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Transactivation potencies of peroxisome proliferator-activated receptor alpha by brominated flame retardants

Research Project

Project/Area Number 24380179
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Boundary agriculture
Research InstitutionEhime University (2014)
Shokei Junior College (2012-2013)

Principal Investigator

ISHIBASHI HIROSHI  愛媛大学, 農学部, 准教授 (90403857)

Co-Investigator(Kenkyū-buntansha) ARIZONO Koji  熊本県立大学, 環境共生学部, 教授 (70128148)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Keywords残留性有機汚染物質 / 臭素系難燃剤 / 核内受容体 / PPARα / 環境汚染 / 生態系保全 / リスク評価 / 遺伝子 / 生態系 / PPARα
Outline of Final Research Achievements

The present study assessed the transactivation potencies of the Baikal seal (Pusa sibirica) peroxisome proliferator-activated receptor α (bsPPARα) by brominated flame retardants (BFRs) using an in vitro reporter gene assay. Among seven BFRs treated in a range of 2.4-50 microM concentration, three PBDEs, BDE47, 99 and 153, induced bsPPARα-mediated transcriptional activities in a dose-dependent manner. To compare the bsPPARα transactivation potencies by PBDEs, the present study estimated the perfluorooctanoic acid (PFOA) induction equivalency factors (IEFs). The order of IEFs for the PBDEs was as follows; BDE153 (IEF: 17) > BDE99 (12) > BDE47 (7.4) >> PFOA (1.0) >>> BDE100, BDE154, BDE183 and HBCDs (not activated). This is the first study in which the transactivation potencies of PPARα by PBDEs in aquatic mammals were clarified. Our study demonstrates that the PPARα-mediated response may be a useful biomarker to evaluate potential biological effects of PBDEs in wildlife.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (34 results)

All 2015 2014 2013 2012

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 4 results) Presentation (23 results) Book (3 results)

  • [Journal Article] Trophic magnification of polychlorinated biphenyls and polybrominated diphenyl ethers in an estuarine food web of the Ariake Sea, Japan2015

    • Author(s)
      Kobayashi, J., Imuta, Y., Komorita, T., Yamada, K., Ishibashi, H., Ishihara, F., Nakashima, N., Sakai, J., Arizono, K., Koga, M.
    • Journal Title

      Chemosphere

      Volume: 118 Pages: 201-206

    • DOI

      10.1016/j.chemosphere.2014.08.066

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification and characterization of the androgen receptor from the American alligator, Alligator mississippiensis2015

    • Author(s)
      Miyagawa, S., Yatsu, R., Kohno, S., Doheny, B.M., Ogino, Y., Ishibashi, H., Katsu, Y., Ohta, Y., Guillette, L.J.Jr., Iguchi, T.
    • Journal Title

      Endocrinology

      Volume: in press

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Endocrine-disrupting potentials of equine estrogens equilin, equilenin, and their metabolites, in the medaka Oryzias latipes: in silico and DNA microarray studies2015

    • Author(s)
      Uchida, M., Ishibashi, H., Yamamoto, R., Koyanagi, A., Kusano, T., Tominaga, N., Ishibashi, Y., Arizono, K.
    • Journal Title

      Journal of Applied Toxicology

      Volume: in press Issue: 9 Pages: 1040-1048

    • DOI

      10.1002/jat.3098

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of lithium on growth, maturation, reproduction, and gene expression in the nematode Caenorhabditis elegans2015

    • Author(s)
      Inokuchi, A., Yamamoto, R., Morita, F., Takumi, S., Matsusaki, H., Ishibashi, H., Tominaga, T., Arizono, K.
    • Journal Title

      Journal of Applied Toxicology

      Volume: in press Issue: 9 Pages: 999-1006

    • DOI

      10.1002/jat.3058

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparative study of the biological effects of antimicrobials, triclosan and trichlocarban, for C. elegans2014

    • Author(s)
      Inokuchi, A., Nihira, M., Minakoshi, M., Yamamoto, R., Ishibashi, H., Tominaga, N., Arizono, K.
    • Journal Title

      Journal of Environment and Safety

      Volume: 4

    • NAID

      130004566839

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of lithium on growth, maturation, reproduction, and gene expression in the nematode Caenorhabditis elegans2014

    • Author(s)
      Inokuchi, A., Yamamoto, R., Morita, F., Takumi, S., Matsusaki, H., Ishibashi, H., Tominaga, T., Arizono, K.
    • Journal Title

      Journal of Applied Toxicology

      Volume: 34

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative analysis of the interaction of constitutive androstane receptor with chemicals and steroid receptor coactivator 1 using surface Plasmon resonance biosensor systems : A case study of the Baikal seal (Pusa sibirica) and mouse.2013

    • Author(s)
      Dau, P.T., Sakai, H., Hirano. M., Ishibashi, H., Tanaka, Y., Kameda, K., Fujino, T., Kim, E,Y. and Iwata, H.
    • Journal Title

      Toxicol. Sci.

      Volume: 131(1) Issue: 1 Pages: 116-127

    • DOI

      10.1093/toxsci/kfs288

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Study of dietary phytoestrogens and estrogenic activity in pet animal diets2013

    • Author(s)
      Nakashima, S., Ji, H., Ishitobi, S., Kawazoe, S., Koga, M., Shinohara, R., Ishibashi, H., Tominaga, N., Arizono, K.
    • Journal Title

      Japanese Journal of Food Chemistry and Safety

      Volume: 20 Pages: 22-30

    • NAID

      110009603493

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] メダカを用いたエクインエストロゲン類のエストロゲン様作用の評価2015

    • Author(s)
      有薗幸司、石橋弘志、内田雅也、宮川信一、冨永伸明、石橋康弘、井口泰泉
    • Organizer
      第42回日本毒性学会学術年会
    • Place of Presentation
      石川県立音楽堂、金沢市アートホール、ホテル日航金沢
    • Year and Date
      2015-06-29 – 2015-07-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] In silico, in vitroおよびin vivo試験系を用いた医薬品プレマリン成分の女性ホルモン様作用の評価2015

    • Author(s)
      有薗幸司、石橋弘志、内田雅也、宮川信一、一川暢宏、冨永伸明、石橋康弘、井口泰泉
    • Organizer
      第24回環境化学討論会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2015-06-24 – 2015-06-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Gain of function mutations in two paralogous Androgen receptor (AR) genes of teleosts: Implications for evolution of AR gene function2015

    • Author(s)
      Ogino, Y., Kuraku, S., Ishibashi, H., Miyakawa, H., Miyagawa, S., Yamada, G., Baker, M.E., Iguchi, T.
    • Organizer
      48th Annual Meeting of the Japanese Society of Developmental Biologists
    • Place of Presentation
      Tsukuba
    • Year and Date
      2015-06-02 – 2015-06-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] In vivo and in silico studies to evaluate the estrogenic potential of bisphenol analogs in medaka, Oryzias latipes2014

    • Author(s)
      Yamaguchi, A., Ishibashi, H., Arizono, K., Tominaga, N.
    • Organizer
      環境ホルモン学会第17回研究発表会
    • Place of Presentation
      東京大学山上会館
    • Year and Date
      2014-12-09 – 2014-12-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] 医薬品プレマリン成分のメダカに対するエストロゲン様作用2014

    • Author(s)
      中尾彩美、石橋弘志、内田雅也、山元涼子、小柳暁子、草野輝彦、冨永伸明、石橋康弘、有薗幸司
    • Organizer
      環境ホルモン学会第17回研究発表会
    • Place of Presentation
      東京大学山上会館
    • Year and Date
      2014-12-09 – 2014-12-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] In vivo and in silico studies to evaluate the estrogenic effects of bisphenol analogues on medaka2014

    • Author(s)
      Arizono, K., Ishibashi, H., Tominaga, N.
    • Organizer
      Society of Environmental Toxicology and Chemistry North America 35th Annual Meeting
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      2014-11-09 – 2014-11-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 河口域におけるポリ塩化ビフェニル(PCBs)の動態2014

    • Author(s)
      小森田智大、小林 淳、伊牟田 優希、石原史隆、中島尚哉、石橋弘志、堤 裕昭
    • Organizer
      日本海洋学会秋季大会
    • Place of Presentation
      長崎大学文教キャンパス
    • Year and Date
      2014-09-13 – 2014-09-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] メダカ(Oryzias latipes)に対するエクインエストロゲンの潜在的内分泌かく乱作用2014

    • Author(s)
      内田雅也、小柳暁子、草野輝彦、石橋弘志、冨永伸明、石橋康弘、有薗幸司
    • Organizer
      第20回日本環境毒性学会研究発表会
    • Place of Presentation
      富山国際会議場
    • Year and Date
      2014-09-10 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 有明海の河口域におけるポリ塩化ビフェニルの食物網蓄積モデルの構築と検証2014

    • Author(s)
      小林淳、小森田智大、石橋弘志、有薗幸司、古賀実.有明海の河口域におけるポリ塩化ビフェニルの食物網蓄積モデルの構築と検証
    • Organizer
      第23回環境化学討論会
    • Place of Presentation
      京都大学百周年記念時計台記念館、芝蘭会館、芝蘭会館別館
    • Year and Date
      2014-05-14 – 2014-05-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] 汽水域に棲息する多毛類のポリ塩化ビフェニルの取込み経路と体内動力学2014

    • Author(s)
      川中理可、小林淳、石原史隆、櫻井健郎、小森田智大、石橋弘志、有薗幸司、古賀実
    • Organizer
      第23回環境化学討論会
    • Place of Presentation
      京都大学百周年記念時計台記念館、芝蘭会館、芝蘭会館別館
    • Year and Date
      2014-05-14 – 2014-05-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] 魚類におけるポリ臭素化ジフェニルエーテルの体内分配に及ぼす代謝の影響2014

    • Author(s)
      石原史隆、小林淳、小森田智大、石橋弘志、有薗幸司、古賀実
    • Organizer
      第23回環境化学討論会
    • Place of Presentation
      京都大学百周年記念時計台記念館、芝蘭会館、芝蘭会館別館
    • Year and Date
      2014-05-14 – 2014-05-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] 大牟田川河口域における残留性有機汚染物質の食物連鎖蓄積について2014

    • Author(s)
      伊牟田優希、小林淳、小森田智大、山田勝雅、石橋弘志、有薗幸司、古賀
    • Organizer
      第23回環境化学討論会
    • Place of Presentation
      京都大学百周年記念時計台記念館、芝蘭会館、芝蘭会館別館
    • Year and Date
      2014-05-14 – 2014-05-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] 有明海河口域における有機フッ素化合物の生物蓄積2014

    • Author(s)
      前田佳貴、伊牟田優希、石原史隆、中島尚哉、小林淳、小森田智大、石橋弘志、有薗幸司、古賀実
    • Organizer
      第23回環境化学討論会
    • Place of Presentation
      京都大学百周年記念時計台記念館、芝蘭会館、芝蘭会館別館
    • Year and Date
      2014-05-14 – 2014-05-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Transcriptomic analysis of the liver of Japanese medaka (Oryzias latipes) exposed to Kanechlor-500, BDE47 or PFOS2013

    • Author(s)
      Ishibashi, H., Uchida, M., Hirano, M., Kobayashi, J., Komorita, T., Nakamura, H., Kagami, Y., Kusano, T., Ishibashi, Y., Arizono, K.
    • Organizer
      7th International Conference on Marine Pollution and Ecotoxicology
    • Place of Presentation
      Hong Kong
    • Related Report
      2013 Annual Research Report
  • [Presentation] 海産魚類における残留性有機汚染物質の体内分布特性2013

    • Author(s)
      石原史隆、小林淳、小森田智大、中島尚哉、伊牟田優希、梅原亮、石橋弘志、有薗幸司、堤裕昭、古賀実
    • Organizer
      第22回環境化学討論会
    • Place of Presentation
      東京都府中市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 有明海の河口域における残留性有機汚染物質の空間分布と分配2013

    • Author(s)
      小林淳、小森田智大、中島尚哉、石原史隆、伊牟田優希、梅原亮、石橋弘志、有薗幸司、堤裕昭、古賀実
    • Organizer
      第22回環境化学討論会
    • Place of Presentation
      東京都府中市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 有明海の河口域に棲息する水生生物への残留性有機汚染物質の蓄積解析2013

    • Author(s)
      伊牟田優希、小林淳、小森田智大、中島尚哉、石原史隆、梅原亮、石橋弘志、有薗幸司、堤裕昭、古賀実
    • Organizer
      第22回環境化学討論会
    • Place of Presentation
      東京都府中市
    • Related Report
      2013 Annual Research Report
  • [Presentation] In vitro and in silico analyses to predict the effects of environmental chemicals through the mysid ecdysteroid receptor signaling pathway2013

    • Author(s)
      Hirano, M., Ishibashi, H., Kim, E. Y., Arizono, K., Iwata, H.
    • Organizer
      The 33rd International Symposium on Halogenated Persistent Organic Pollutants - Dioxin 2013
    • Place of Presentation
      Korea
    • Related Report
      2013 Annual Research Report
  • [Presentation] PPCPsのエストロゲン様作用のin silicoスクリーニング2013

    • Author(s)
      瀬原葉子、石橋弘志、石橋康弘、有薗幸司
    • Organizer
      環境ホルモン学会第16回研究発表会
    • Place of Presentation
      東京都文京区
    • Related Report
      2013 Annual Research Report
  • [Presentation] Identification of amino acid residues critical for ligand binding and transactivation in ecdysone receptor2013

    • Author(s)
      Hirano, M., Ishibashi, H., Kim, E. Y., Arizono, K., Iwata, H.
    • Organizer
      環境ホルモン学会第16回研究発表会
    • Place of Presentation
      東京都文京区
    • Related Report
      2013 Annual Research Report
  • [Presentation] Functional analyses of the mysid ecdysteroid receptor : DNA binding ability, ligand-induced transactivation and in silico modeling2012

    • Author(s)
      Hirano, M.
    • Organizer
      Society of Environmental Toxicology and Chemistry Asia Pacific 2012 Meeting
    • Place of Presentation
      熊本
    • Related Report
      2012 Annual Research Report
  • [Presentation] Binding of perfluorochemicals to PPARa : toward establishing the linkage between the initiating event and its downstream signaling2012

    • Author(s)
      Ishibashi, H.
    • Organizer
      Society of Environmental Toxicology and Chemistry North America 33rd Annual Meeting
    • Place of Presentation
      アメリカ
    • Related Report
      2012 Annual Research Report
  • [Presentation] Transactivation potencies of the Baikal seal (Pusa sibirica)peroxisome proliferator-activated receptor a by brominated flame retardants2012

    • Author(s)
      Ishibashi, H.
    • Organizer
      環境ホルモン学会第15回研究発表会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Book] 健康と環境の科学(川添禎浩編)2014

    • Author(s)
      石橋弘志、有薗幸司
    • Total Pages
      161
    • Publisher
      講談社
    • Related Report
      2014 Annual Research Report
  • [Book] 健康・栄養科学シリーズ 食べ物と健康 食品の安全(編集 有薗幸司)2013

    • Author(s)
      石橋弘志、髙本亜希子
    • Total Pages
      249
    • Publisher
      南江堂
    • Related Report
      2013 Annual Research Report
  • [Book] 健康と環境の科学(川添禎浩 編)2013

    • Author(s)
      石橋弘志、有薗幸司
    • Total Pages
      166
    • Publisher
      講談社
    • Related Report
      2013 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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