GENETIC STUDY ON BACTERIAL COLONY FORMATION USING A COLONIZATION-DEFECTIVE MUTANT
| Project/Area Number |
24380044
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MASAKI HARUHIKO 東京大学, 農学生命科学研究科, 教授 (50134515)
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| Co-Investigator(Kenkyū-buntansha) |
SHIGEMATSU Toru 新潟薬科大学, 応用生命科学部, 教授 (10315286)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Keywords | バクテリア / 大腸菌 / 温度感受性変異 / コロニー / 増殖 / 固体培養 / 液体培養 / 不飽和脂肪酸 / 脂肪酸合成 / セルレニン / オレイン酸 / VBNC / 液体培地 / 固体培地 / 寒天培地 / ゲランガム / 好気呼吸 / 電子伝達系 / 難培養細菌 / VNC / コロニー形成 |
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| Outline of Final Research Achievements |
Colony formation is a basic method for bacterial isolation and counting. But most natural live bacteria cannot be cultured due to low colony formation. Obviously being alive does not mean being able to form colonies, however, the difference is little understood in terms of gene expression. To gain a genetic insight into colony formation, we isolated a colonization-defective Escherichia coli mutant. The mutant was unable to synthesize unsaturated fatty acids. This mutant requires oleic acid for growth, but with a limited supply of oleic acid, its growth on solid medium was more sensitively and seriously impaired than in liquid medium. Enxymatical inhibition of fatty acid synthesis by cerulenin also impaired growth more seriously in solid cultures than liquid cultures of both wild-type E. coli and Bacullus subtilis. The depletion of fatty acids in the environment is probably one of the causes of low colony formation, which can be improved by fatty acid supplementation.
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Report
(5 results)
Research Products
(19 results)
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[Journal Article] Transfer-messenger RNA and SmpB mediate bacteriostasis in Escherichia coli cells against tRNA cleavage.2015
Author(s)
Sakai, F., Sugita,R., Chang, J.-W., Ogawa, T., Hidaka, M., Masaki, H.
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Journal Title
Microbiology
Volume: 161
Issue: 10
Pages: 2019-2028
DOI
Related Report
Peer Reviewed
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[Journal Article] Transition of serine residues to the D-form during the conversion of ovalbumin into heat stable S-ovalbumin.2015
Author(s)
Miyamoto, T., Takahashi, N., Sekine, M., Ogawa, T., Hidaka, M., Homma, H., Masaki, H.
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Journal Title
J. Pharm. Biomed. Anal.
Volume: 116
Pages: 145-149
DOI
Related Report
Peer Reviewed
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[Journal Article] Origin of D-amino acids detected in the acid hydrolysates of purified Escherichia coli ß-galactosidase.2015
Author(s)
Miyamoto, T., Sekine, M., Ogawa, T., Hidaka, M., Homma, H., Masaki, H.
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Journal Title
J. Pharm. Biomed. Anal.
Volume: 116
Pages: 105-108
DOI
Related Report
Peer Reviewed
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[Journal Article] Evidence for DNA cleavage caused by a transfer-tRNA-targeting toxin.2013
Author(s)
Shigematsu, M., Ogawa, T., Tanaka, W., Takahashi, K., Kitamoto, H.K., Hidaka, M, and Masaki, H,
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Journal Title
PLoS ONE
Volume: 8
Issue: 9
Pages: 75512-75512
DOI
Related Report
Peer Reviewed
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[Journal Article] Importance ofcell-damage causing growth delay for high-pressure inactivation of Saccharomyces cerevisiae2013
Author(s)
Masaru Nanba, Kazuki Nomura, Yusuke Nasuhara, Manabu Hayashi, Miyuki Kido, Mayumi Hayashi, Akinori Iguchi, Toru Shigematsu, Masao Hirayama, Shigeaki Ueno, Tomoyuki Fujii
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Journal Title
High Pressure Research
Volume: 33
Issue: 2
Pages: 299-307
DOI
Related Report
Peer Reviewed
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