| Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
|
|---|
| Outline of Final Research Achievements |
The filamentous fungus Aspergillus nidulans controls intracellular redox homeostasis by shuttling electron equivalents through cell membrane. Cellular NAD+/NADH level maintains the homeostasis and various fungal cell functions. This study employs A. nidulans as a model fungus, and revealed novel controlling mechanism of cellular redox state. I revealed that the Nudix hydrolase AnNUDT1 (NdxA) is a key controlling protein of cellular redox state. NdxA hydrolyzes NAD+/NADH, which affects rates of cellular thiamine biosynthesis and glycolysis, both of which use NAD+ as a substrate. NAD+ level also affects function of NAD+-dependent histone deacetylase isozyme (sirtuin A), and histone acetylation level, and hence fungal secondary metabolisms. These results shed light novel NdxA and NAD+/NADH function in the cellular processes, and contribute improved use of filamentous fungi in industry.
|
