Studies on the function of non-collagenous proteins contained in the goldfish scales on the regulation of collagen fibril diameter and alignment.
| Project/Area Number |
24380101
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Hokkaido University |
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Principal Investigator |
Takagi Yasuaki 北海道大学, 水産科学研究科(研究院), 教授 (10212002)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Keywords | 魚類コラーゲン / 非コラーゲン性基質タンパク質 / SLRPs / デルマトポンチン / 組織工学 / 生物材料・バイオマテリアル / 細胞足場材料 / コラーゲン線維配向 / 鱗 / 生体修復材料 / 再生医療 / チョウザメ / 配向 / dermatopontin / コラーゲン配向 / 生態修復材料 / ゼブラフィッシュ / ノックダウン / 細胞外基質タンパク質 |
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| Outline of Final Research Achievements |
I conducted comprehensive cDNA cloning of SLRPs in zebrafish and goldfish, and 10 new sequences were deposited to the database (DDBJ). Then, spatiotemporal expressions of mRNAs of decorin (dcn), biglycan (bgn), chondroadherin (chad), lumican (lum), osteoglycin (ogn) and dermatopontin (dpt) were clarified. In addition, recombinant proteins of Dcn, Bgn and Dpt and their specific antibodies were produced, and immunohistochemical localizations of these proteins were clarified. Hybrid gel made of recombinant Dpt and type I collagen was developed. Moreover, dpt-knock down experiment revealed that Dpt regulated m RNA expressions, fibril diameter and alignment of both type I and type II collagens in zebrafish. All these data are new to science, and the present results opened the possibility of new hybrid scaffolds consisted of collagen fibrils and SLRPs/Dpt.
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Report
(5 results)
Research Products
(25 results)
