Studies on the mechanism of manifestation of fatigue and its effects on the attenuation of motivation to move and on the possibility of their control by ingestion of foods/food components

• Project/Area Number: 24380070
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Kyoto University
• Principal Investigator: INOUE Kazuo 京都大学, (連合)農学研究科(研究院), 准教授 (80213148)
• Co-Investigator(Kenkyū-buntansha): TSUZUKI Satoshi 京都大学, 大学院農学研究科, 助教 (50283651)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000); Fiscal Year 2012: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
• Keywords: 疲労 / 脳内自己刺激 / 脳報酬系 / ストレス

Outline of Final Research Achievements

(1) Intra-cranial self-stimulation could be used for determination of fatigue (or attenuation of the motivation to move) induced by both physical and psychological load in rat. (2) By using this system force-feeding of tartrate suggested to attenuate manifestation of fatigue/decrease in the motivation to move caused by treadmill running. Time course changes in extracellular concentration of dopamine (DA) in nucleus accumbens shell and core during treadmill running in rat were determined. (3) The increase in blood lactate induced by physical exercise was detected by both the brain and the liver, and this lead the activation of TGF-β in cerebrospinal fluid. Increase in the concentration of active form of TGF-β has already been shown to cause fatigue-like behavior in animals. (4) Force-feeding of catechin was suggested to increase endurance capacity in mice by using adjustable-current swimming pool system.

Research Products

• [Journal Article] Central fatigue and TGF-β (2013)
  • Author(s): Kazuo Inoue
  • Journal Title: Advances in Neuroimmune Biology Volume: 4 Issue: 4 Pages: 229-236
  • DOI: 10.3233/nib-130069
• [Presentation] 持久運動時の脳報酬系機能に関する研究 (2015)
  • Author(s): 石井仁、横山小夜香、井上和生、伏木亨
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山市、岡山大学
  • Year and Date: 2015-03-27
• [Presentation] 脳内自己刺激を用いた中枢性疲労の新規測定法確立とその応用 (2015)
  • Author(s): 井樋聡美、井上和生、伏木亨
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山市、岡山大学
  • Year and Date: 2015-03-27
• [Presentation] 持久運動時のNAc shellにおける神経活動の解明 (2014)
  • Author(s): 横山小夜香、井上和生、伏木亨
  • Organizer: 第68回日本栄養・食糧学会大会
  • Place of Presentation: 江別市、酪農学園大学
  • Year and Date: 2014-05-31
• [Presentation] 3Intracisternal self stimulation (ICSS) を用いた新規中枢性疲労測定法の確立 (2014)
  • Author(s): 井樋聡美、上野聡子、永吉宏充、井上和生、伏木亨
  • Organizer: 日本農芸化学会
  • Place of Presentation: 川崎市 (明治大学生田キャンパス)
• [Presentation] 持久運動時のNAc shellにおける神経活動の解明 (2013)
  • Author(s): 横山小夜香、井上和生、伏木亨
  • Organizer: マイクロダイアリシス研究会
  • Place of Presentation: 東京 (お茶の水女子大学)