| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Outline of Final Research Achievements |
I conducted comprehensive cDNA cloning of SLRPs in zebrafish and goldfish, and 10 new sequences were deposited to the database (DDBJ). Then, spatiotemporal expressions of mRNAs of decorin (dcn), biglycan (bgn), chondroadherin (chad), lumican (lum), osteoglycin (ogn) and dermatopontin (dpt) were clarified. In addition, recombinant proteins of Dcn, Bgn and Dpt and their specific antibodies were produced, and immunohistochemical localizations of these proteins were clarified. Hybrid gel made of recombinant Dpt and type I collagen was developed. Moreover, dpt-knock down experiment revealed that Dpt regulated m RNA expressions, fibril diameter and alignment of both type I and type II collagens in zebrafish. All these data are new to science, and the present results opened the possibility of new hybrid scaffolds consisted of collagen fibrils and SLRPs/Dpt.
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