| Project/Area Number |
24380180
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Boundary agriculture
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| Research Institution | Forestry and Forest Products Research Institute |
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Principal Investigator |
NAKAMURA Masaya 独立行政法人森林総合研究所, きのこ・微生物研究領域, 室長 (50353793)
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| Co-Investigator(Kenkyū-buntansha) |
SHIKINAKA Kazuhiro 東京農工大学, 工学系研究科, 助教 (00507189)
KATAYAMA Yoshihiro 日本大学, 生物資源科学部, 教授 (10214339)
OTSUKA Yuichiro 森林総合研究所, バイオマス化学研究領域, 主任研究員 (80455261)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Keywords | リグニン / プロトカテク酸3,4-ジオキシゲナーゼ / 2-ピロン－4,6-ジカルボン酸 / 3-カルボキシムコノラクトン / バニリン / プロトカテク酸4,5-ジオキシゲナーゼ / 2-ピロン4,6-ジカルボン酸 / アミド化合物 / ポリエステル / カルボキシムコノラクトン / 3-カルボキシムコノラクトンジクロリド化合物 / バニリン酸 / バイオマス |
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| Outline of Final Research Achievements |
We have produce 2-pyrone-4,6-dicarboxylic acid (PDC) and 3-carboxymuconolactone (3CML) as a platform chemicals with genes coding for vanillin dehydrogenase (lig V), vanillic acid demethylase (van AB), protocatechuic acid 4,5-dioxygenase (lig AB) and 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase (lig C) from the lignin degrading soil bacterium, Sphingobium sp. SYK-6, and protocatechuic acid 3,4-dioxygenasd (pca HG) from aromatic compound degrader P.putida KT2440, 3-carboxy-cis,cis-muconate lactonizing enzyme gene (pca B) from Neurpspora crassa respectively.
The PDC polyesters bearing the aromatic groups adjacent to the PDC nuclei exhibited high Td5 (5 wt% loss temperature), good enough to utilize them as thermoplastics. The 3-CML was converted into the corresponding acid chloride derivative, 3-CML dichloride and amid derivative with n-hexyl isocyanate respectively for polymerization.
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