Development of antipathogenic agents targeting quorum sensing of Gram-positive bacteria
| Project/Area Number |
24380050
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
NAKAYAMA Jiro 九州大学, (連合)農学研究科(研究院), 准教授 (40217930)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NAGATA Koji 東京大学, 大学院農学生命科学研究科, 准教授 (30280788)
IGARASHI Yasuhiro 富山県立大学, 工学部, 教授 (20285159)
SUZUKI Takashi 愛媛大学, 医学部附属病院, 講師 (70398048)
SHIMIZU Tohru 金沢大学, 医学系, 教授 (80235655)
OHTANI Kaori 金沢大学, 医学系, 講師 (30377410)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
|
|---|
| Keywords | クオラムセンシング / グラム陽性細菌 / 日和見感染 / 抗感染症剤 / ペプチドデザイン / 阻害剤開発 / クォルモン / アンタゴニスト / グラム陽性日和見感染菌 / 阻害剤スクリーニング / デプシペプチド / ゼラチナーゼ / 眼内炎 |
|---|
| Outline of Final Research Achievements |
Quorum sensing mediated by cyclic peptide as an autoinducer (AIP) commonly controls the expression of virulence in pathogenic Gram-positive bacteria such as staphylococci, enterococci and clostridia. In this study, we performed screening and drug design of quorum sensing inhibitors (QSI). Consequently, we found that a number of cyclic peptides of microbial secondary metabolites, e.g., WS9326A and Avellanin C, showed antagonistic activity against AIP at micromolar concentrations and attenuated virulences of Gram-positive pathogens. Further, we succeeded in designing of QSI peptides based on the structure of AIP of Clostridium perfringens, which attenuated the expression of theta toxin gene at submicromolar concentrations
|
Report
(4 results)
Research Products
(22 results)
-
[Journal Article] Avellanin C, an inhibitor of quorum sensing signaling in Staphylococcus aureus, from Hamigera ingelheimensis2015
Author(s)
Yasuhiro Igarashi, Fumiya Gohda, Taito Kadoshima, Takao Fukuda, Tomoaki Hanafusa, Akane Shojima, Jiro Nakayama, Gerald F. Bills, Steven Williams
-
Journal Title
Journal of Antibiotics
Volume: in press
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
[Journal Article] Development of a peptide antagonist against fsr quorum sensing of Enterococcus faecalis.2013
Author(s)
Jiro Nakayama, Ryoji Yokohata, Mami Sato, Takashi Suzuki, Takahisa Matsufuji, Kenzo Nishiguchi, Takeshi Kawai, Yosuke Yamanaka, Koji Nagata, Masaru Tanokura, Kenji Sonomoto
-
Journal Title
ACS Chem. Biol.
Volume: 8
Issue: 4
Pages: 804-811
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Development of a peptide antagonist against fsr quorum sensing of Enterococcus faecalis2013
Author(s)
Nakayama J, Yokohata R, Sato M, Suzuki T, Matsufuji T, Nishiguchi K, Kawai T, Yamanaka Y, Nagata K, Tanokura M, Sonomolo K
-
Journal Title
ACS chemical biology
Volume: (in press)
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
