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Identification of diabetogenic and obesity genes by using nucleotides sequence variations between SM/J and A/J mice.

Research Project

Project/Area Number 24380068
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Food science
Research InstitutionNagoya University

Principal Investigator

HORIO Fumihiko  名古屋大学, 生命農学研究科, 教授 (20165591)

Co-Investigator(Renkei-kenkyūsha) OHNO Tamio  名古屋大学, 大学院医学系研究科, 准教授 (90293620)
KOBAYASHI Misato  名古屋大学, 大学院生命農学研究科, 助教 (20456586)
OHNO Kinji  名古屋大学, 大学院医学系研究科, 教授 (80397455)
IHARA Kunio  名古屋大学, 遺伝子実験施設, 准教授 (90223297)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Keywords糖尿病遺伝子 / 肥満遺伝子 / マウス / 高脂肪食 / 脂肪肝遺伝子
Outline of Final Research Achievements

We had mapped 10 quantitative traits loci (QTLs) for diabetes, obesity and fatty liver on chromosomes of SM/J and A/J mice under feeding a high-fat diet. In this study, we developed exome analyses of SM/J and A/J mice geneomes by using next generation sequencer, and found out a lot of nucleotides mutations between these two strains exons. By using this mutation list, we extracted Iah1 as a candidate gene for fatty liver on chromosome 12. The overexpression of Iah1 gene in Hepa-1 cell (mouse hepatoma cell) caused the reduction of intracellular triglycerides, and changed the expressions of lipogenesis-related genes. We are establishing the Iah1-knockout mouse for clarifying the incidence of fatty liver by depleting the Iah1 expression, and then Iah1 will be identified as a fatty liver gene.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (10 results)

All 2015 2014 2013 2012 Other

All Journal Article (5 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (5 results)

  • [Journal Article] Searching for genomic region of high-fat diet-induced type 2 diabetes in mouse chromosome 2 by analysis of congenic strains2014

    • Author(s)
      4.Kobayashi M, Ohno T, Ihara K, Murai, A., Kumazawa, M., Hoshino, H., Iwanaga, K., Iwai, H., Hamana, Y., Ito, M., Ohno, K., Horio, M..
    • Journal Title

      Plos One

      Volume: 9 Issue: 5 Pages: e96271-e96271

    • DOI

      10.1371/journal.pone.0096271

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Searching for genomic region of high-fat diet-induced type 2 diabetes in mouse chromosome 2 by analysis of congenic strains.2014

    • Author(s)
      Kobayashi,M., Horio,F. 他10名
    • Journal Title

      PLOS ONE

      Volume: in press

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ternatin, a cyclic peptide isolated from mushroom, and its derivative suppress hyperglycemia and hepatic fatty acid synthesis in spontaneously diabetic KK-Ay mice2012

    • Author(s)
      M. Kobayashi, H. Kawashima, K. Takemori, H. Ito, A. Murai, S. Masuda, K. Yamada, D. Uemura, F. Horio
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 427 Issue: 2 Pages: 299-304

    • DOI

      10.1016/j.bbrc.2012.09.045

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Coffee improves insulin-stimulated Akt phosphorylation in liver and skeletal muscle in diabetic KK-Ay mice.2012

    • Author(s)
      Kobayashi, M., Horio, F., 他8名
    • Journal Title

      J.Nutr.Sci.Vitaminol.,58,408-414(2012)

      Volume: 58 Pages: 408-414

    • NAID

      130003368136

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 2型糖尿病モデルを用いた糖尿病発症因子解析の最新動向2012

    • Author(s)
      小林美里、堀尾文彦
    • Journal Title

      日本臨床

      Volume: 70 Pages: 202-206

    • Related Report
      2012 Annual Research Report
  • [Presentation] コンジェニックマウスを用いた食餌誘導性脂肪肝感受性遺伝子の存在領域の解析2015

    • Author(s)
      鈴木京、小林美里、大野民生、辻村絢子、村井篤嗣、堀尾文彦
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山
    • Year and Date
      2015-03-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 脂肪肝感受性遺伝子座(Fl1sa)の候補遺伝子の発現制御機構の解析とコンジェニックマウスによる責任遺伝子の探索2014

    • Author(s)
      鈴木 京、小林美里、大野民生、辻村絢子、小田裕昭、村井篤嗣、堀尾文彦
    • Organizer
      第68回日本栄養・食糧学会大会
    • Place of Presentation
      江別
    • Year and Date
      2014-05-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] 脂肪肝感受性を規定する候補遺伝子の肝細胞脂質代謝に対する作用の解析2013

    • Author(s)
      都築佳奈、小林美里、鈴木 京、大野民生、村井篤嗣、堀尾文彦
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      仙台
    • Year and Date
      2013-03-26
    • Related Report
      2012 Annual Research Report
  • [Presentation] 高脂肪食誘発型2型糖尿病の原因遺伝子の解析 : 新規モデルマウスを用いて2012

    • Author(s)
      堀尾文彦
    • Organizer
      第66回日本栄養・食糧学会大会(仙台)
    • Place of Presentation
      仙台(招待講演)
    • Year and Date
      2012-05-20
    • Related Report
      2012 Annual Research Report
  • [Presentation] マウス第12番染色体の脂肪肝感受性遺伝子座に存在する遺伝子のエクソーム解析

    • Author(s)
      小林美里、立石壮志、鈴木 京、大野民生、大野欽司、伊藤美佳子、村井篤嗣、堀尾文彦
    • Organizer
      第67回日本栄養・食糧学会大会
    • Place of Presentation
      名古屋
    • Related Report
      2013 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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