Behavioral and molecular biology of sensing mechanism for tetrodotoxin in Takifugu rubiripes

• Project/Area Number: 24380109
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: General fisheries
• Research Institution: Nagasaki University
• Principal Investigator: SAKAKURA Yoshitaka 長崎大学, 水産・環境科学総合研究科(水産), 教授 (20325682)
• Co-Investigator(Renkei-kenkyūsha): KINOSHITA Shigeharu 東京大学, 農学生命科学研究科, 准教授 (40401179) ; TAKATANI Tomohiro 長崎大学, 水産・環境科学総合研究科(水産), 教授 (90304972)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: 水産学 / 行動学 / 生理活性 / 脳・神経 / 遺伝子 / マイクロアレイ

Outline of Final Research Achievements

In order to investigate ecological meanings of tetrodotoxin (TTX) for pufferfish, sensing, ingestion and mode of action in the brain of TTX were investigated using non-toxic hatchery-reared Takifugu rubripes. Behavioral analysis by Y-maze test revealed that a pufferfish senses TTX by olfaction and ingests orally, and TTX was immediately distributed into liver, skin and central nervous system (brain, eye and olfactory organ). Gene expression analysis in the brain of pufferfish which sensed TTX was conducted using a next generation sequencer. As a result, genes involving odorant receptors, feeding-stimulating peptides and reward-system upregulated, which supports the findings of behavioral and physiological observations.

Research Products

• [Journal Article] トラフグ人工種苗の成長に伴う行動と食性の変化 (2015)
  • Author(s): 山根 晃・吉田 歩・山崎英樹・崎山一孝・河端雄毅・阪倉良孝
  • Journal Title: 水産増殖 Volume: ６３
  • NAID: 130005157558
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 書評「毒魚の自然史－毒の謎を追う」 (2015)
  • Author(s): 阪倉良孝
  • Journal Title: 魚類学雑誌 Volume: 62 Pages: 74-75
• [Journal Article] Larval pufferfish protected by maternal tetrodotoxin (2014)
  • Author(s): Itoi, S., Yoshikawa, S., Asahina, K., Suzuki, M., Ishizuka, K., Takimoto, N., Mitsuoka, R., Yokoyama, N., Detake, A., Takayanagi, C., Eguchi, M., Tatsuno, R., Kawane, M., Kokubo, S., Takanashi, S., Miura, A., Suitoh, K., Takatani, T., Arakawa, O., Sakakura, Y., Sugita, H.
  • Journal Title: Toxicon Volume: 78 Pages: 35-40
  • DOI: 10.1016/j.toxicon.2013.11.003
  • NAID: 120006986794
  • Peer Reviewed / Open Access
• [Journal Article] Puffer smells tetrodotoxin (2013)
  • Author(s): Okita, K., Yamazaki, H., Sakiyama, K., Yamane, H., Niina, S., Takatani, T., Arakawa, O., Sakakura, Y.
  • Journal Title: Ichthyological Research Volume: 60 Issue: 4 Pages: 386-389
  • DOI: 10.1007/s10228-013-0353-z
  • NAID: 120006986034
  • Peer Reviewed
• [Journal Article] Comparison of the localization of tetrodotoxin between wild pufferfish Takifugu rubripes juveniles and hatchery-reared juveniles with tetrodotoxin administration (2013)
  • Author(s): K. Okita, T. Takatani, J. Nakayasu, H. Yamazaki, K. Sakiyama, K. Ikeda, O. Arakawa, Y. Sakakura
  • Journal Title: Toxicon Volume: 71 Pages: 128-133
  • DOI: 10.1016/j.toxicon.2013.05.018
  • NAID: 120006986203
  • Peer Reviewed
• [Presentation] Evaluation of Fish Quality for Release in the Hatchery-Reared Tiger Puffer Juveniles (2014)
  • Author(s): Sakakura, Y., Yamane, H. Yamazaki, H., Sakiyama, K.
  • Organizer: 11th Korea-Japan, Japan-Korea Joint Symposium for Aquaculture
  • Place of Presentation: 群山大学校（韓国・群山市）
  • Year and Date: 2014-10-31 – 2014-11-01
• [Presentation] フグ毒を投与したトラフグ人工種苗の嗅上皮および脳のトランスクリプトーム解析 (2014)
  • Author(s): 沖田光玄・佐藤根妃奈・陳盈光・木下滋晴・浅川修一・山崎英樹・崎山一孝・高谷智裕・荒川修・阪倉良孝
  • Organizer: 平成26年度日本水産学会秋季大会
  • Place of Presentation: 九州大学（福岡県福岡市）
  • Year and Date: 2014-09-19 – 2014-09-22
• [Presentation] トラフグ稚魚のフグ毒感知、摂取、および脳内蓄積に関連する遺伝子の発現 (2014)
  • Author(s): 沖田光玄・平野 雪・木下滋晴・小島大輔・山崎英樹・崎山一孝・高谷智裕・荒川 修・阪倉良孝
  • Organizer: 平成26年度日本水産学会春季大会
  • Place of Presentation: 北海道大学函館キャンパス（北海道函館市）
• [Presentation] フグの仔魚は母親由来のTTXによって守られている (2014)
  • Author(s): 糸井史朗・吉川沙織・朝比奈 潔・鈴木美和・石塚健人・瀧本成美・光岡涼子・横山直人・出竹歩美・高柳智江・江口美帆・小久保翔太・高梨志保里・三浦 愛（・河根三雄・水藤勝喜・辰野竜平・高谷智裕・荒川 修・阪倉良孝・杉田治男
  • Organizer: 平成26年度日本水産学会春季大会
  • Place of Presentation: 北海道大学函館キャンパス（北海道函館市）
• [Presentation] トラフグのフグ毒感知に関わる遺伝子のマイクロアレイ解析による探索 (2013)
  • Author(s): 沖田光玄・後藤完奈・近藤秀裕・廣野育生・平野雪・金子元・木下滋晴・小島大輔・山崎英樹・崎山一孝・辰野竜平・山根晃・新名真也・高谷智裕・荒川修・阪倉良孝
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2013-03-28
• [Presentation] Search for the genes which associate with TTX sensing of tiger puffer Takifugu rubripes by microarray (2013)
  • Author(s): Okita, K., Gotoh, K., Kondo, H., Hirono, I., Hirano, Y., Kaneko, G., Kinoshita, S., Ojima, D., Yamazaki, H., Sakiyama, K., Tatsuno, R., Yamane, H., Niina, S., Takatani, T., Arakawa, O., Sakakura, Y.
  • Organizer: 9th East China Sea Conference ~International Conference on the Marine Biodiversity and Environmental Fisheries Science of the East China Sea~
  • Place of Presentation: 国立台湾海洋大学（台湾，Keelung市）
• [Presentation] Puffers smell tetrodotoxin (2012)
  • Author(s): Okita, K., Yamasaki, H., Sakiyama, K., Yamane, H., Niina, S., Takatani, T., Arakawa, O. &Sakakura, Y.
  • Organizer: 10th Japan-Korea, Korea-Japan Joint Symposiumon Aquaculture
  • Place of Presentation: Nagasaki University
  • Year and Date: 2012-12-08
• [Book] 第7章魚類の行動と種苗生産「魚類の行動研究と水産資源管理,(棟方有宗・小林牧人・有元貴文編)」 (2013)
  • Author(s): 阪倉良孝・萩原篤志
  • Total Pages: 15
  • Publisher: 恒星社厚生閣