Sustainable control of bacterial wilt by jumbo phage technology
| Project/Area Number |
24380049
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Hiroshima University |
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Principal Investigator |
YAMADA Takashi 広島大学, 先端物質科学研究科, 教授 (40230461)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIE Makoto 広島大学, 大学院先端物質科学研究科, 准教授 (20274110)
KAWASAKI Takeru 広島大学, 大学院先端物質科学研究科, 助教 (00510299)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2012: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | bacteriophage / bacterial wilt / Ralstonia solanacearum / biocontrol / resistance cost / prophage induction / growth inhibition / loss of virulence / 青枯病 / バクテリオファージ / ジャンボファージ / バイオコントロール |
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| Outline of Final Research Achievements |
Four major mechanisms have been found to effects host virulence after infection by RSL1: (1) twitching motility and associated virulence were affected in the host cells,(2) among 12 RSL1 genes enhanced during prolonged infection, orf105, orf106, and orf121 worked for growth activation but orf137 and orf209 strongly inhibited the growth, (3) RSL1 induced RSS prophages in the host cells, and (4) phage resistance working in the host was associated with LPS structure and function, so that resistant cells all lost virulence because of LPS deficiency.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] The filamentous phage XacF1 causes loss of virulence in Xanthomonas axonopodis pv. citri, the causative agent of citrus canker disease.2014
Author(s)
Ahmad, A.A., Askora, A., Kawasaki,T., Fujie, M., and Yamada,T.
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Journal Title
Front. Microbiol.
Volume: 5
Pages: 3211-11
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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