| Project/Area Number |
24380050
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Kyushu University |
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Principal Investigator |
NAKAYAMA Jiro 九州大学, (連合)農学研究科(研究院), 准教授 (40217930)
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| Co-Investigator(Kenkyū-buntansha) |
NAGATA Koji 東京大学, 大学院農学生命科学研究科, 准教授 (30280788)
IGARASHI Yasuhiro 富山県立大学, 工学部, 教授 (20285159)
SUZUKI Takashi 愛媛大学, 医学部附属病院, 講師 (70398048)
SHIMIZU Tohru 金沢大学, 医学系, 教授 (80235655)
OHTANI Kaori 金沢大学, 医学系, 講師 (30377410)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Keywords | クオラムセンシング / グラム陽性細菌 / 日和見感染 / 抗感染症剤 / ペプチドデザイン / 阻害剤開発 / クォルモン / アンタゴニスト / グラム陽性日和見感染菌 / 阻害剤スクリーニング / デプシペプチド / ゼラチナーゼ / 眼内炎 |
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| Outline of Final Research Achievements |
Quorum sensing mediated by cyclic peptide as an autoinducer (AIP) commonly controls the expression of virulence in pathogenic Gram-positive bacteria such as staphylococci, enterococci and clostridia. In this study, we performed screening and drug design of quorum sensing inhibitors (QSI). Consequently, we found that a number of cyclic peptides of microbial secondary metabolites, e.g., WS9326A and Avellanin C, showed antagonistic activity against AIP at micromolar concentrations and attenuated virulences of Gram-positive pathogens. Further, we succeeded in designing of QSI peptides based on the structure of AIP of Clostridium perfringens, which attenuated the expression of theta toxin gene at submicromolar concentrations
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