| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Outline of Final Research Achievements |
It was aimed to produce genetically designed calves efficiently using in vitro fertilization techniques of gametes from the parents which were accurately analyzed for whole genomes. The blood samples of 110 females from the herd of the Tochigi Prefectural Livestock & Dairy Experimental Center were highly accurately analyzed for single nucleotide polymorphism (SNP) of genotypes on economically valuable genes. The genotypes of same genes were also determined for frozen semen samples purchased of ten bulls. Based on the genotyping results, combinations of ova and sperm were planned for yields of genetically superior calves. Ova were obtained from ovaries of the females of the center using OPU technics or ovaries from the slaughterhouse in Utsunomiya-city. The resulting blastocysts, which should have the desired genotypes, were obtained using the previously reported IVM-IVF-IVC system. The produced blastocysts were transferred to recipient females, but no calf was obtained until now.
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