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Research on the molecular network implicated in the temperature acclimation of fish

Research Project

Project/Area Number 24380113
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Fisheries chemistry
Research InstitutionKitasato University

Principal Investigator

SHUGO Watabe  北里大学, 海洋生命科学部, 教授 (40111489)

Research Collaborator IKEDA Daisuke  北里大学, 海洋生命科学部, 講師
MIZUSAWA Nanami  北里大学, 海洋生命科学部, 博士研究員
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywords温度馴化 / ミオシン / 分子ネットワーク / 転写産物 / 次世代シーケンサ / メダカ / 転写因子 / 遺伝子発現 / 温度適応
Outline of Final Research Achievements

Medaka was employed to investigate the molecular network implicated in the temperature acclimation of fish. The fish were introduced into the aquarium at 24℃, and water temperature was shifted to either 10 or 30℃ within 1 day and subsequently maintained for a minimum of 30 days. Total RNAs were prepared from the fast skeletal muscles and brains of the fish acclimated and those before and just after the temperature shift. Global gene expression analysis was carried out using next generation sequencing to notify differentially expressed genes between 10- and 30℃ -acclimated fish. Differently expressed were 31 and 20 genes in the muscle and brain tissues, respectively, between the two acclimated groups. Among 31 genes in the muscle, the expression levels of those encoding heat shock protein and E3 ubiquitin ligase were found to be changed even in the fish just after the temperature shift.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (9 results)

All 2015 2014 2013 2012

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (5 results)

  • [Journal Article] Regulation of gene expression mediating indeterminate muscle growth in teleosts.2015

    • Author(s)
      A. K. Shakur Ahammad, Shigeharu Kinoshita, Md. Asaduzzaman, Shuichi Asakawa and Shugo Watabe
    • Journal Title

      Mech. Development

      Volume: in press Pages: 53-65

    • DOI

      10.1016/j.mod.2015.02.006

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of short-term cold acclimation on FoF1-ATPase activity in skeletal muscle of red seabream Pagrus major (Temminck & Schlegel).2014

    • Author(s)
      Kaneko G, Sawada A, Ushio H, Watabe S.
    • Journal Title

      Aquaculture Res

      Volume: 未定 Pages: 1-4

    • DOI

      10.1111/are.12132

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lampreys have a single gene cluster for the fast skeletal myosin heavy chain gene family2013

    • Author(s)
      Daisuke Ikeda, Yosuke Ono, Shigeki Hirano, Nobuhiro Kan-no and Shugo Watabe
    • Journal Title

      PLOS ONE

      Volume: 8 Issue: 12 Pages: e85500-e85500

    • DOI

      10.1371/journal.pone.0085500

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Global gene expression analysis of gill tissues from normal and thermally selected strains of rainbow trout.2012

    • Author(s)
      Tan, et al.
    • Journal Title

      Fish. Sci

      Volume: 78 Pages: 1041-1049

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 急激に水温変動させたメダカの筋肉のRNA-seq解析2015

    • Author(s)
      水澤奈々美・加賀谷禎子・牛島玲奈・池田大介・小山寛喜・陳 盈光・浅川修一・安元 剛・神保 充・菅野信弘・渡部終五
    • Organizer
      平成27年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学 品川キャンパス
    • Year and Date
      2015-03-27 – 2015-03-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] シーラカンス速筋型ミオシン重鎖遺伝子の機能解析2014

    • Author(s)
      池田大介・菅野信弘・渡部終五
    • Organizer
      平成26年度日本水産学会秋季大会
    • Place of Presentation
      九州大学 箱崎キャンパス
    • Year and Date
      2014-09-19 – 2014-09-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] メダカの温度馴化で発現変動する遺伝子転写産物の同定2014

    • Author(s)
      池田大介・小山寛喜・菅野信弘・陳 盈光・浅川修一・安元 剛・神保 充・渡部終五
    • Organizer
      平成26年度日本水産学会春季大会
    • Place of Presentation
      北海道大学函館キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 温度依存的に発現するメダカ遺伝子転写産物の網羅的解析2013

    • Author(s)
      渡部終五, ら
    • Organizer
      平成25年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学品川キャンパス
    • Year and Date
      2013-03-28
    • Related Report
      2012 Annual Research Report
  • [Presentation] 次世代シーケンサおよびゲノムデータベースを利用したメダカの温度馴化で発現変動する遺伝子転写産物の網羅的解析2013

    • Author(s)
      池田大介・小山寛喜・菅野信弘・陳 盈光・浅川修一・安元 剛・神保 充・渡部終五
    • Organizer
      平成25年度日本水産学会秋季大会
    • Place of Presentation
      三重大学
    • Related Report
      2013 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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