• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Diversification of gene activation system by acquisition of species-specific non-coding RNAs through insertion/deletion and mutation

Research Project

Project/Area Number 24380158
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionKyushu University (2013-2014)
Kyoto University (2012)

Principal Investigator

IMAMURA TAKUYA  九州大学, 医学(系)研究科(研究院), 助教 (90390682)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
KeywordsノンコーディングRNA / DNAメチル化 / DNA脱メチル化 / 遺伝子活性化 / 生物多様性 / 進化 / エピゲノム / ノンコーディング / 霊長類 / 非コードRNA / 次世代シーケンサー / 非コードRNA / DNAメチル化
Outline of Final Research Achievements

We performed directional RNA-seq analysis of mouse and chimpanzee tissue samples. Focusing on transcription start sites (TSSs) of protein-coding genes revealed that a significant fraction of them contain switching-points that separate antisense- and sense-biased transcription, suggesting that head-to-head transcription is more prevalent than previously thought. More than 90% of head-to-head type promoters contain CpG islands. Moreover, CCG and CGG repeats are significantly enriched in the upstream regions and downstream regions, respectively, of TSSs located in head-to-head type promoters. Genes with tissue-specific promoter-associated ncRNAs (pancRNAs) show a positive correlation between the expression of their pancRNA and mRNA, which is in accord with the proposed role of pancRNA in facultative gene activation, whereas genes with constitutive expression generally lack pancRNAs. We show here that pancRNA regulates tissue-specific gene expression in a species-dependent manner.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (30 results)

All 2015 2014 2013 2012 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (18 results) Book (3 results) Remarks (4 results)

  • [Journal Article] Gene activation-associated long noncoding RNAs function in mouse preimplantation development2015

    • Author(s)
      Hamazaki N, Uesaka M, Nakashima K, Agata K, Imamura T
    • Journal Title

      Development

      Volume: 142 Pages: 910-920

    • DOI

      10.1242/dev.116996

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of hypothalamic arcuate nucleus-specific enhancer region of kiss1 gene in mice.2015

    • Author(s)
      Goto, T., Tomikawa, J., Ikegami, K., Minabe, S., Abe, H., Fukanuma, T., Imamura, T., Takase, K., Sanbo, M., Tomita, K., Hirabayashi, M., Maeda, K., Tsukamura, H., Uenoyama, Y.
    • Journal Title

      Mol Endocrinol

      Volume: 29 Issue: 1 Pages: 121-129

    • DOI

      10.1210/me.2014-1289

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Epigenetic setting and reprogramming for neural cell fate determination and differentiation.2014

    • Author(s)
      Takuya Imamura, Masahiro Uesaka, Kinichi Nakashima
    • Journal Title

      Philosophical transactions of the Royal Society of London. Series B

      Volume: 369 Issue: 1652 Pages: 1652-1652

    • DOI

      10.1098/rstb.2013.0511

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bidirectional promoters are the major source of gene activation-associated non-coding RNAs in mammals.2014

    • Author(s)
      Uesaka M, Nishimura O, Go Y, Nakashima K, Agata K, Imamura T.
    • Journal Title

      BMC Genomics

      Volume: 15 Issue: 1 Pages: 35-35

    • DOI

      10.1186/1471-2164-15-35

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic regulation of Kissl gene expression mediating estrogen-positive feedback action in the mouse brain.2012

    • Author(s)
      富川順子
    • Journal Title

      Proceedings of the National Academy of Sciences USA (PNAS)

      Volume: 109 Issue: 20 Pages: 1294-1301

    • DOI

      10.1073/pnas.1114245109

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Bidirectional promoter-derived antisense noncoding RNA epigenetically regulates irreversible differentiation of PC12 cells2015

    • Author(s)
      Naoki Yamamoto, Kiyokazu Agata, Kinichi Nakashima, Takuya Imamura
    • Organizer
      第8回神経発生討論会
    • Place of Presentation
      九州大学病院キャンパス(福岡市)
    • Year and Date
      2015-03-19 – 2015-03-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] Species-specific repertories of promoter-associated non-coding RNAs may contribute to the diversification of gene expression profile2015

    • Author(s)
      Masahiro Uesaka, Kiyokazu Agata, Kinichi Nakashima, Takuya Imamura
    • Organizer
      第8回神経発生討論会
    • Place of Presentation
      九州大学病院キャンパス(福岡市)
    • Year and Date
      2015-03-19 – 2015-03-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] 長鎖ncRNAによるほ乳類エピゲノム制御2014

    • Author(s)
      今村拓也
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道大学高等教育推進機構(札幌市)
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] マウス初期胚の全能性を支える遺伝子を活性化するノンコーディングRNAの同定2014

    • Author(s)
      濵崎伸彦, 上坂将弘, 中島欽一, 阿形清和, 今村拓也
    • Organizer
      第107回日本繁殖生物学会大会
    • Place of Presentation
      帯広畜産大学(帯広市)
    • Year and Date
      2014-08-21 – 2014-08-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Potential role for species-specific promoter-associated non-coding RNAs in the diversification of mammalian brain2014

    • Author(s)
      Masahiro Uesaka, Osamu Nishimura, Kinichi Nakashima, Kiyokazu Agata, Takuya Imamura
    • Organizer
      第47回日本発生生物学会年会
    • Place of Presentation
      ウインクあいち(名古屋市)
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Promoter-associated noncoding RNAs mediate gene-specific DNA demethylation for mouse preimplantation development2014

    • Author(s)
      Nobuhiko Hamazaki, Masahiro Uesaka, Kinichi Nakashima, Kiyokazu Agata, Takuya Imamura
    • Organizer
      第8回日本エピジェネティクス研究会年会
    • Place of Presentation
      伊藤国際学術研究センター(東京都文京区)
    • Year and Date
      2014-05-25 – 2014-05-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] PC12細胞分化過程におけるエピジェネティック制御に関わる promoter-associated noncoding RNAの機能2014

    • Author(s)
      山本直樹、上坂将弘、中島欽一、阿形清和、今村拓也
    • Organizer
      第8回日本エピジェネティクス研究会年会
    • Place of Presentation
      伊藤国際学術研究センター(東京都文京区)
    • Year and Date
      2014-05-25 – 2014-05-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] ノンコーディングRNA群による遺伝子活性化と大脳皮質における多様化2014

    • Author(s)
      上坂将弘、西村理、郷康広、中島欽一、阿形清和、今村拓也
    • Organizer
      第七回神経発生討論会
    • Place of Presentation
      大阪大学(吹田市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Epigenome formation in the mammalian brain mediated by promoter-associated noncoding RNA2013

    • Author(s)
      Takuya Imamura
    • Organizer
      Neuro2013 シンポジウム
    • Place of Presentation
      国立京都国際会館(京都市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 霊長類特異的偽遺伝子挿入に由来する非コードRNAによる遺伝子プロモーターのDNA脱メチル化2013

    • Author(s)
      上坂将弘、西村理、郷康広、中島欽一、阿形清和、今村拓也
    • Organizer
      第三回NGS現場の会
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] PC12細胞を用いた不可逆的神経細胞分化制御に関わるRNAの発現動態解析2013

    • Author(s)
      山本直樹、今村拓也
    • Organizer
      第三回NGS現場の会
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] マウス初期発生胚においてプロモーターノンコーディングRNAは卵活性化刺激に応答してCGと非CG配列の脱メチル化を仲介する2012

    • Author(s)
      浜崎伸彦、今村拓也
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2012-12-14
    • Related Report
      2012 Annual Research Report
  • [Presentation] マカクザル特異的偽遺伝子挿入に由来する非コードRNAによる遺伝子プロモーターのDNA脱メチル化2012

    • Author(s)
      上坂将弘、西村理、大石高生、今井啓雄、阿形清和、今村拓也
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2012-12-12
    • Related Report
      2012 Annual Research Report
  • [Presentation] Promoter-associated noncoding RNAs for the sequence-specific epigenetic alterations during mammalian development2012

    • Author(s)
      Takuya Imamura
    • Organizer
      The 1st Hungarian Epigenetics Meeting
    • Place of Presentation
      Semmelweis Egyetem, NET (Budapest, Hungary)(招待講演)
    • Year and Date
      2012-09-20
    • Related Report
      2012 Annual Research Report
  • [Presentation] 遺伝子プロモーター非コードRNAはマウス人為活性化胚のCG/非CG配列の脱メチル化を仲介することで異常DNAメチル化パターン形成に関与する2012

    • Author(s)
      浜崎伸彦、今村拓也
    • Organizer
      第105回日本繁殖生物学会大会
    • Place of Presentation
      筑波大学(つくば市)
    • Year and Date
      2012-09-06
    • Related Report
      2012 Annual Research Report
  • [Presentation] Promoter-associated noncoding RNAs mediate oocyte-activation-induced CpG and non-CpG demethylation in the early mouse embryo2012

    • Author(s)
      Nobuhiko Hamazaki, Takuya Imamura
    • Organizer
      第45回日本発生生物学会年会
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Year and Date
      2012-05-29
    • Related Report
      2012 Annual Research Report
  • [Presentation] マウス初期胚における卵活性化刺激依存性非コードRNA発現による遺伝子プロモーターの脱メチル化2012

    • Author(s)
      浜崎伸彦、今村拓也
    • Organizer
      第6回日本エピジェネティクス研究会年会
    • Place of Presentation
      学術総合センター(東京都千代田区)
    • Year and Date
      2012-05-15
    • Related Report
      2012 Annual Research Report
  • [Presentation] マカクザル特異的偽遺伝子挿入に由来する非コードRNAによる遺伝子プロモーターのDNA脱メチル化2012

    • Author(s)
      上坂将弘、西村理、宇野健一郎、上田泰己、大石高生、今井啓雄、阿形清和、今村拓也
    • Organizer
      第6回日本エピジェネティクス研究会年会
    • Place of Presentation
      学術総合センター(東京都千代田区)
    • Year and Date
      2012-05-15
    • Related Report
      2012 Annual Research Report
  • [Book] Roles of Epigenetics in the Neural Stem Cell and Neuron: In: J. Peedicayil, D. Grayson, D. Avramopoulos (eds), ISBN 9780124171145 Epigenetics in Psychiatry2014

    • Author(s)
      Naoki Yamamoto, Masahiro Uesaka, Takuya Imamura, Kinichi Nakashima
    • Total Pages
      664
    • Publisher
      Elsevier
    • Related Report
      2014 Annual Research Report
  • [Book] Roles of Epigenetics in the Neural Stem Cell and Neuron (J. Peedicayil, D. Avramopoulos, D.R. Grayson編:Epigenetics in Psychiatry 第4章)2014

    • Author(s)
      Naoki Yamamoto, Masahiro Uesaka, Takuya Imamura, Kinichi Nakashima
    • Total Pages
      2000
    • Publisher
      Elsevier
    • Related Report
      2013 Annual Research Report
  • [Book] 「生き物たちのつづれ織り」阿形清和,森哲(監修)下巻2012

    • Author(s)
      今村拓也
    • Total Pages
      234
    • Publisher
      京都大学学術出版会(分担執筆)
    • Related Report
      2012 Annual Research Report
  • [Remarks] 九大プレスリリース

    • URL

      http://www.kyushu-u.ac.jp/pressrelease/2015/2015_02_05.pdf

    • Related Report
      2014 Annual Research Report
  • [Remarks] 九大医学研究院HP

    • URL

      http://www.med.kyushu-u.ac.jp/app/modules/information/detail.php?i=753&c=10

    • Related Report
      2014 Annual Research Report
  • [Remarks] 九大医学系学府HP

    • URL

      http://www.grad.med.kyushu-u.ac.jp/researchinfo/detail.php?i=753

    • Related Report
      2014 Annual Research Report
  • [Remarks] 九州大学医学研究院基盤幹細胞学分野

    • URL

      http://www.scb.med.kyushu-u.ac.jp/imamura

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi