Generation of transgenic mice expressing equine herpesvirus-1 receptor and its application for antiviral strategies
| Project/Area Number |
24380162
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Hokkaido University |
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Principal Investigator |
KIMURA TAKASHI 北海道大学, (連合)獣医学研究科, 教授 (90261338)
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Nobuya 北里大学, 獣医学部, 教授 (20302614)
Ohnishi Naomi 北海道大学, 人獣共通感染症リサーチセンター, 助教 (50507217)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Keywords | ヘルペスウイルス / ウマ / レセプター / トランスジェニックマウス |
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| Outline of Final Research Achievements |
Equine major histocompatibility complex (MHC) class I act as receptor molecules that play an important role in EHV-1 entry into equine cells. In this study, transgenic (Tg) mice expressing equine MHC class I were generated to explore whether the ubiquitous expression of equine MHC class I rendered mice more susceptible to EHV-1 infection and then developed more severe disease conditions. Tg mice were inoculated intranasally with EHV-1, euthanized, and subjected to pathological analyses. Tg mice developed more severe bronchointerstitial pneumonia than wild-type (WT) littermate mice. The number of virus antigen-positive cells distributed within lung tissues was higher in Tg mice than that in WT mice. These results suggest that exogenous expression of equine MHC class I may increase susceptibility of lung tissues to EHV-1 infection in Tg mouse. Tg mice having equine MHC class I expression in T cells, which are one of the major target of EHV-1, are also generated.
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Report
(5 results)
Research Products
(24 results)
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[Journal Article] Cysteine Residues in the Major Capsid Protein, Vp1, of the JC Virus Are Important for Protein Stability and Oligomer Formation2013
Author(s)
Kobayashi S, Suzuki T, Igarashi M, Orba Y, Ohtake N, Nagakawa K, Niikura K, Kimura T, Kasamatsu H, Sawa H
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Journal Title
PLoS One
Volume: 8(10)
Issue: 10
Pages: e76668-e76668
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Identification of a novel polyomavirus from vervet monkeys in Zambia.2013
Author(s)
Yamaguchi H, Kobayashi S, Ishii A, Ogawa H, Nakamura I, Moonga L, Hang'ombe BM, Mweene AS, Thomas Y, Kimura T, Sawa H, Orba Y.
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Journal Title
J Gen Virol.
Volume: 94(Pt 6)
Issue: 6
Pages: 1357-64
DOI
Related Report
Peer Reviewed
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