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Generation of transgenic mice expressing equine herpesvirus-1 receptor and its application for antiviral strategies

Research Project

Project/Area Number 24380162
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Applied veterinary science
Research InstitutionHokkaido University

Principal Investigator

KIMURA TAKASHI  北海道大学, (連合)獣医学研究科, 教授 (90261338)

Co-Investigator(Kenkyū-buntansha) SASAKI Nobuya  北里大学, 獣医学部, 教授 (20302614)
Ohnishi Naomi  北海道大学, 人獣共通感染症リサーチセンター, 助教 (50507217)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Keywordsヘルペスウイルス / ウマ / レセプター / トランスジェニックマウス
Outline of Final Research Achievements

Equine major histocompatibility complex (MHC) class I act as receptor molecules that play an important role in EHV-1 entry into equine cells. In this study, transgenic (Tg) mice expressing equine MHC class I were generated to explore whether the ubiquitous expression of equine MHC class I rendered mice more susceptible to EHV-1 infection and then developed more severe disease conditions. Tg mice were inoculated intranasally with EHV-1, euthanized, and subjected to pathological analyses. Tg mice developed more severe bronchointerstitial pneumonia than wild-type (WT) littermate mice. The number of virus antigen-positive cells distributed within lung tissues was higher in Tg mice than that in WT mice. These results suggest that exogenous expression of equine MHC class I may increase susceptibility of lung tissues to EHV-1 infection in Tg mouse. Tg mice having equine MHC class I expression in T cells, which are one of the major target of EHV-1, are also generated.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (24 results)

All 2015 2014 2013 2012 Other

All Journal Article (19 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 19 results) Presentation (5 results)

  • [Journal Article] Detection of novel polyomaviruses in fruit bats in Indonesia.2015

    • Author(s)
      Kobayashi S. et al.
    • Journal Title

      Archives of Virology

      Volume: 160 Issue: 4 Pages: 1075-1082

    • DOI

      10.1007/s00705-015-2349-7

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Detection of coronavirus genomes in Moluccan naked-backed fruit bats in Indonesia.2015

    • Author(s)
      Anindita P. D. et al.
    • Journal Title

      Archives of Virology

      Volume: 160 Issue: 4 Pages: 1113-1118

    • DOI

      10.1007/s00705-015-2342-1

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Full genome sequences of zebra-borne equine herpesvirus type 1 isolated from zebra, onager and Thomson's gazelle.2014

    • Author(s)
      Guo X. et al.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 76 Pages: 1309-1312

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of a novel alphaherpesvirus in fruit bats.2014

    • Author(s)
      Sasaki M. et al.
    • Journal Title

      Journal of Virology

      Volume: 88 Issue: 17 Pages: 9819-9829

    • DOI

      10.1128/jvi.01277-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of the C-terminal region of vervet monkey polyomavirus 1 VP1 in virion formation.2014

    • Author(s)
      Yamaguchi H. et al.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 76 Pages: 637-644

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular epidemiology of paramyxoviruses in frugivorous Eidolon helvum bats in Zambia.2014

    • Author(s)
      Muleya W. et al.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 76 Pages: 611-614

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular epidemiology of paramyxoviruses in Zambian wild rodents and shrews.2014

    • Author(s)
      Sasaki M. et al.
    • Journal Title

      Journal of General Virology

      Volume: 95 Issue: 2 Pages: 325-330

    • DOI

      10.1099/vir.0.058404-0

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of tracking system for West Nile virus entry and evidence of microtubule involvement in particle transport2013

    • Author(s)
      Makino Y, Suzuki T, Hasebe R, Kimura T, Maeda A, Takahashi H, Sawa H
    • Journal Title

      J Virol Methods

      Volume: 195 Pages: 250-257

    • DOI

      10.1016/j.jviromet.2013.10.002

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cysteine Residues in the Major Capsid Protein, Vp1, of the JC Virus Are Important for Protein Stability and Oligomer Formation2013

    • Author(s)
      Kobayashi S, Suzuki T, Igarashi M, Orba Y, Ohtake N, Nagakawa K, Niikura K, Kimura T, Kasamatsu H, Sawa H
    • Journal Title

      PLoS One

      Volume: 8(10) Issue: 10 Pages: e76668-e76668

    • DOI

      10.1371/journal.pone.0076668

    • NAID

      120005350093

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Human parainfluenza virus type 3 in wild primates, Zambia.2013

    • Author(s)
      Sasaki M. et al.
    • Journal Title

      Emerging Infectious Diseases

      Volume: 19 Issue: 9 Pages: 1500-1503

    • DOI

      10.3201/eid1909.121404

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of Japanese encephalitis virus infection in an immortalized mesencephalic cell line, CSM14.1.2013

    • Author(s)
      Kimura T. et al.
    • Journal Title

      Microbiology and Immunology

      Volume: 57 Issue: 10 Pages: 723-731

    • DOI

      10.1111/1348-0421.12085

    • NAID

      40019842450

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heterosubtypic antiviral activity of hemagglutinin-specific antibodies induced by intranasal immunization with inactivated influenza viruses in mice.2013

    • Author(s)
      Muramatsu M. et al.
    • Journal Title

      PLoS One

      Volume: 8 Issue: 8 Pages: e71534-e71534

    • DOI

      10.1371/journal.pone.0071534

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a novel polyomavirus from vervet monkeys in Zambia.2013

    • Author(s)
      Yamaguchi H, Kobayashi S, Ishii A, Ogawa H, Nakamura I, Moonga L, Hang'ombe BM, Mweene AS, Thomas Y, Kimura T, Sawa H, Orba Y.
    • Journal Title

      J Gen Virol.

      Volume: 94(Pt 6) Issue: 6 Pages: 1357-64

    • DOI

      10.1099/vir.0.050740-0

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Population genetic analysis and sub-structuring of Theileria parva in the northern and eastern parts of Zambia.2012

    • Author(s)
      Muleya W, et al.
    • Journal Title

      Parasites and Vectors

      Volume: 5 Issue: 1 Pages: 255-255

    • DOI

      10.1186/1756-3305-5-255

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular detection of a novel paramyxovirus in fruit bats from Indonesia.2012

    • Author(s)
      Sasaki M, et al.
    • Journal Title

      Virology Journal

      Volume: 9 Issue: 1 Pages: 240-240

    • DOI

      10.1186/1743-422x-9-240

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of JC virus agnoprotein in virion formation.2012

    • Author(s)
      Suzuki T, Semba S, Sunden Y, Orba Y, Kobayashi S, Nagashima K, Kimura T, Hasegawa H, Sawa H.
    • Journal Title

      Microbiol Immunol

      Volume: 56(9) Issue: 9 Pages: 639-46

    • DOI

      10.1111/j.1348-0421.2012.00484.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pathological Examination of Lung Tissues in Influenza A Virus-Infected Mice2012

    • Author(s)
      Muto NA, Sunden Y, Hattori T, Fujikura D, Nakayama Y, Miyazaki T, Maruyama M, Kimura T, Sawa H.
    • Journal Title

      Japanese Journal of Infectious Diseases

      Volume: 65 Issue: 5 Pages: 383-391

    • DOI

      10.7883/yoken.65.383

    • NAID

      130001931846

    • ISSN
      1344-6304, 1884-2836
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Accumulation of ubiquitinated proteins is related to the West Nile virus-induced neuronal apoptosis2012

    • Author(s)
      Kobayashi S, Orba Y, Yamaguchi H, Kimura T, Sawa H
    • Journal Title

      Neuropathology

      Volume: 32(4) Issue: 4 Pages: 398-405

    • DOI

      10.1111/j.1440-1789.2011.01275.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibitory effects of an M2-specific monoclonal antibody on different strains of influenza A virus.2012

    • Author(s)
      Muto N. A, et al.
    • Journal Title

      Japanese Journal of Veterinary Research

      Volume: 60 Pages: 71-83

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] ウマヘルペスウイルス1型レセプター遺伝子導入マウスの作製2015

    • Author(s)
      港江利奈ら
    • Organizer
      第10回北海道獣医病理三大学セミナー
    • Place of Presentation
      合宿の宿ひまわり(北海道夕張市)
    • Year and Date
      2015-08-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] ウイルス感染と免疫.日本獣医病理学会スライドセミナー"炎症と免疫"2013

    • Author(s)
      木村 享史
    • Organizer
      第155回日本獣医学会学術集会
    • Place of Presentation
      東京大学(東京都)(招待講演)
    • Year and Date
      2013-03-30
    • Related Report
      2012 Annual Research Report
  • [Presentation] ラット中脳由来神経細胞株CSM14.1における日本脳炎ウイルス感染様式

    • Author(s)
      木村 享史ら
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜大学(岐阜県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] オートファジーによるウエストナイルウイルス増殖抑制機構の解明

    • Author(s)
      小林 進太郎ら
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜大学(岐阜県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] インドネシア共和国に生息するオオコウモリから分離した新規ヘルペスウイルスの性状解析

    • Author(s)
      佐々木 道仁ら
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜大学(岐阜県)
    • Related Report
      2013 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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