Diversification of gene activation system by acquisition of species-specific non-coding RNAs through insertion/deletion and mutation
| Project/Area Number |
24380158
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Kyushu University (2013-2014)
Kyoto University (2012) |
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Principal Investigator |
IMAMURA TAKUYA 九州大学, 医学(系)研究科(研究院), 助教 (90390682)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Keywords | ノンコーディングRNA / DNAメチル化 / DNA脱メチル化 / 遺伝子活性化 / 生物多様性 / 進化 / エピゲノム / ノンコーディング / 霊長類 / 非コードRNA / 次世代シーケンサー / 非コードＲＮＡ / ＤＮＡメチル化 |
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| Outline of Final Research Achievements |
We performed directional RNA-seq analysis of mouse and chimpanzee tissue samples. Focusing on transcription start sites (TSSs) of protein-coding genes revealed that a significant fraction of them contain switching-points that separate antisense- and sense-biased transcription, suggesting that head-to-head transcription is more prevalent than previously thought. More than 90% of head-to-head type promoters contain CpG islands. Moreover, CCG and CGG repeats are significantly enriched in the upstream regions and downstream regions, respectively, of TSSs located in head-to-head type promoters. Genes with tissue-specific promoter-associated ncRNAs (pancRNAs) show a positive correlation between the expression of their pancRNA and mRNA, which is in accord with the proposed role of pancRNA in facultative gene activation, whereas genes with constitutive expression generally lack pancRNAs. We show here that pancRNA regulates tissue-specific gene expression in a species-dependent manner.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Identification of hypothalamic arcuate nucleus-specific enhancer region of kiss1 gene in mice.2015
Author(s)
Goto, T., Tomikawa, J., Ikegami, K., Minabe, S., Abe, H., Fukanuma, T., Imamura, T., Takase, K., Sanbo, M., Tomita, K., Hirabayashi, M., Maeda, K., Tsukamura, H., Uenoyama, Y.
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Journal Title
Mol Endocrinol
Volume: 29
Issue: 1
Pages: 121-129
DOI
Related Report
Peer Reviewed / Open Access
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