| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Outline of Final Research Achievements |
A novel method integrating hyperspectral imaging and excitation-emission matrix (EEM) data measurement was developed and the distribution of gluten and starch in dough was visualized without any pre-processing. The fluorescence images of thin section samples of gluten, starch and dough were acquired at 63 different excitation and emission wavelength conditions, resulting in a set of data which consists of the EEM data of each pixel. Each pixel in the dough sample image was evaluated by the degree of cosine similarity between the EEM of the pixel and the EEM of gluten and starch. A high value of cosine similarity to gluten or starch would mean that the particular area in dough contained much gluten or starch, respectively. Each pixel was colored according to the similarity value to create a pseudo color image showing the distribution of gluten and starch. The stained image showed roughly the same patterns as the pseudo color EEM image, thereby validating the EEM imaging method.
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