• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study on a regulatory mechanism of follicular growth in goats by applying optogenetic technology

Research Project

Project/Area Number 24380154
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Applied animal science
Research InstitutionNagoya University

Principal Investigator

Ohkura Satoshi  名古屋大学, 生命農学研究科, 教授 (20263163)

Co-Investigator(Kenkyū-buntansha) MATSUDA Fuko  東京大学, 大学院農学生命科学研究科, 准教授 (10608855)
Co-Investigator(Renkei-kenkyūsha) FURUSAWA Tadashi  農業・食品産業技術総合研究機構, 畜産研究部門, 主任研究員 (00343997)
OHKOSHI Katsuhiro  農業・食品産業技術総合研究機構, 畜産研究部門, 主任研究員 (00414889)
Research Collaborator KOBAYASHI Kenta  
SUETOMI Yuta  
TATEBAYASHI Ryoki  
OKUDA Kazuhiro  
KATO Masahiro  
HASSANEEN Ahmed Saad Ahmed  
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Keywords応用動物 / 神経科学 / 獣医学 / 畜産学 / GnRH / ヤギ / キスペプチン / ニューロキニンB
Outline of Final Research Achievements

The present study aimed to elucidate the hypothalamic mechanism controlling the follicular growth in ruminants. First, the genome structure of the goat neurokinin B gene (TAC3) and a transcriptional regulatory domain of TAC3 were identified. Second, to generate genetically modified goats which could control the activity of KNDy neurons (neurons contain neurokinin B) in the hypothalamic arcuate nucleus by using an optogenetic technology, we applied the gene introduction method by the adeno-associated virus vector and Cre-loxP system in goats to establish a KNDy neuron-specific expression of light-responsive ion channels. Third, we demonstrated the localization of KNDy neurons in the hypothalamic arcuate nucleus in cows by immunohistochemistry and suggested that KNDy neurons have a key role to stimulate gonadotropin-releasing hormone (GnRH) secretion, and then follicular growth in ruminants.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (63 results)

All 2016 2015 2014 2013 2012 Other

All Journal Article (18 results) (of which Peer Reviewed: 17 results,  Acknowledgement Compliant: 2 results,  Open Access: 3 results) Presentation (43 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Book (2 results)

  • [Journal Article] The Luteinising Hormone Surge-Generating System is Functional in Male Goats as in Females: Involvement of Kisspetin Neurons in the Medial Preoptic Area2015

    • Author(s)
      F. Matsuda, K. Nakatsukasa, Y. Suetomi, Y. Naniwa, D. Ito, N. Inoue, Y. Wakabayashi, H. Okamura, K.-I. Maeda, Y. Uenoyama, H. Tsukamura and S. Ohkura
    • Journal Title

      Journal of Neuroendocrinology

      Volume: 27 Issue: 1 Pages: 57-65

    • DOI

      10.1111/jne.12235

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Effects of intravenous administration of neurokinin receptor subtype-selective agonists on gonadotropin-releasing hormone pulse generator activity and luteinizing hormone secretion in goats2015

    • Author(s)
      Yamamura T, Wakabayashi Y, Ohkura S, Navarro VM, Okamura H
    • Journal Title

      Journal of Reproduction and Development

      Volume: 61 Issue: 1 Pages: 20-29

    • DOI

      10.1262/jrd.2014-109

    • NAID

      130004700243

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structure-activity relationship study on senktide for development of novel potent neurokinin-3 receptor selective agonists.2015

    • Author(s)
      Misu R, Yamamoto K, Yamada A, Noguchi T, Ohno H, Yamamura T, Okamura H, Matsuda F, Ohkura S, Oishi S, Fujii N.
    • Journal Title

      MedChemCommun

      Volume: 6 Issue: 3 Pages: 469-476

    • DOI

      10.1039/c4md00514g

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Differential changes in luteinizing hormone secretion after administration of the investigational metastin/kisspeptin analog TAK-683 in goats.2015

    • Author(s)
      Endo, N., Tamesaki, C., Ohkura, S., Wakabayashi, Y., Matsui, H., Tanaka, A., Watanabe, T., Okamura, H. and Tanaka, T.
    • Journal Title

      Animal Reproduction Science

      Volume: 159 Pages: 87-93

    • DOI

      10.1016/j.anireprosci.2015.05.016

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ovarian and hormonal responses to follicular phase administration of investigational metastin/kisspeptin analog, TAK-683, in goats.2014

    • Author(s)
      Goto, Y., Endo, N., Nagai, K., Ohkura, S., Wakabayashi, Y., Tanaka, A., Matsui, H., Kusaka, M., Okamura, H. and Tanaka, T.
    • Journal Title

      Reproduction in Domestic Animals

      Volume: 49 Issue: 2 Pages: 338-342

    • DOI

      10.1111/rda.12283

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] <i>KISS1</i> Gene Expression in the Developing Brain of Female Pigs in Pre- and Peripubertal Periods2014

    • Author(s)
      Ieda, N., Uenoyama, Y., Tajima, Y., Nakata, T., Kano, M., Naniwa, Y., Watanabe, Y., Minabe, S., Tomikawa, J., Inoue, N., Matsuda, F., Ohkura, S., Maeda, K.-I. and Tsukamura, H.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 60 Issue: 4 Pages: 312-316

    • DOI

      10.1262/jrd.2013-129

    • NAID

      130004137827

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of novel neurokinin 3 receptor (NK3R) selective agonists with resistance to proteolytic degradation.2014

    • Author(s)
      Misu R, Oishi S, Yamada A, Yamamura T, Matsuda F, Yamamoto K, Noguchi T, Ohno H, Okamura H, Ohkura S, Fujii N.
    • Journal Title

      J. Med. Chem.

      Volume: 57 Issue: 20 Pages: 8646-8651

    • DOI

      10.1021/jm500771w

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Oestrogen-Induced Activation of Preoptic Kisspeptin Neurones May be Involved in the Luteinising Hormone Surge in Male and Female Japanese Monkeys2014

    • Author(s)
      Watanabe Y, Uenoyama Y, Suzuki J, Takase K, Suetomi Y, Ohkura S, Inoue N, Maeda KI. and Tsukamura H
    • Journal Title

      Journal of Neuroendocrinology

      Volume: 26 Issue: 12 Pages: 909-917

    • DOI

      10.1111/jne.12227

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The effects of chronic subcutaneous administration of an investigational kisspeptin analogue, TAK-683, on gonadotropin-releasing hormone pulse generator activity in goats.2014

    • Author(s)
      Yamamura, T., Wakabayashi, Y., Sakamoto, K., Matsui, H., Kusaka, M., Tanaka, T., Ohkura, S. and Okamura, H.
    • Journal Title

      Neuroendocrinology

      Volume: 100 Issue: 2-3 Pages: 250-264

    • DOI

      10.1159/000369819

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] プロスタグランジンF2α及び性腺刺激ホルモンによる排卵同期化法を用いた豚の定時人工授精.2014

    • Author(s)
      中田智子, 田島瑶子, 野口倫子, 上野山賀久, 大蔵 聡, 前多敬一郎, 鈴木千恵, 吉岡耕治
    • Journal Title

      日本獣医師会雑誌

      Volume: 67 Pages: 119-124

    • NAID

      130004640744

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Cloning and Identification of the Transcriptional Regulatory Domain of the Goat Neurokinin B Gene <i>TAC3</i>2013

    • Author(s)
      Suetomi, Y., Matsuda, F., Uenoyama, Y., Maeda, K.-I., Tsukamura, H. and Ohkura, S.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 59 Issue: 5 Pages: 463-469

    • DOI

      10.1262/jrd.2013-037

    • NAID

      130003361048

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Full-Length Kisspeptin Administration on Follicular Development in Japanese Black Beef Cows2013

    • Author(s)
      Naniwa, Y., Nakatsukasa, K., Setsuda, S., Oishi, S., Fujii, N., Matsuda, F., Uenoyama, Y., Tsukamura, H., Maeda, K. -I. and Ohkura, S.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 59 Issue: 6 Pages: 588-594

    • DOI

      10.1262/jrd.2013-064

    • NAID

      130003381852

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential Effects of Continuous Exposure to the Investigational Metastin/Kisspeptin Analog TAK-683 on Pulsatile and Surge Mode Secretion of Luteinizing Hormone in Ovariectomized Goats2013

    • Author(s)
      Tanaka, T., Ohkura, S., Wakabayashi, Y., Kuroiwa, T., Nagai, K., Endo, N., Tanaka, A., Matsui, H., Kusaka, M. and Okamura, H.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 59 Issue: 6 Pages: 563-568

    • DOI

      10.1262/jrd.2013-060

    • NAID

      130003361063

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of peripherally administered kisspeptin-10 on GnRH neurosecretion into the hypophyseal portal circulation in ovariectomized goat does.2012

    • Author(s)
      Tanaka, T., Ohkura, S., Wakabayashi, Y. and Okamura, H.
    • Journal Title

      Small Ruminant Research

      Volume: 105 Issue: 1-3 Pages: 273-276

    • DOI

      10.1016/j.smallrumres.2012.01.007

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Central Administration of Neurokinin B Activates Kisspeptin/NKB Neurons in the Arcuate Nucleus and Stimulates Luteinizing Hormone Secretion in Ewes During the Non-Breeding Season2012

    • Author(s)
      Sakamoto K, Murata K, Wakabayashi Y, Yayou K, Ohkura S, Takeuchi Y, Mori Y, Okamura H.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 58 Issue: 6 Pages: 700-706

    • DOI

      10.1262/jrd.2011-038

    • NAID

      10031146934

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Central distribution of kiss2 neurons and peri-pubertal changes in their expression in the brain of male and female red seabream Pagrus major.2012

    • Author(s)
      Shimizu, Y, Tomikawa, J., Hirano, K., Nanikawa, Y., Akazome, Y., Kanda, S., Kazeto, Y., Okuzawa, K., Uenoyama, Y., Ohkura, S., Tsukamura, H., Maeda, K.-I., Gen, K, Oka, Y. and Yamamoto, N.
    • Journal Title

      General and Comparative Endocrinology

      Volume: 175 Pages: 432-442

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Follicular Growth and Atresia in Mammalian Ovaries: Regulation by Survival and Death of Granulosa Cells2012

    • Author(s)
      Matsuda, F., Inoue, N., Manabe, N. and Ohkura, S.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 58 Issue: 1 Pages: 44-50

    • DOI

      10.1262/jrd.2011-012

    • NAID

      10030407021

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 繁殖障害の根本治療に向けての新たな方法論 : KNDyニューロンの応用.2012

    • Author(s)
      前多敬一郎・大蔵聡・松田二子・難波陽介・若林嘉浩・岡村裕昭・井上直子・上野山賀久・東村博子
    • Journal Title

      家畜診療

      Volume: 59 Pages: 387-393

    • NAID

      40019379912

    • Related Report
      2012 Annual Research Report
  • [Presentation] ウシGnRHニューロン不死化細胞株の樹立2016

    • Author(s)
      尾崎理穂・末富祐太・松山秀一・木村康二・大蔵 聡・松田二子
    • Organizer
      日本畜産学会第121回大会
    • Place of Presentation
      東京都武蔵野市
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Electrophysiological technique for monitoring the hypothalamic mechanism regulating pulsatile GnRH release in goats.2015

    • Author(s)
      Ohkura, S., Matsuda, F., Wakabayashi, Y., Yamamura, T. and Okamura, H.
    • Organizer
      International Conference on Biology and Pathology of Reproduction in Domestic Animals
    • Place of Presentation
      Gdansk, Poland
    • Year and Date
      2015-09-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular cloning and identification of the transcriptional regulatory domain of the goat neurokinin B gene TAC3.2015

    • Author(s)
      Suetomi, Y., Matsuda, F., Uenoyama, Y., Tsukamura, H., Maeda, K.-I. and Ohkura, S.
    • Organizer
      International Conference on Biology and Pathology of Reproduction in Domestic Animals
    • Place of Presentation
      Gdansk, Poland
    • Year and Date
      2015-09-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] アデノ随伴ウイルス(AAV)ベクターを用いたヤギ視床下部神経細胞への遺伝子導入法の検討2015

    • Author(s)
      末富祐太・奥田雄大・小林憲太・大蔵 聡・松田二子
    • Organizer
      第108回日本繁殖生物学会大会
    • Place of Presentation
      宮崎県宮崎市
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] TALENを用いたシバヤギ体細胞のKISS1遺伝子改変2015

    • Author(s)
      舘林亮輝・佐久間哲史・山本 卓・大蔵 聡・松田二子
    • Organizer
      第108回日本繁殖生物学会大会
    • Place of Presentation
      宮崎県宮崎市
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] 弓状核へのNeurokinin B局所投与により誘起された神経活動上昇は反対側の弓状核キスペプチンニューロン群へ伝達される2015

    • Author(s)
      若林嘉浩・山村 崇・大蔵 聡・岡村裕昭
    • Organizer
      第108回日本繁殖生物学会大会
    • Place of Presentation
      宮崎県宮崎市
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] ニューロキニン作動薬を用いた新たな卵胞発育制御技術の開発2015

    • Author(s)
      山村 崇・若林嘉浩・三須良介・大石真也・大蔵 聡・藤井信孝・岡村裕昭
    • Organizer
      日本畜産学会第119回大会
    • Place of Presentation
      栃木県宇都宮市
    • Year and Date
      2015-03-27 – 2015-03-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] 新規ニューロキニン-3受容体アゴニストの創製と繁殖中枢制御への応用2015

    • Author(s)
      山本昂輝・大石真也・三須良介・山田 愛・野口太朗・大野浩章・山村 崇・岡村裕昭・松田二子・大蔵 聡・藤井信孝
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2015-03-25 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Development of novel neurokinin-3 receptor selective agonists with registance against proteolytic degradation.2014

    • Author(s)
      Misu, R., Oishi, S., Yamada, A., Yamamoto, K., Noguchi, T., Ohno, H., Yamamura, T., Okamura, H., Matsuda, F., Ohkura, S. and Fujii, N.
    • Organizer
      The 51st Japanese Peptide Symposium
    • Place of Presentation
      Tokushima, Japan
    • Year and Date
      2014-10-22 – 2014-10-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Establishment of immortalized neuronal cell lines derived from fetal goat hypothalamus.2014

    • Author(s)
      Suetomi, Y., Tsukamura, H., Ohkura, S. and Matsuda, F.
    • Organizer
      The 3rd World Congress on Reproductive Biology
    • Place of Presentation
      Edinburgh, UK
    • Year and Date
      2014-09-02 – 2014-09-04
    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
  • [Presentation] Development of novel neurokinin-3 receptor agonists that potently stimulate the GnRH pulse generator.2014

    • Author(s)
      Yamamura, T., Misu, R., Wakabayashi, Y., Ohkura, S., Oishi, S., Fujii, N. and Okamura, H.
    • Organizer
      The 3rd World Congress on Reproductive Biology
    • Place of Presentation
      Edinburgh, UK
    • Year and Date
      2014-09-02 – 2014-09-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] Differential changes in LH secretion after administration of investigational metastin/kisspeptin analog TAK-683 to goats.2014

    • Author(s)
      Tamesaki, C., Endo, N., Ohkura, S., Wakabayashi, Y., Matsui, H., Tanaka, A., Watanabe, T., Okamura, H. and Tanaka, T.
    • Organizer
      The 3rd World Congress on Reproductive Biology
    • Place of Presentation
      Edinburgh, UK
    • Year and Date
      2014-09-02 – 2014-09-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] Endocrine responses and ovarian dynamics in goats treated with low dose of investigational metastin/kisspeptin analog TAK-683 in follicular phase.2014

    • Author(s)
      Gomi, N., Endo, N., Ohkura, S., Wakabayashi, Y., Matsui, H., Matsumoto, H., Ishikawa, K., Tanaka, A., Watanabe, T., Okamura, H. and Tanaka, T.
    • Organizer
      The 3rd World Congress on Reproductive Biology
    • Place of Presentation
      Edinburgh, UK
    • Year and Date
      2014-09-02 – 2014-09-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] Establishment of immortalized neuronal cell lines derived from domestic ruminant hypothalamus.2014

    • Author(s)
      Suetomi, Y., Kato, M., Matsuyama, S., Kimura, K., Tsukamura, H., Ohkura, S. and Matsuda, F.
    • Organizer
      9th International Ruminant Reproduction Symposium
    • Place of Presentation
      Obihiro, Japan
    • Year and Date
      2014-08-25 – 2014-08-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] ニューロキニンB作動薬の末梢投与は黒毛和種雌ウシの黄体形成ホルモン分泌および卵胞発育を促進する2014

    • Author(s)
      加藤雅大・末富祐太・伊藤太祐・佐々木拓弥・難波陽介・三須良介・大石真也・藤井信孝・松田二子・大蔵 聡
    • Organizer
      第107回日本繁殖生物学会大会
    • Place of Presentation
      北海道帯広市
    • Year and Date
      2014-08-21 – 2014-08-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] アデノ随伴ウイルス(AAV)ベクターを用いたシバヤギ視床下部への遺伝子導入法の検討2014

    • Author(s)
      奥田雄大・松田二子・末富祐太・小林憲太・大蔵 聡
    • Organizer
      第107回日本繁殖生物学会大会
    • Place of Presentation
      北海道帯広市
    • Year and Date
      2014-08-21 – 2014-08-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] シバヤギ弓状核キスペプチンニューロン分布領域へのNeurokinin B局所投与がGnRHパルス産生におよぼす影響2014

    • Author(s)
      若林嘉浩・山村 崇・大蔵 聡・岡村裕昭
    • Organizer
      第107回日本繁殖生物学会大会
    • Place of Presentation
      北海道帯広市
    • Year and Date
      2014-08-21 – 2014-08-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] タキキニンファミリーの神経ペプチドがGnRHパルスジェネレータにおよぼす影響2014

    • Author(s)
      山村 崇・若林嘉浩・大蔵 聡・岡村裕昭
    • Organizer
      第107回日本繁殖生物学会大会
    • Place of Presentation
      北海道帯広市
    • Year and Date
      2014-08-21 – 2014-08-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] ペプチダーゼ耐性を有する新規NK3受容体アゴニストの創製研究2014

    • Author(s)
      三須良介・大石真也・山田 愛・山本昂輝・野口太朗・大野浩章・山村 崇・岡村裕昭・松田二子・大蔵 聡・藤井信孝
    • Organizer
      日本ケミカルバイオロジー学会第9回年会
    • Place of Presentation
      大阪府豊中市
    • Year and Date
      2014-06-11 – 2014-06-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] NK3受容体アゴニストの生体内安定性評価と新規誘導体の創製2014

    • Author(s)
      三須良介・大石真也・山田 愛・山本昂輝・野口太朗・大野浩章・山村 崇・岡村裕昭・松田二子・大蔵 聡・藤井信孝
    • Organizer
      第11回GPCR研究会
    • Place of Presentation
      東京都江東区
    • Year and Date
      2014-05-09 – 2014-05-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] Establishment of immortalized neuronal cell lines derived from domestic ruminant hypothalamus.2014

    • Author(s)
      Suetomi, Y., Kato, M., Matsuyama, S., Kimura, K., Tsukamura, H., Ohkura, S. and Matsuda, F.
    • Organizer
      The 9th International Ruminant Reproduction Symposium
    • Place of Presentation
      Obihiro, Japan
    • Related Report
      2013 Annual Research Report
  • [Presentation] Cloning of full-length mRNA and 5’-upstream DNA sequences of goat TAC3 and the analysis of its transcriptional regulatory mechanisms.2013

    • Author(s)
      Suetomi, Y., Matsuda, F., Uenoyama, Y., Tsukamura, H., Maeda, K.-I. and Ohkura, S.
    • Organizer
      The 46th Annual Meeting of the Society for the Study of Reproduction
    • Place of Presentation
      Montreal, Canada
    • Related Report
      2013 Annual Research Report
  • [Presentation] Generation of immortalized neuronal cell lines derived from bovine hypothalamic arcuate nucleus and medial preoptic area.2013

    • Author(s)
      Matsuda, F., Suetomi, Y., Hassaneen, A.S.A., Kato, M., Matsuyama, S., Kimura, K. and Ohkura, S.
    • Organizer
      The 46th Annual Meeting of the Society for the Study of Reproduction
    • Place of Presentation
      Montreal, Canada
    • Related Report
      2013 Annual Research Report
  • [Presentation] Kisspeptin analog, TAK-683, has a potent bioactivity to stimulate follicular development and luteinizing hormone secretion in Japanese Black beef cows.2013

    • Author(s)
      Naniwa, Y., Nakatsukasa, K., Suetomi, Y., Matsuda, F., Matsui, H., Kusaka, M., Ohtaki, T., Tanaka, T., Okamura, H. and Ohkura, S.
    • Organizer
      The 46th Annual Meeting of the Society for the Study of Reproduction
    • Place of Presentation
      Montreal, Canada
    • Related Report
      2013 Annual Research Report
  • [Presentation] Structure-activity relationship study of tachykinin peptides for development of novel neurokinin-3 receptor selective agonists.2013

    • Author(s)
      Misu, R., Oishi, S., Noguchi, T., Yamada, A., Ohno, H., Matsuda, F., Ohkura, S. and Fujii, N.
    • Organizer
      The 23rd American Peptide Symposium
    • Place of Presentation
      Waikoloa, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] 胎仔シバヤギ視床下部由来不死化神経細胞株の樹立2013

    • Author(s)
      末富祐太・松田二子・上野山賀久・前多敬一郎・束村博子・大蔵 聡
    • Organizer
      第106回日本繁殖生物学会大会
    • Place of Presentation
      東京都府中市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 新規κオピオイド受容体拮抗剤の末梢投与がシバヤギGnRHパルスジェネレーター活動におよぼす効果2013

    • Author(s)
      伊藤太祐・中務桂佑・若林嘉浩・山村 崇・岡村裕昭・大石真也・野口太朗・藤井信孝・上野山賀久・束村博子・前多敬一郎・松田二子・大蔵 聡
    • Organizer
      第106回日本繁殖生物学会大会
    • Place of Presentation
      東京都府中市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 卵胞期のウシにおけるキスペプチン末梢投与が性腺刺激ホルモン分泌、発情行動および排卵におよぼす効果の検討2013

    • Author(s)
      難波陽介・Ahmed Saad Ahmed Hassaneen・加藤雅大・伊藤太祐・奥田雄大・末富祐太・佐々木拓弥・説田章平・大石真也・藤井信孝・上野山賀久・束村博子・前多敬一郎・松田二子・大蔵 聡
    • Organizer
      第106回日本繁殖生物学会大会
    • Place of Presentation
      東京都府中市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Evaluation of biological stability of neurokinin-3 receptor agonists for the development of novel biostable analogs.2013

    • Author(s)
      Misu, R., Oishi, S., Yamada, A., Noguchi, T., Ohno, H., Matsuda, F., Ohkura, S. and Fujii, N.
    • Organizer
      The 50th Japanese Peptide Symposium
    • Place of Presentation
      Suita, Japan
    • Related Report
      2013 Annual Research Report
  • [Presentation] Generation of immortalized neuronal cell lines derived from goat hypothalamic arcuate nucleus and medial preoptic area.2012

    • Author(s)
      Matsuda, F., Suetomi, Y., Uenoyama, Y., Maeda, K.-I., Tsukamura, H. and Ohkura, S.
    • Organizer
      The 2nd World Conference on Kisspeptin Signaling in the Brain
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2012-11-08
    • Related Report
      2012 Annual Research Report
  • [Presentation] Structure-activity relationship study of tachykinin peptides for development of novel neurokinin-3 receptor agonists.2012

    • Author(s)
      Misu, R., Oishi, S., Noguchi, T., Yamada, A., Ohno, H., Yamamura, T., Okamura, H., Matsuda, F., Ohkura, S. and Fujii, N.
    • Organizer
      The 2nd World Conference on Kisspeptin Signaling in the Brain
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2012-11-08
    • Related Report
      2012 Annual Research Report
  • [Presentation] Cloning of goat TAC3 upstream region and evaluation of its promoter activity.2012

    • Author(s)
      Suetomi, Y., Matsuda, F., Uenoyama, Y., Tsukamura, H., Maeda, K.-I. and Ohkura, S.
    • Organizer
      The 2nd World Conference on Kisspeptin Signaling in the Brain
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2012-11-07
    • Related Report
      2012 Annual Research Report
  • [Presentation] Continuous administration of senktide, a neurokinin B receptor agonist, facilitates intermittent activities of the GnRH pulse generator for 24 h in goats.2012

    • Author(s)
      Yamamura, T., Wakabayashi, Y., Fujii, N., Oishi, S., Ohkura, S. and Okamura, H.
    • Organizer
      The 2nd World Conference on Kisspeptin Signaling in the Brain
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2012-11-07
    • Related Report
      2012 Annual Research Report
  • [Presentation] キスペプチンノKNDyニューロンを応用した新たな繁殖制御技術の可能性.2012

    • Author(s)
      大蔵聡
    • Organizer
      第23回牛学術セミナー
    • Place of Presentation
      岡山県岡山市、他5ヵ所(招待講演)
    • Year and Date
      2012-10-25
    • Related Report
      2012 Annual Research Report
  • [Presentation] NK3受容体リガンドの安定性評価と新規選択的アゴニストの創製.2012

    • Author(s)
      山田愛・三須良介・大石真也・大野浩章・松田二子・大蔵聡・藤井信孝
    • Organizer
      第62回日本薬学会近畿支部総会・大会
    • Place of Presentation
      兵庫県西宮市
    • Year and Date
      2012-10-20
    • Related Report
      2012 Annual Research Report
  • [Presentation] Analysis of the transcriptional regulatory mechanism of neurokinin B gene, TAC3, in the goat.2012

    • Author(s)
      Matsuda, F., Suetomi, Y., Maeda, K.-I., Tsukamura, H. and Ohkura, S.
    • Organizer
      Society for Neuroscience 42nd Annual Meeting
    • Place of Presentation
      New Orleans, U.S.A.
    • Year and Date
      2012-10-13
    • Related Report
      2012 Annual Research Report
  • [Presentation] Senktide, a neurokinin B receptor agonist, stimulates pulsatile LH secretion through a mechanism mediated by the GnRH pulse generator in goats.2012

    • Author(s)
      Wakabayashi, Y., Yamamura, T., Ohkura, S., Homma, T., Sakamoto, K., Mori, Y. and Okamura, H.
    • Organizer
      Society for Neuroscience 42nd Annual Meeting
    • Place of Presentation
      New Orleans, U.S.A.
    • Year and Date
      2012-10-13
    • Related Report
      2012 Annual Research Report
  • [Presentation] シバヤギ視床下部初代培養細胞の不死化と神経由来細胞クローンの同定.2012

    • Author(s)
      松田二子・末富祐太・上野山賀久・前多敬一郎・束村博子・大蔵聡
    • Organizer
      第105回日本繁殖生物学会大会
    • Place of Presentation
      茨城県つくば市
    • Year and Date
      2012-09-07
    • Related Report
      2012 Annual Research Report
  • [Presentation] シバヤギにおけるM0遺伝子上流域の同定とそのプロモーター活性の評価.2012

    • Author(s)
      末富祐太・松田二子・上野山賀久・東村博子・前多敬一郎・大蔵聡
    • Organizer
      第105回日本繁殖生物学会大会
    • Place of Presentation
      茨城県つくば市
    • Year and Date
      2012-09-07
    • Related Report
      2012 Annual Research Report
  • [Presentation] キスペプチン類縁体TAK-683の末梢投与はウシの卵胞発育および黄体形成ホルモン分泌を刺激する.2012

    • Author(s)
      難波陽介・中務桂佑・末富祐太・松田二子・松井久典・日下雅美・大瀧徹也・田中知己・岡村裕昭・大蔵聡
    • Organizer
      第105回日本繁殖生物学会大会
    • Place of Presentation
      茨城県つくば市
    • Year and Date
      2012-09-07
    • Related Report
      2012 Annual Research Report
  • [Presentation] NKBは弓状核キスペプチン/NKBニューロンに作用してGnRHパルスを産生させる.2012

    • Author(s)
      若林嘉浩・山村 崇・大蔵聡・本間玲実・坂本光平・森裕司・岡村裕昭
    • Organizer
      第105回日本繁殖生物学会大会
    • Place of Presentation
      茨城県つくば市
    • Year and Date
      2012-09-06
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヤギ視床下部視索前野および弓状核に由来する不死化神経細胞株の作出.2012

    • Author(s)
      松田二子・末富祐太・上野山賀久・前多敬一郎・東村博子・大蔵聡
    • Organizer
      日本下垂体研究会第27回学術集会
    • Place of Presentation
      山形県天童市
    • Year and Date
      2012-08-10
    • Related Report
      2012 Annual Research Report
  • [Presentation] Neuroendocrine mechanisms regulating reproductive functions in domestic ruminants.

    • Author(s)
      Ohkura, S.
    • Organizer
      National Seminar HPTI, PEI and PFI East Java 2014
    • Place of Presentation
      Surabaya, Indonesia
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Book] Kisspeptin Signaling in Reproductive Biology2013

    • Author(s)
      Okamura, H., Tsukamura, H., Ohkura, S., Uenoyama, Y., Wakabayashi, Y. and Maeda, K.-I.
    • Total Pages
      514
    • Publisher
      Springer
    • Related Report
      2013 Annual Research Report
  • [Book] 繁殖生物学2013

    • Author(s)
      大蔵 聡
    • Total Pages
      328
    • Publisher
      インターズー
    • Related Report
      2013 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi