| Project/Area Number |
24380150
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied animal science
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
ASO Hisashi 東北大学, (連合)農学研究科(研究院), 教授 (50241625)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
WATANABE Koichi 東北大学, 大学院農学研究科, 助教 (80261494)
KITAZAWA Haruki 東北大学, 大学院農学研究科, 准教授 (10204885)
OKADA Natsumi 東北大学, 技術一般職員 (10621584)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
|
|---|
| Keywords | プリオン / アルドラーゼA / M細胞 / 腸管免疫 / マウス腸管上皮細胞 / ウシ腸管上皮細胞 / 侵入機構 / 抗体阻害 / 牛腸管上皮細胞株 / マウス腸管上皮細胞株 / 侵入 / トランスサイトーシス / プロテオーム解析 / 輸送小胞 / 組織解析 / aldolase A |
|---|
| Outline of Final Research Achievements |
The exact mechanism by which BSE crosses the intestinal barrier is not clear. M cells are able to incorporate large numbers of PrP coated magnetic particles into intracellular vesicles, which we collected. The results of 2-DE show a specific protein associated with the PrP-coated particles. This protein was identified as aldolase A, a glycolytic pathway enzyme, using LC-MS/MS analysis. Aldolase A was synthesized and secreted by BIE cells, and increased during M cell differentiation. In the villi of the bovine intestine, aldolase A was detected on the surface of the epithelium and in the mucus droplet of goblet cells. In the FAE of bovine jejunal and ileal Peyer’s patches, aldolase A was localized on the surface and the apical part of the M cells. The binding of rbPrP to aldolase A was clearly detected and inhibited by pre-treatment of anti-aldolase A antibody. Therefore, aldolase A-positive M cells may play a key role in the invasion of BSE into the body.
|
