Study on the edible vaccine for chickens using recombinant barley with beta-glucanase

• Project/Area Number: 24380164
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: The University of Tokyo
• Principal Investigator: Matsumoto Yasunobu 東京大学, 農学生命科学研究科, 准教授 (90251420)
• Co-Investigator(Kenkyū-buntansha): YAMAKAWA TAKASHI 東京大学, 大学院農学生命科学研究科, 教授 (20134520)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000); Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
• Keywords: 家禽 / 食べるワクチン / ベータグルカナーゼ / ニューカッスル病ウイルス / オオムギ

Outline of Final Research Achievements

The production of barley carrying F or HN genes of Newcastle disease virus (NDV) to use as edible vaccine was planned. Antigenicity of F protein as well as experimental NDV infection system of chicken was established. Establishment of NDV F and HN producing barley could not be obtained, however, virological evidences such as the recognition of NDV virion by antibody raised to N-terminal fragment of F protein and neutralizing antibody inducing potential by HR1-immunization have been obtained.