Molecular mechanism of ascorbate biosynthesis and its pool size in plant source and sink tissues
Project/Area Number |
24380186
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Applied molecular and cellular biology
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Research Institution | Shimane University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
MARUTA Takanori 島根大学, 生物資源科学部, 准教授 (50607439)
|
Research Collaborator |
TANEDA Hayato
SODEYAMA Tsubasa
Smirnoff Nicholas
|
Project Period (FY) |
2012-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
|
Keywords | アスコルビン酸生合成 / 光合成生物 / 光調節 / アスコルビン酸 / リン酸化/脱リン酸化 / 発現遺伝子解析 / ガラクツロン酸経路 / マンノース/ガラクトース経路 / 生合成 / シロイヌナズナ / 相互作用タンパク質 / モデル植物 / 生合成調節 / ソース・シンク器官 / タンパク質相互作用 / 遺伝子発現調節 |
Outline of Final Research Achievements |
The aim of this study is to clarify regulation mechanism of plant vitamin C biosynthesis in response to light. We have done molecular physiological analyses using Arbidopsis, tomato, and moss plants, and concluded that photosynthesis electron transport system is working as a trigger for light dependent vitamin C synthesis. In addition, we obtained some experimental evidences that the key regulatory factors contribution downstream of this trigger is VTC2 and VTC3, encoding GDP-L-galactose phosphorylase and a novel protein kinase/phosphatase, respectively.
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Report
(5 results)
Research Products
(41 results)
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[Journal Article] Transcriptional control of vitamin C defective 2 and tocopherol cyclase genes by light and plastid-derived signals: The partial involvement of GENOMES UNCOUPLED 1.2015
Author(s)
Tanaka, H., Maruta. T., Tamoi, M., Yabuta, Y., Yoshimura, K., Ishikawa, T., Shigeoka S.
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Journal Title
Plant Science
Volume: 231
Pages: 20-29
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Transient expression analysis revealed the importance of VTC2 expression level in light/dark regulation of ascorbate biosynthesis in Arabidopsis.2014
Author(s)
Yoshimura, K., Nakane, T., Kume S., Shiomi, Y., Maruta, T., Ishikawa, T. and Shigeoka, S.
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Journal Title
Biosci. Biotechnol. Biochem.
Volume: 78
Pages: 60-66
Related Report
Peer Reviewed
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[Journal Article] Activation of γ-aminobutyrate production by chloroplastic H2O2 is associated with the oxidative stress response.2013
Author(s)
Maruta, T., Ojiri, M., Noshi, M., Tamoi, M., Ishikawa, T. and Shigeoka, S.
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Journal Title
Biosci. Biotechnol. Biochem.
Volume: 77
Pages: 422-425
Related Report
Peer Reviewed
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[Journal Article] H2O2-triggered retrograde signaling from chloroplasts to nucleus plays a specific role in the response to stress2012
Author(s)
Maruta, T., Noshi, M., Tanouchi, A., Tamoi, M., Yabuta, Y., Yoshimura, K.,Ishikawa, T., and Shigeoka, S.
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Journal Title
J. Biol. Chem
Volume: 287
Issue: 15
Pages: 11717-11729
DOI
Related Report
Peer Reviewed
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