Identification of diabetogenic and obesity genes by using nucleotides sequence variations between SM/J and A/J mice.
| Project/Area Number |
24380068
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Nagoya University |
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Principal Investigator |
HORIO Fumihiko 名古屋大学, 生命農学研究科, 教授 (20165591)
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| Co-Investigator(Renkei-kenkyūsha) |
OHNO Tamio 名古屋大学, 大学院医学系研究科, 准教授 (90293620)
KOBAYASHI Misato 名古屋大学, 大学院生命農学研究科, 助教 (20456586)
OHNO Kinji 名古屋大学, 大学院医学系研究科, 教授 (80397455)
IHARA Kunio 名古屋大学, 遺伝子実験施設, 准教授 (90223297)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Keywords | 糖尿病遺伝子 / 肥満遺伝子 / マウス / 高脂肪食 / 脂肪肝遺伝子 |
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| Outline of Final Research Achievements |
We had mapped 10 quantitative traits loci (QTLs) for diabetes, obesity and fatty liver on chromosomes of SM/J and A/J mice under feeding a high-fat diet. In this study, we developed exome analyses of SM/J and A/J mice geneomes by using next generation sequencer, and found out a lot of nucleotides mutations between these two strains exons. By using this mutation list, we extracted Iah1 as a candidate gene for fatty liver on chromosome 12. The overexpression of Iah1 gene in Hepa-1 cell (mouse hepatoma cell) caused the reduction of intracellular triglycerides, and changed the expressions of lipogenesis-related genes. We are establishing the Iah1-knockout mouse for clarifying the incidence of fatty liver by depleting the Iah1 expression, and then Iah1 will be identified as a fatty liver gene.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Searching for genomic region of high-fat diet-induced type 2 diabetes in mouse chromosome 2 by analysis of congenic strains2014
Author(s)
4.Kobayashi M, Ohno T, Ihara K, Murai, A., Kumazawa, M., Hoshino, H., Iwanaga, K., Iwai, H., Hamana, Y., Ito, M., Ohno, K., Horio, M..
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Journal Title
Plos One
Volume: 9
Issue: 5
Pages: e96271-e96271
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Ternatin, a cyclic peptide isolated from mushroom, and its derivative suppress hyperglycemia and hepatic fatty acid synthesis in spontaneously diabetic KK-Ay mice2012
Author(s)
M. Kobayashi, H. Kawashima, K. Takemori, H. Ito, A. Murai, S. Masuda, K. Yamada, D. Uemura, F. Horio
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 427
Issue: 2
Pages: 299-304
DOI
Related Report
Peer Reviewed
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