Structural analysis of novel bifidobacterial enzymes degrading human and plant glycans
Project/Area Number |
24380053
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Applied biochemistry
|
Research Institution | The University of Tokyo |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
ASHIDA Hisashi 近畿大学, 生物理工学部, 教授 (40370987)
FUJITA Kiyotaka 鹿児島大学, 農学部, 准教授 (20381189)
芦田 久 近畿大学, 生物理工学部, 教授 (40379087)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
|
Keywords | 糖質分解酵素 / ビフィズス菌 / 血液型抗原 / β-アラビノオリゴ糖 / 複合糖鎖 |
Outline of Final Research Achievements |
We determined the crystal structure of GH127 beta-L-arabinofuranosidase (HypBA1). HypBA1 has a Cys residue at its active center and was the first "cysteine glycosidase". We found four new glycosidases from B. bifidum. Moreover, we revealed the transcription regulation mechanism of the beta-arabinooligosaccharide degradation system in B. longum and investigated characteristics of a HypBA1 homolog.
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Report
(4 results)
Research Products
(52 results)