| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Outline of Final Research Achievements |
Colony formation is a basic method for bacterial isolation and counting. But most natural live bacteria cannot be cultured due to low colony formation. Obviously being alive does not mean being able to form colonies, however, the difference is little understood in terms of gene expression. To gain a genetic insight into colony formation, we isolated a colonization-defective Escherichia coli mutant. The mutant was unable to synthesize unsaturated fatty acids. This mutant requires oleic acid for growth, but with a limited supply of oleic acid, its growth on solid medium was more sensitively and seriously impaired than in liquid medium. Enxymatical inhibition of fatty acid synthesis by cerulenin also impaired growth more seriously in solid cultures than liquid cultures of both wild-type E. coli and Bacullus subtilis. The depletion of fatty acids in the environment is probably one of the causes of low colony formation, which can be improved by fatty acid supplementation.
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