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Development of novel assay of beta-glycanases for complete enzymatic saccharification of unused biomass

Research Project

Project/Area Number 24380089
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Wood science
Research InstitutionThe University of Tokyo

Principal Investigator

IGARASHI Kiyohiko  東京大学, 農学生命科学研究科, 准教授 (80345181)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2012: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywordsバイオマス / βーグリカナーゼ / セルラーゼ / キチナーゼ / 結晶性多糖 / セルロース / キチン / プロセッシビティ / 糖化 / グリカナーゼ
Outline of Final Research Achievements

Cellulose and chitin are the main components of unused biomass, and effective utilization of these materials is expected as source of carbon of the next generation without competition with food. Considering the polysaccharides share the common feature consisting with beta-linked glycosides, it is the key to utilize the unused biomass how to control degrading enzymes efficiently at a solid/liquid interface.
In the present research, the behavior of beta-glycanase such as cellulase from filamentous fungi and cellulase and chitinase from bacteria was observed qualitatively and quantitatively at a solid/liquid interface by high-speed atomic force microscopy, and a strategy for the effective hydrolysis of unused biomass using a biochemical process was built.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (23 results)

All 2015 2014 2013 2012 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Invited: 5 results) Book (1 results) Remarks (6 results)

  • [Journal Article] Trade-off between Processivity and Hydrolytic Velocity of Cellobiohydrolases at the Surface of Crystalline Cellulose2014

    • Author(s)
      Akihiko Nakamura, Watanabe, H., Ishida, T., Uchihashi, T., Wada, M., Ando, T., Igarashi, K. and Samejima, M.
    • Journal Title

      Journal of the American Chemical Society

      Volume: 136 Issue: 12 Pages: 4584-4592

    • DOI

      10.1021/ja4119994

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single-molecule Imaging Analysis of Elementary Reaction Steps of Trichoderma reesei Cellobiohydrolase I (Cel7A) Hydrolyzing Crystalline Cellulose Iα and IIII2014

    • Author(s)
      Y.Shibafuji, A.Nakamura, T.Uchihashi, N.Sugimoto, S.Fukuda, H.Watanabe, M.Samejima, T.Ando, H.Noji, A.Koivula, K.Igarashi, R.Iino
    • Journal Title

      Journal of Biological Chemistry

      Volume: 289 (29) Issue: 20 Pages: 1405614065-1405614065

    • DOI

      10.1074/jbc.m113.546085

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Two-way traffic of glycoside hydrolase family 18 processive chitinases on crystalline chitin2014

    • Author(s)
      Igarashi, K., Uchihashi, T., Uchiyama, T., Sugimoto, H., Wada, M., Suzuki, K., Sakuda, S., Ando, T., Watanabe, T., and Samejima, M.
    • Journal Title

      Nature Commun.

      Volume: 5 Issue: 1 Pages: 3975-3975

    • DOI

      10.1038/ncomms4975

    • URL

      https://pure.teikyo.jp/en/publications/6f0f27b4-a91e-4028-8f9e-00dc47539bda

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] High-speed atomic force microscope combined with single-molecule fluorescence microscope.2013

    • Author(s)
      S. Fukuda, T. Uchihashi, R. Iino, Y. Okazaki, M. Yoshida, K. Igarashi, T. Ando
    • Journal Title

      Rev. Sci. Instrum.

      Volume: 84 Issue: 7 Pages: 73706-73706

    • DOI

      10.1063/1.4813280

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Tryptophan Residue at the Active Site Tunnel Entrance of Trichoderma reesei Cellobiohydrolase Ce17A Is Important for Initiation of Degradation of Crystalline Cellulose2013

    • Author(s)
      Akihiko Nakamura, Tsukada, T., Auer, S., Furuta, T., Wada, M., Koivula, A., Igarashi, K. and Samejima M.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 288 Issue: 19 Pages: 13503-13510

    • DOI

      10.1074/jbc.m113.452623

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Degradation of Crystalline Celluloses by <I>Phanerochaete chrysosporium </I>Cellobiohydrolase II (Cel6A) Heterologously Expressed in Methylotrophic Yeast <I>Pichia pastoris</I>2012

    • Author(s)
      Igarashi, K., Maruyama, M., Nakamura, A., Ishida, T., Wada, M., and Samejima, M.
    • Journal Title

      Journal of Applied Glycoscience

      Volume: 59 Issue: 3 Pages: 105-110

    • DOI

      10.5458/jag.jag.JAG-2011_029

    • NAID

      130004481005

    • ISSN
      1344-7882, 1880-7291
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adsorption characteristics of fungal family 1 cellulose-binding domain from Trichoderma reesei cellobiohydrolase I on crystalline cellulose: Negative cooperative adsorption via a steric exclusion effect2012

    • Author(s)
      Sugimoto, N., Igarashi, K., Wada, M., and Samejima, M.
    • Journal Title

      Langmuir

      Volume: 28 Issue: 40 Pages: 14323-14329

    • DOI

      10.1021/la302352k

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visualization of cellobiohydrolase I from Trichoderma reesei moving on crystalline cellulose using high-speed atomic force microscopy2012

    • Author(s)
      Kiyohiko Igarashi
    • Journal Title

      Methods in Enzymology

      Volume: 510 Pages: 169-182

    • DOI

      10.1016/b978-0-12-415931-0.00009-4

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] セルロース資化性放線菌Cellulomonas fimi由来のセルラーゼの性状解析2015

    • Author(s)
      内山拓、中村彰彦、五十嵐圭日子、鮫島正浩、金子哲、内橋貴之
    • Organizer
      2015年度農芸化学会大会
    • Place of Presentation
      岡山大学津島キャンパス 岡山
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Processivity in enzymatic degradation of crystalline polysaccharides: cellulases and chitinases2014

    • Author(s)
      Kiyohiko Igarashi
    • Organizer
      Mie Bioforum 2014
    • Place of Presentation
      Nemuno Sato Hotel & Resort 三重
    • Year and Date
      2014-11-18 – 2014-11-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] セルロース系バイオマスを分解する酵素の構造機能相関2014

    • Author(s)
      五十嵐圭日子
    • Organizer
      学術振興会第169委員会 第45回研究会
    • Place of Presentation
      ゆうぽうと 東京
    • Year and Date
      2014-07-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 糖質加水分解酵素ファミリー6セルラーゼのプロセッシビティを中心とした機能解析2014

    • Author(s)
      内山拓、中村彰彦、五十嵐圭日子、鮫島正浩、金子哲、内橋貴之
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] Cellulases: cooperative biomass breakdown2014

    • Author(s)
      Kiyohiko Igarashi
    • Organizer
      JST-ALCA international workshop for plant cell wall engineering
    • Place of Presentation
      Tokyo
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 結晶性セルロースとキチンを分解する酵素 : プロセッシブな加水分解の分子機構とドメイン構造における戦略2013

    • Author(s)
      五十嵐圭日子
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学(招待講演)
    • Year and Date
      2013-03-27
    • Related Report
      2012 Annual Research Report
  • [Presentation] 微生物におけるセルロースとキチンの酵素分解戦略:結晶性構造多糖を利用するためのヒント2013

    • Author(s)
      五十嵐圭日子
    • Organizer
      第7回多糖の未来フォーラム
    • Place of Presentation
      大阪
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] プロセッシブグリコシダーゼはどうやって結晶性多糖の上を走るのか?2013

    • Author(s)
      五十嵐圭日子
    • Organizer
      第3回分子モーター討論会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Book] 植物細胞壁2013

    • Author(s)
      五十嵐圭日子
    • Total Pages
      349
    • Publisher
      講談社
    • Related Report
      2012 Annual Research Report
  • [Remarks] やはりカメはウサギに勝る

    • URL

      http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/the-tortoise-beats-the-hare-again/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 分解酵素にとって結晶性キチンは双方向の高速道路だった

    • URL

      http://www.a.u-tokyo.ac.jp/topics/2014/20140605-1.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 高速で逆送する酵素がキチンを分解

    • URL

      http://scienceportal.jst.go.jp/news/newsflash_review/newsflash/2014/06/20140609_03.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 糸状菌由来セルラーゼがセルロース分子鎖の末端を認識する機構の解明

    • URL

      http://www.a.u-tokyo.ac.jp/topics/2013/20130510-2.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 結晶性セルロースの分解は、切断の「速さ」ではなく基質を掴み続ける「ねばり強さ」が重要である

    • URL

      http://www.a.u-tokyo.ac.jp/topics/2014/20140313-4.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] やはりカメはウサギに勝る

    • URL

      http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/the-tortoise-beats-the-hare-again/

    • Related Report
      2013 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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