisher
      Springer
    • Related Report
      2016 Annual Research Report
  • [Book] Plant Ecophysiology, Vol.11, Selenium in Plant.2017

    • Author(s)
      *Tamaoki M, Maruyama-Nakashita A
    • Publisher
      Springer
    • Related Report
      2016 Annual Research Report
  • [Book] Sulfur Metabolism in Higher Plants - Fundamental. Environmental and Agricultural Aspects.2017

    • Author(s)
      Yamaguchi C, *Maruyama-Nakashita A
    • Publisher
      Springer
    • Related Report
      2016 Annual Research Report
  • [Book] Sulfur Metabolism in Plants: Molecular Physiology and Ecophysiology2015

    • Author(s)
      L. J. De Kok, M. J. Hawkesford H. Rennenberg, K. Saito, E. Schnug
    • Total Pages
      300
    • Publisher
      Springer
    • Related Report
      2015 Annual Research Report
  • [Book] Sulfur Metabolism in Plants: Molecular Physiology and Ecophysiology2015

    • Author(s)
      Luit J. De Kok, Malcolm J. Hawkesford Heinz Rennenberg, Kazuki Saito, Ewald Schnug
    • Total Pages
      300
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Remarks] 植物が硫黄の不足に応じてグルコシノレートの生合成を止める仕組みを発見

    • URL

      http://www.kyushu-u.ac.jp

    • Related Report
      2016 Annual Research Report
  • [Remarks] 植物が栄養の少ない環境でも、栄養吸収を維持するしくみを発見

    • URL

      https://www.kyushu-u.ac.jp

    • Related Report
      2015 Annual Research Report
  • [Remarks] 植物を使って環境中の有害重金属を検出・定量するしくみの開発に成功!

    • Related Report
      2015 Annual Research Report
  • [Remarks]

    • URL

      http://bbs1.agr.kyusyu-u.ac.jp/prweb2/b06/amaru/amn/youkoso.html

    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2022-02-16  

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