Mode of functions of plasmid as the modulator of host chromosomal functions

• Project/Area Number: 24380043
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: The University of Tokyo
• Principal Investigator: NOJIRI Hideaki 東京大学, 生物生産工学研究センター, 教授 (90272468)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
• Keywords: プラスミド / 染色体 / 遺伝子発現 / 転写制御 / 核様体 / 転写調節 / 転写様式 / 核様体タンパク質 / 接合伝達

Outline of Final Research Achievements

To elucidate the modulation mechanism of host chromosomal functions by plasmid, the reduction of osmotic stress tolerance and the induction/reduction of the specific genes by plasmid carriage were investigated. In addition, mechanisms of dimerization/multimerization of plasmid-borne H-NS family proteins were investigated by protein-protein cross-linking assays and protein structure determined by X-ray crystallography.

Research Products

• [Journal Article] Effects of three different nucleoid-associated proteins encoded on IncP-7 plasmid pCAR1 on host Pseudomonas putida KT2440 (2015)
  • Author(s): Suzuki-Minakuchi C., R. Hirotani, M. Shintani, T. Takeda, Y. Takahashi, K. Matsui, D. Vasileva, C.-S. Yun, K. Okada, H. Yamane, and H. Nojiri
  • Journal Title: Applied and Environmental Microbiology Volume: 81 Issue: 8 Pages: 2869-2880
  • DOI: 10.1128/aem.00023-15
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Modulation of primary cell functions of host Pseudomonas bacteria by conjugative plasmid pCAR1 (2015)
  • Author(s): Takahashi, Y., M. Shintani, N. Takase, Y. Kazou, F. Kawamura, H. Hara, H. Nishida, K. Okada, H. Yamane, and H. Nojiri
  • Journal Title: Environmental Microbiology Volume: 17 Issue: 1 Pages: 134-155
  • DOI: 10.1111/1462-2920.12515
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Oligmerization mechanisms of an H-NS family protein, Pmr, encoded on the plasmid pCAR1 provide a molecular basis for functions of H-NS family members (2014)
  • Author(s): Suzuki, C., K. Kawazuma, S. Horita, T. Terada, M. Tanokura, K. Okada, H. Yamane, and H. Nojiri
  • Journal Title: PLoS ONE Volume: 9 Issue: 8 Pages: e105656-e105656
  • DOI: 10.1371/journal.pone.0105656
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 環境中におけるプラスミドの挙動解析 (2013)
  • Author(s): 新谷政己、松井一泰、金原和秀、野尻秀昭
  • Journal Title: Journal of Environmental Biotechnology Volume: 13 Pages: 125-134
  • Peer Reviewed
• [Journal Article] Impact of catabolic plasmids on host cell physiology (2013)
  • Author(s): Hideaki Nojiri
  • Journal Title: Current Opinion in Biotechnology Volume: (印刷中) Issue: 3 Pages: 423-430
  • DOI: 10.1016/j.copbio.2012.09.014
  • Peer Reviewed
• [Journal Article] 異なる栄養条件下におけるプラスミドの接合伝達頻度の比較解析 (2012)
  • Author(s): 新谷、野尻, ら(他4名)
  • Journal Title: Journal of Environmental Biotechnology Volume: 12 Pages: 163-167
  • Peer Reviewed
• [Journal Article] プラスミド保持に伴う負荷を軽減化する宿主染色体因子 (2012)
  • Author(s): 能登、野尻, ら(他9名)
  • Journal Title: Journal of Environmental Biotechnology Volume: 12 Pages: 135-139
• [Presentation] 混合バイオフィルム中でのプラスミドの挙動 (2015)
  • Author(s): 李昇昱、水口（鈴木）千穂、清川達則、野村暢彦、岡田憲典、野尻秀昭
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学津島キャンパス、岡山
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] プラスミドpCAR1上の転写開始点とプロモーター領域の網羅的解析 (2015)
  • Author(s): 舘はる香、水口（鈴木）千穂、高橋裕里香、大坪嘉行、津田雅孝、岡田憲典、野尻秀昭
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学津島キャンパス、岡山
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] プラスミドと宿主染色体由来MvaT様因子のヘテロ二量体形成機構 (2015)
  • Author(s): 川妻孝平、水口（鈴木）千穂、寺田透、藤本瑞、岡田憲典、野尻秀昭
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学津島キャンパス、岡山
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] Plasmid business: effects on the host cell physiology and plasmid fitness cost (2014)
  • Author(s): Hideaki Nojiri
  • Organizer: International Society for Plasmid Biology Scientific Meeting 2014
  • Place of Presentation: Palm Cove, Australia
  • Year and Date: 2014-10-27 – 2014-11-01
  • Invited
• [Presentation] プラスミドpCAR1にコードされる核様体タンパク質が宿主に及ぼす影響の解析 (2014)
  • Author(s): 水口（鈴木） 千穂、廣谷龍輔、高橋裕里香、松井一泰、武田俊春、尹忠銖、新谷政己、岡田憲典、山根久和、野尻秀昭
  • Organizer: 環境微生物系学会合同大会2014
  • Place of Presentation: 浜松アクトシティコングレスセンター、浜松
  • Year and Date: 2014-10-21 – 2014-10-24
• [Presentation] プラスミドpCAR1上の転写開始点とプロモーター領域の網羅的解析 (2014)
  • Author(s): 舘はる香、高橋裕里香、大坪嘉行、津田雅孝、水口（鈴木）千穂、岡田憲典、野尻秀昭
  • Organizer: 環境微生物系学会合同大会2014
  • Place of Presentation: 浜松アクトシティコングレスセンター、浜松
  • Year and Date: 2014-10-21 – 2014-10-24
• [Presentation] Bacterial Evolution in Environment via Plasmid Conjugation (2014)
  • Author(s): Hideaki Nojiri
  • Organizer: 環境微生物系学会合同大会2014
  • Place of Presentation: 浜松アクトシティコングレスセンター、浜松
  • Year and Date: 2014-10-21 – 2014-10-24
  • Invited
• [Presentation] プラスミドpCAR1上の転写開始点とプロモーター領域の網羅的解析 (2014)
  • Author(s): 舘はる香、水口（鈴木）千穂、高橋裕里香、大坪嘉行、津田雅孝、岡田憲典、野尻秀昭
  • Organizer: 日本農芸化学会関東支部2014年度支部大会
  • Place of Presentation: 埼玉大学、埼玉
  • Year and Date: 2014-10-18
• [Presentation] プラスミドと宿主染色体にコードされるMvaTホモログのホモ多量体・ヘテロ二量体形成機構の解明 (2014)
  • Author(s): 川妻 孝平, 水口（鈴木） 千穂, 岡田 憲典, 野尻 秀昭
  • Organizer: 第13回微生物研究会
  • Place of Presentation: 東京農業大学、東京
  • Year and Date: 2014-07-26
• [Presentation] Degradative Plasmids as the Agents for Bacterial Evolution (2014)
  • Author(s): Hideaki Nojiri
  • Organizer: Thailand-Japan Collaboration Symposium on Microbial Resources for Environmental and Industrial Applications
  • Place of Presentation: Chulalongkorn University, Bangkok, Thailand
  • Year and Date: 2014-06-03
  • Invited
• [Presentation] Cooperative Function of Plasmid- and Chromosomally Encoded H-NS Family Proteins (2014)
  • Author(s): Chiho Suzuki-Minakuchi
  • Organizer: Thailand-Japan Collaboration Symposium on Microbial Resources for Environmental and Industrial Applications
  • Place of Presentation: Chulalongkorn University, Bangkok, Thailand
  • Year and Date: 2014-06-03
  • Invited
• [Presentation] Plasmid business: plasmid effect to host cell physiology and fitness (2014)
  • Author(s): Hideaki Nojiri
  • Organizer: Mini-Symposium: Plasmid effect to host cell physiology and fitness
  • Place of Presentation: University of Massachusetts Amherst
  • Year and Date: 2014-05-21
  • Invited
• [Presentation] Modulation of primary cell function of host Pseudomonas bacteria by the conjugative plasmid pCAR1 (2014)
  • Author(s): Yurika Takahashi
  • Organizer: Mini-Symposium: Plasmid effect to host cell physiology and fitness
  • Place of Presentation: University of Massachusetts Amherst
  • Year and Date: 2014-05-21
• [Presentation] A key global regulator that alters host function: H-NS family protein encoded on the plasmid pCAR1 (2014)
  • Author(s): Chiho Suzuki-Minakuchi and Kohei Kawazuma
  • Organizer: Mini-Symposium: Plasmid effect to host cell physiology and fitness
  • Place of Presentation: University of Massachusetts Amherst
  • Year and Date: 2014-05-21
• [Presentation] Cooperative Function of the Three Nucleoid-Associated Proteins Encoded on IncP-7 Catabolic Plasmid pCAR1 (2014)
  • Author(s): Chiho Suzuki, Ryusuke Hirotani, Yurika Takahashi, Kazuhiro Matsui, Toshiharu Takeda, Choong-Soo Yun, Masaki Shintani, Kazunori Okada, Hisakazu Yamane, Hideaki Nojiri
  • Organizer: American Society for Microbiology 114th General Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2014-05-17 – 2014-05-20
• [Presentation] Homo-Oligomerization Manners of H-NS Family Proteins, IncP-7 Catabolic Plasmid pCAR1-BornePmr and its Pseudomonas Host Chromosome-Brone TurA and TurB (2014)
  • Author(s): K. Kawazuma, C. Suzuki, T. Terada, K. Okada, H. Yamane, H. Nojiri
  • Organizer: American Society for Microbiology 114th General Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2014-05-17 – 2014-05-20
• [Presentation] Comparisons of the Impacts on Host Cell by Carriage of a Different Plasmid (2014)
  • Author(s): Y. Takahashi, D. Sugiyama, M. Shintani, H. Yamane, K. Okada, M. Tsuda, H. Nojiri
  • Organizer: American Society for Microbiology 114th General Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2014-05-17 – 2014-05-20
• [Presentation] プラスミドpCAR1由来の3種の核様体タンパク質は協調的に機能する (2014)
  • Author(s): 鈴木千穂、廣谷龍輔、高橋裕里香、松井一泰、武田俊春、尹忠銖、新谷政己、岡田憲典、山根久和、野尻秀昭
  • Organizer: 日本ゲノム微生物学会
  • Place of Presentation: 東京
• [Presentation] プラスミドと宿主染色体にコードされるMvaTホモログのホモ多量体・ヘテロ二量体形成機構 (2014)
  • Author(s): 川妻孝平、鈴木千穂、藤本瑞、岡田憲典、野尻秀昭
  • Organizer: 日本ゲノム微生物学会
  • Place of Presentation: 東京
• [Presentation] プラスミドの保持が宿主細菌に与える影響のプラスミド間比較 (2014)
  • Author(s): 高橋裕里香、杉山大介、新谷政己、山根久和、岡田憲典、津田雅孝、野尻秀昭
  • Organizer: 日本ゲノム微生物学会
  • Place of Presentation: 東京
• [Presentation] プラスミド上及び宿主染色体にコードされるMvaTホモログ群の改変ChIP-chip解析 (2014)
  • Author(s): 高橋裕里香、尹忠銖、鈴木千穂、新谷政己、山根久和、岡田憲典、野尻秀昭
  • Organizer: 日本農芸化学会
  • Place of Presentation: 川崎
• [Presentation] pCAR1にコードされる核様体タンパク質の転写様式と相互作用の解析 (2014)
  • Author(s): 鈴木千穂、高橋裕里香、新谷政己、岡田憲典、山根久和、野尻秀昭
  • Organizer: 日本農芸化学会
  • Place of Presentation: 川崎
• [Presentation] プラスミドと宿主染色体にコードされるMvaTホモログのヘテロ二量体形成機構の解析 (2014)
  • Author(s): 川妻孝平、鈴木千穂、藤本瑞、岡田憲典、野尻秀昭
  • Organizer: 日本農芸化学会
  • Place of Presentation: 川崎
• [Presentation] 環境細菌の振る舞いと進化における接合伝達性プラスミドの役割 (2013)
  • Author(s): 野尻秀昭
  • Organizer: 日本遺伝学会
  • Place of Presentation: 横浜
• [Presentation] プラスミドを保持した細菌の応答のプラスミド間比較 (2013)
  • Author(s): 高橋裕里香、杉山大介、新谷政己、山根久和、岡田憲典、津田雅孝、野尻秀昭
  • Organizer: 極限環境生物学会
  • Place of Presentation: 川崎
• [Presentation] 難分解性環境汚染物質の分解細菌が有する分解能の分子基盤の解明 (2013)
  • Author(s): 野尻秀昭
  • Organizer: 極限環境生物学会
  • Place of Presentation: 川崎
  • Invited
• [Presentation] プラスミドと宿主染色体由来のH-NS槎因子による異なる転写制御機構の解析 (2013)
  • Author(s): 鈴木、野尻, ら (他6名)
  • Organizer: 第7回日本ゲノム微生物学会年会
  • Place of Presentation: 長浜バイオ大学(滋賀)
• [Presentation] バイオフィルム表現型の変化に関与する新規遺伝因子の探索 (2013)
  • Author(s): 李、野尻, ら(他4名)
  • Organizer: 第7回日本ゲノム微生物学会年会
  • Place of Presentation: 長浜バイオ大学(滋賀)
• [Presentation] プラスミドと宿主染色体にコードされるH-NS様因子が異なる機能を持つ機構の解析 (2013)
  • Author(s): 鈴木、野尻, ら (他6名)
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学川内芯北キャンパス(宮城)
• [Presentation] プラスミド保持細菌のプラスミドに対する応答の比較 (2013)
  • Author(s): 高橋、野尻, ら(他4名)
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学川内北キャンパス(宮城)
• [Presentation] バイオフィルム表現型の変化に関与する新規遺伝因子の探索 (2013)
  • Author(s): 李、野尻, ら(他5名)
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学川内北キャンパス(宮城)
• [Presentation] 宿主機能を制御する可動性遺伝因子プラスミドの働き (2012)
  • Author(s): 野尻秀昭
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場(福岡)(招待講演)
  • Year and Date: 2012-12-13
• [Presentation] プラスミドと宿主染色体にコードされるH-NS様因子が異なる機能を持つメカニズムの解析 (2012)
  • Author(s): 鈴木、野尻, ら(他5名)
  • Organizer: BIA Symposium 2012
  • Place of Presentation: 明治記念館(東京)
  • Year and Date: 2012-07-20
• [Presentation] ROLE OF THE H-NS FAMILY PROTEINS IN COOPERATIVE FUNCTION OF CARBAZOLE DEGRADATIVE PLASMID pCARl AND HOST CHROMOSOME (2012)
  • Author(s): Suzuki, Nojiri, ら(他5名)
  • Organizer: Environmental Microbiology and Biotechnology conference 2012
  • Place of Presentation: Bologna, Italy
• [Presentation] INTERACTION BETWEEN DEGRADATIVE PIASMID AND HOST CHROMOSOME DIFFER BETWEEN THREE PSEUDOMONAS HOSTS (2012)
  • Author(s): Takahashi, Nojiri, ら(他7名)
  • Organizer: Environmental Microbiology and Biotechnology Conference 2012
  • Place of Presentation: Bologna, Italy
• [Presentation] Plasmid, a key determinant of the fate of xenobiotic-degrading bacteria in natural eco-system (2012)
  • Author(s): Hideaki Nojiri
  • Organizer: Japan-Finland Biotechnology Symposium 2012
  • Place of Presentation: 東北学院大学押川記念ホール(宮城)(招待講演)
• [Presentation] Modulation of primary host cell function by conjugative plasmid pCARl (2012)
  • Author(s): Takahashi, Nojiri, ら(他6名)
  • Organizer: International Plasmid Biology Conference 2012
  • Place of Presentation: Santander, Spain
• [Presentation] Interaction between IncP-7 carbazole-degradative plasmid pCARl and host chromosome through H-NS family proteins (2012)
  • Author(s): Suzuki, Nojiri, ら(他6名)
  • Organizer: International Plasmid Biology Conference 2012
  • Place of Presentation: Santander, Spain
• [Presentation] Effects of carbazole degradative plasmid pCARI on biofilm formation of three Pseudomonas strains (2012)
  • Author(s): Lee, Nojiri, ら(他2名)
  • Organizer: 15th International Biotechnology Symposium and Exhibition
  • Place of Presentation: Daegu, Korea
• [Presentation] 分解プラスミドを保持した宿主の変化の網羅的解析 (2012)
  • Author(s): 高橋、野尻, ら(他6名)
  • Organizer: 日本農芸化学会関東支部2012年度大会
  • Place of Presentation: 新潟薬科大学(新潟)
• [Presentation] 染色体機能調節因子としてのプラスミドの機能の解明 (2012)
  • Author(s): 高橋、野尻, ら(他5名)
  • Organizer: 極限環境生物学会2012年度 (第13回) 年会
  • Place of Presentation: 日本大学文理学部(東京)
• [Presentation] Molecular Bases of Bacterial Carbazole Degradation
  • Author(s): Nojiri, H.
  • Organizer: Gadjah Mada University Seminar
  • Place of Presentation: Yogyakarta, Indonesia
  • Invited
• [Book] Biodegradative Bacteria (2013)
  • Author(s): Nojiri, H., M. Tsuda, M. Fukuda, Y. Kamagata
  • Total Pages: 358
  • Publisher: Springer