• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Sustainable control of bacterial wilt by jumbo phage technology

Research Project

Project/Area Number 24380049
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionHiroshima University

Principal Investigator

YAMADA Takashi  広島大学, 先端物質科学研究科, 教授 (40230461)

Co-Investigator(Kenkyū-buntansha) FUJIE Makoto  広島大学, 大学院先端物質科学研究科, 准教授 (20274110)
KAWASAKI Takeru  広島大学, 大学院先端物質科学研究科, 助教 (00510299)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2012: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Keywordsbacteriophage / bacterial wilt / Ralstonia solanacearum / biocontrol / resistance cost / prophage induction / growth inhibition / loss of virulence / 青枯病 / バクテリオファージ / ジャンボファージ / バイオコントロール
Outline of Final Research Achievements

Four major mechanisms have been found to effects host virulence after infection by RSL1: (1) twitching motility and associated virulence were affected in the host cells,(2) among 12 RSL1 genes enhanced during prolonged infection, orf105, orf106, and orf121 worked for growth activation but orf137 and orf209 strongly inhibited the growth, (3) RSL1 induced RSS prophages in the host cells, and (4) phage resistance working in the host was associated with LPS structure and function, so that resistant cells all lost virulence because of LPS deficiency.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (30 results)

All 2014 2013 2012 Other

All Journal Article (12 results) (of which Acknowledgement Compliant: 3 results,  Peer Reviewed: 9 results,  Open Access: 3 results) Presentation (15 results) (of which Invited: 1 results) Book (1 results) Remarks (2 results)

  • [Journal Article] 青枯病菌の繊維状ファージ:病原性の諸刃の剣2014

    • Author(s)
      山田 隆
    • Journal Title

      植物細菌病談話会論文集

      Volume: 26 Pages: 46-53

    • Related Report
      2014 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] ファージを利用し他青枯病防除技術の開発:巨大ファージRSL1と繊維状ファージRSMsの有効性2014

    • Author(s)
      山田 隆
    • Journal Title

      化学と生物

      Volume: 52 Pages: 371-379

    • Related Report
      2014 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] The filamentous phage XacF1 causes loss of virulence in Xanthomonas axonopodis pv. citri, the causative agent of citrus canker disease.2014

    • Author(s)
      Ahmad, A.A., Askora, A., Kawasaki,T., Fujie, M., and Yamada,T.
    • Journal Title

      Front. Microbiol.

      Volume: 5 Pages: 3211-11

    • DOI

      10.3389/fmicb.2014.00321

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Insights into the diversity of φRSM phages infecting strains of the phytopathogen Ralstonia solanacearum complex : regulation and evolution2014

    • Author(s)
      Ahmed Askora, Takeru Kawasaki, Makoto Fujie and Takashi Yamada
    • Journal Title

      Mol. Genet. Genom.

      Volume: 289(in press) Issue: 4 Pages: 589-598

    • DOI

      10.1007/s00438-014-0835-3

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of Bacteriophages Cp1 and Cp2, the Strain-Typing Agents for Xanthomonas axonopodis pv. citri2014

    • Author(s)
      Abdelmonim Ali Ahmad, Megumi Ogawa, Takeru Kawasaki, Makoto Fujie and Takashi Yamada
    • Journal Title

      Appl. Environ. Microbiol.

      Volume: 80 Issue: 1 Pages: 77-85

    • DOI

      10.1128/aem.02310-13

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] バクテリオファージを用いた植物病診断・防除システムの開発2014

    • Author(s)
      山田 隆
    • Journal Title

      化学と生物

      Volume: 52

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Filamentous phages of Ralstonia solanacearum: Double-edged swords for pathogenic bacteria2013

    • Author(s)
      Yamada, T.
    • Journal Title

      Front. Microbiol.

      Volume: 4

    • DOI

      10.3389/fmicb.2013.00325

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cryo-electron microscopy 3D structure of the jumbo phage RSL1 infecting the phytopathogen2013

    • Author(s)
      Effantin, G., R. Hamasaki, T. Kawasaki, M. Bacia, T. Yamada and G. Schoehn
    • Journal Title

      Ralstonia solanacearum Structure

      Volume: 21(2) Issue: 2 Pages: 298-305

    • DOI

      10.1016/j.str.2012.12.017

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Site-specific recombination systems in filamentous phages.2012

    • Author(s)
      Askora, A., Abdel-Haliem. M.E.F., and Yamada, T.
    • Journal Title

      Mol. Genet. Genom.

      Volume: 287 Issue: 7 Pages: 525-530

    • DOI

      10.1007/s00438-012-0700-1

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Utilization of Filamentous Phage φRSM3 to Control Bacterial Wilt Caused by Ralstonia solanacearum2012

    • Author(s)
      Hardian S. Addy, Ahmed Askora, Takeru Kawasaki, Makoto Fujie, and Takashi Yamada
    • Journal Title

      Plant Disease

      Volume: 96 Issue: 8 Pages: 1204-1209

    • DOI

      10.1094/pdis-12-11-1023-re

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Loss of Virulence of the Phytopathogen Ralstonia solanacearum Through Infection byφRSM Filamentous Phages2012

    • Author(s)
      Hardian S. Addy, Ahmed Askora, Takeru Kawasaki, Makoto Fujie, and Takashi Yamada
    • Journal Title

      PHYTOPATHOLOGY

      Volume: Vol. 102 Issue: 5 Pages: 469-477

    • DOI

      10.1094/phyto-11-11-0319-r

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Filamentous PhageφRSS1 Enhances Virulence of Phytopathogenic Ralstonia solanacearum on Tomato2012

    • Author(s)
      Hardian S. Addy, Ahmed Askora, Takeru Kawasaki, Makoto Fujie, and Takashi Yamada
    • Journal Title

      PHYTOPATHOLOGY

      Volume: Vol. 102 Issue: 3 Pages: 241-245

    • DOI

      10.1094/phyto-10-11-0277

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 青枯病菌の繊維状ファージ:病原性の諸刃の剣2014

    • Author(s)
      山田 隆
    • Organizer
      日本植物病理学会 植物細菌病談話会
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Molecular characterization of a new filamentous phage infecting Ralstonia solanacearum with a wide host range2014

    • Author(s)
      Askora, A., Kawasaki, T., Fujie, M., Yamada, T.
    • Organizer
      第66回日本生物工学会大会
    • Related Report
      2014 Annual Research Report
  • [Presentation] Isolation and genomic charactertization of new T7-like phages infecting Ralstonia solanacearum from Thailand2014

    • Author(s)
      Erlia Narulita, Sonomoto, T., Kawasaki, T., Fujie, M., Yamada, T.
    • Organizer
      第66回日本生物工学会大会
    • Related Report
      2014 Annual Research Report
  • [Presentation] 青枯病菌とファージのダイナミックな相互作用の解析2014

    • Author(s)
      園元貴也、川崎 健、藤江 誠、山田 隆、緒方博之
    • Organizer
      第66回日本生物工学会大会
    • Related Report
      2014 Annual Research Report
  • [Presentation] 青枯病菌に感染するT7型ファージゲノムのダイナミックな再編成2012

    • Author(s)
      小寺 星、他4名
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2012-10-24
    • Related Report
      2012 Annual Research Report
  • [Presentation] 青枯病菌に感染する3種のT7型ファージの系統解析と感染実験2012

    • Author(s)
      松波美奈穂、川崎健,藤江誠、山田隆
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2012-10-24
    • Related Report
      2012 Annual Research Report
  • [Presentation] バクテリオファージ耐性青枯病菌における変異遺伝子解析2012

    • Author(s)
      藤澤真理子, 他4名
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2012-10-24
    • Related Report
      2012 Annual Research Report
  • [Presentation] RSL型ジャンボファージの構造解析と持続的宿主抑制機構.2012

    • Author(s)
      濱崎良介, 他4名
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2012-10-24
    • Related Report
      2012 Annual Research Report
  • [Presentation] RSS型繊維状ファージはXerCD/dif機構によって宿主ゲノムに組み込まれる2012

    • Author(s)
      田坂友一、川崎健,藤江誠、山田隆
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2012-10-24
    • Related Report
      2012 Annual Research Report
  • [Presentation] 青枯病菌に感染するT7型ファージゲノムのダイナミックな再編成

    • Author(s)
      小寺 星、藤原亜希子、川崎 健、藤江 誠、山田 隆
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島国際会議場(広島市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 青枯病菌に感染する3種のT7型ファージの系統解析と感染実験

    • Author(s)
      松波美奈穂、川崎 健、藤江 誠、山田 隆
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島国際会議場(広島市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 青枯病菌ジャンボファージRSL1の構造と機能に関する研究

    • Author(s)
      竹内秀樹、川崎 健、藤江 誠、山田 隆
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島国際会議場(広島市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 青枯病菌繊維状ファージRSMの宿主制御機構に関する研究

    • Author(s)
      薮谷祐司、川崎 健、藤江 誠、山田 隆
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島国際会議場(広島市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] History and pogress of phage biocontrpl in agriculrure

    • Author(s)
      YAMADA Takashi
    • Organizer
      第65回日本生物工学会大会, Int Natl Symp “Challenge of Red Queen”
    • Place of Presentation
      広島国際会議場(広島市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ファージを利用した青枯病防除技術の開発

    • Author(s)
      山田 隆
    • Organizer
      平成25年度野菜茶業試験研究推進会議 野菜病害虫部会野菜病害虫研究会
    • Place of Presentation
      (独)農業・食品産業技術総合研究機構 野菜茶業研究所
    • Related Report
      2013 Annual Research Report
  • [Book] Bacteriophages of Ralstonia solanacearum : their diversity and utilization as biocontrol agents in agriculture. In : Bacteriophage (I. Kurtboke, Ed.) ISBN 978-953-51-0272-42012

    • Author(s)
      Yamada, T.
    • Publisher
      InTech-Open Access Publisher,
    • Related Report
      2012 Annual Research Report
  • [Remarks] 広島大学大学院先端物質科学研究科生命分子情報学研究室ホームページ

    • URL

      http://home.hiroshima-u.ac.jp/mbiotech/ichikou/index.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 広島大学大学院先端物質科学研究科分子生命機能科学専攻生命分子情報学研究室ホームページ

    • URL

      http://home.hiroshima-u.ac.jp/mbiotech/ichikou/index.html

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-12-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi