Respiration control and temperature-sensing in thermogenic plants.

• Project/Area Number: 24380182
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Iwate University
• Principal Investigator: Ito Kikukatsu 岩手大学, 農学部, 教授 (50232434)
• Co-Investigator(Kenkyū-buntansha): 長田 洋 岩手大学, 工学部, 教授 (10261463)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000); Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2012: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: 呼吸制御 / 発熱植物 / ザゼンソウ / 温度センシング / 細胞機能

Outline of Final Research Achievements

In the present study, skunk cabbage (Symplocarpus renifolius), was used to uncover the mechanism of respiration control and temperature-sensing mechanisms during homeothermic heat-production. Our data showed that mitochondria from thermogenic spadices possess a specific temperature-dependent respiration, and that thermodynamic analyses could be a useful tool to determine the overall activation energy of respiration control under different temperatures.

Research Products

• [Int'l Joint Research] アデレード大学(オーストラリア)
• [Int'l Joint Research] サセックス大学(英国)
• [Journal Article] The biochemical basis for thermoregulation in heat-producing flowers. (2016)
  • Author(s): Y. Umekawa, R.S. Seymour & K. Ito
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 24830-24830
  • DOI: 10.1038/srep24830
  • NAID: 120007179837
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The oxygen supply to thermogenic flowers (2015)
  • Author(s): Roger S. Seymour, Kikukatsu Ito, Yui Umekawa, Philip D.G. Matthews & Stergios Arg. Pirintsos
  • Journal Title: Plant, Cell & Environment Volume: 38 Issue: 4 Pages: 827-837
  • DOI: 10.1111/pce.12454
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Transcriptome analysis of thermogenic Arum concinnatum reveals the molecular components of floral scent production (2015)
  • Author(s): Yoshihiko Onda, Keiichi Mochida, Takuhiro Yoshida, Tetsuya Sakurai, Roger Seymour, Yui Umekawa, Stergios Arg. Pirintsos, Kazuo Shinozaki, Kikukatsu Ito
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 1-12
  • DOI: 10.1038/srep08753
  • NAID: 120007179835
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] ミトコンドリアにおけるシアン耐性呼吸酵素（AOX）の構造と機能 (2014)
  • Author(s): 伊藤菊一
  • Journal Title: 光合成研究 Volume: 24 Pages: 10-17
  • Peer Reviewed / Open Access
• [Journal Article] Metabolite profiling reveals tissue- and temperature-specific metabolomic responses in thermoregulatory male florets of Dracunculus vulgaris (Araceae) (2013)
  • Author(s): Ito, K., Takahashi, H., Umekawa, Y., Imamura, T., Kawasaki, S., Ogata, T., Kakizaki, Y. and Seymour, R.S.
  • Journal Title: Metabolomics Volume: (in press) Issue: 4 Pages: 919-930
  • DOI: 10.1007/s11306-013-0509-x
  • Peer Reviewed
• [Journal Article] Unraveling the Heater: New Insights into the Structure of the Alternative Oxidase (2013)
  • Author(s): Anthony L. Moore, Tomoo Shiba, Luke Young, Shigeharu Harada, Kiyoshi Kita, Kikukatsu Ito
  • Journal Title: Annual Review of Plant Biology Volume: 64 Issue: 1 Pages: 637-663
  • DOI: 10.1146/annurev-arplant-042811-105432
  • Peer Reviewed
• [Journal Article] Different molecular bases underlie the mitochondrial respiratory activity in the homoeothermic spadices of Symplocarpus renifolius and transiently-thermogenic appendices of Arum maculatum (2012)
  • Author(s): Yusuke Kakizaki
  • Journal Title: Biochemical Journal Volume: 445 Issue: 2 Pages: 237-246
  • DOI: 10.1042/bj20111978
  • Peer Reviewed
• [Presentation] ザゼンソウの発熱制御安定性に関する研究 (2015)
  • Author(s): 高橋暁生，田中大貴，舘松達矢，千葉茂樹，伊藤菊一，長田 洋
  • Organizer: 第70回応用物理学会東北支部学術講演会
  • Place of Presentation: 平川市
  • Year and Date: 2015-12-03
• [Presentation] ザゼンソウにおける温度変化と逆相関を示す呼吸調節メカニズム (2015)
  • Author(s): 梅川 結，伊藤菊一
  • Organizer: 第38回分子生物学会年会・第88回生化学会大会合同大会 BMB2015
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] 植物ザゼンソウの恒温性メカニズムにおける負の活性化エネルギー (2015)
  • Author(s): 梅川 結，伊藤菊一
  • Organizer: 平成27年度 日本農芸化学会 東北支部 第150回大会
  • Place of Presentation: 仙台
  • Year and Date: 2015-10-03
• [Presentation] de novo Transcriptome Analysis Reveals the Molecular Components of Floral Scent Production in Thermogenic Arum concinnatum (2015)
  • Author(s): Yoshihiko Onda, Keiichi Mochida, Takuhiro Yoshida, Tetsuya Sakurai, Roger Seymour, Yui Umekawa, Stergios Arg. Pirintsos, Kazuo Shinozaki, Kikukatsu Ito
  • Organizer: International Plant & Animal Genome XXIII
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2015-01-10 – 2015-01-14
• [Presentation] ザゼンソウの温度制御における地下器官の機能に関する研究 (2014)
  • Author(s): 工藤俊祐、高橋暁生、千葉茂樹、伊藤孝徳、伊藤菊一、長田 洋
  • Organizer: 第66回応用物理学会東北支部学術講演会
  • Place of Presentation: 仙台
  • Year and Date: 2014-12-04 – 2014-12-05
• [Presentation] ザゼンソウ発熱組織から調製したミトコンドリアにおけるNADH呼吸の温度応答性に関する解析 (2014)
  • Author(s): 梅川 結、Sayed Md Abu, 清藤駿成、 伊藤菊一
  • Organizer: 第87回日本生化学会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] Rotenone-insensitive internal alternative NADPH dehydrogenase-dependent respiration in the mitochondria from skunk cabbage: temperature responses of COX- and AOX-mediated respiration pathways. (2014)
  • Author(s): Yui Umekawa, Takanari Seito, Md Abu Sayed , and Kikukatsu Ito
  • Organizer: EBEC2014
  • Place of Presentation: Lisbone
  • Year and Date: 2014-07-12 – 2014-07-17
• [Presentation] Symplocarpus renifoliusにおける発熱調節と代謝変動に関する網羅的解析 (2012)
  • Author(s): 梅川結,高橋秀行,今村智宏,伊藤菊一
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡(福岡)
  • Year and Date: 2012-12-15
• [Presentation] ミトコンドリアにおけるシアン耐性呼吸酵素（AOX）の構造と機能
  • Author(s): 伊藤菊一
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 北海道大学（札幌）
  • Invited
• [Remarks] ザゼンソウの恒温性メカニズムを解明
  • URL: http://www.iwate-u.ac.jp/news/news201604.shtml#2766