Prion protein binds to aldolase A in intracellular vesicles of bovine intestinal M cells
| Project/Area Number |
24380150
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Tohoku University |
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Principal Investigator |
ASO Hisashi 東北大学, (連合)農学研究科(研究院), 教授 (50241625)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Koichi 東北大学, 大学院農学研究科, 助教 (80261494)
KITAZAWA Haruki 東北大学, 大学院農学研究科, 准教授 (10204885)
OKADA Natsumi 東北大学, 技術一般職員 (10621584)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Keywords | プリオン / アルドラーゼA / M細胞 / 腸管免疫 / マウス腸管上皮細胞 / ウシ腸管上皮細胞 / 侵入機構 / 抗体阻害 / 牛腸管上皮細胞株 / マウス腸管上皮細胞株 / 侵入 / トランスサイトーシス / プロテオーム解析 / 輸送小胞 / 組織解析 / aldolase A |
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| Outline of Final Research Achievements |
The exact mechanism by which BSE crosses the intestinal barrier is not clear. M cells are able to incorporate large numbers of PrP coated magnetic particles into intracellular vesicles, which we collected. The results of 2-DE show a specific protein associated with the PrP-coated particles. This protein was identified as aldolase A, a glycolytic pathway enzyme, using LC-MS/MS analysis. Aldolase A was synthesized and secreted by BIE cells, and increased during M cell differentiation. In the villi of the bovine intestine, aldolase A was detected on the surface of the epithelium and in the mucus droplet of goblet cells. In the FAE of bovine jejunal and ileal Peyer’s patches, aldolase A was localized on the surface and the apical part of the M cells. The binding of rbPrP to aldolase A was clearly detected and inhibited by pre-treatment of anti-aldolase A antibody. Therefore, aldolase A-positive M cells may play a key role in the invasion of BSE into the body.
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Report
(4 results)
Research Products
(36 results)
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[Journal Article] Advanced Application of Porcine Intramuscular Adipocytes for Evaluating Anti-Adipogenic and Anti-Inflammatory Activities of Immunobiotics.2015
Author(s)
Masahiko Suzuki, Asuka Tada, Paulraj Kanmani, Hitoshi Watanabe, Hisashi Aso, Yoshihito Suda, Tomonori Nochi, Kenji Miyazawa, Kazutoyo Yoda, Fang He, Masataka Hosoda, Tadao Saito, Julio Villena, Haruki Kitazawa
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Journal Title
PLoS ONE
Volume: 10 (3)
Issue: 3
Pages: 1-20
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Myostatin alters glucose transporter-4 (GLUT4) expression in bovine skeletal muscles and myoblasts isolated from double muscled (DM) and normal muscled (NM) Japanese shorthorn cattle.2014
Author(s)
H. Takahashi, K. Sato, T. Yamaguchi, M. Miyake, H. Watanabe, Y. Nagasawa, E. Kitagawa, S. Terada, M. Urakawa, M. T. Rose, C. D. McMahon, K. Watanabe, S. Ohwada, T. Gotoh, H. Aso
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Journal Title
Domestic Animal Endocrinology
Volume: 48
Pages: 62-68
DOI
Related Report
Peer Reviewed / Open Access
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