| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
We analyzed the function of 1-acyl-sn-glycerol-3-phosphate O-acyltransferase isozymes, PlsC1 and PlsC4, from Shewanella livingstonensis Ac10 and found that these enzymes are responsible for the synthesis of phospholipids containing eicosapentaenoic acid (EPA) and branched-chain fatty acids, respectively. PlsC1 was mainly localized at the cell division site, whereas PlsC4 was mainly localized at the polar regions of the cell. Thus these enzymes probably contribute to the biogenesis of heterogeneous cell membrane of this bacterium. We found that EPA-containing phospholipids facilitate folding of a membrane protein and synthesized a probe molecule useful for the analysis of the physiological functions of EPA.
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