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Development of imaging probe to detect lipid derived radicals from whole body to cells

Research Project

Project/Area Number 24390011
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Physical pharmacy
Research InstitutionKyushu University

Principal Investigator

YAMADA Kenichi  九州大学, 薬学研究科(研究院), 准教授 (60346806)

Co-Investigator(Kenkyū-buntansha) KOBORI Yasuhiro  神戸大学, 大学院理学研究科, 教授 (00282038)
Co-Investigator(Renkei-kenkyūsha) AOKI Ichio  独立行政法人放射線医学総合研究所, 分子イメージング研究センター, チームリーダー (10319519)
MIURA Daisuke  九州大学, 先端融合医療レドックスナビ研究拠点, 准教授 (40532627)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Keywords薬学 / 生体分子 / 脂質 / ストレス / 脂質ラジカル / 蛍光 / 酸化ストレス / ニトロキシド / 脂質過酸化 / 活性酸素 / ESR / MRI
Outline of Final Research Achievements

Lipid derived radicals and its metabolic products are closely involved in the pathogenesis of oxidative stress diseases such as inflammation and angiogenesis through the formation of protein or DNA complex. The starting point of lipid peroxide generation is lipid derived radicals which increase explosively via radical chain reaction. Therefore, the trapping of lipid derived radicals is useful to understanding the mechanism of formation of oxidative stress diseases, and to suppress the following chain reaction. On the other hands, nitroxides having stable unpaired electron allows spin trapping with carbon-centered radicals. Hence, in this study, we aimed to develop and evaluate lipid derived detection method using profluorescent nitroxide switching methods.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (18 results)

All 2015 2014 2013 2012

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Acknowledgement Compliant: 1 results) Presentation (12 results) (of which Invited: 2 results)

  • [Journal Article] Brain imaging in methamphetamine-treated mice using a nitroxide contrast agent for EPR imaging of the redox status and a gadolinium contrast agent for MRI observation of blood– brain barrier function2015

    • Author(s)
      Emoto MC, Yamato M, Sato-Akaba H, Yamada K, Matsuoka Y, Fujii HG.
    • Journal Title

      Free Radic Res

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis2014

    • Author(s)
      Tun X, Yasukawa K, Yamada K
    • Journal Title

      Free Radic Biol Med

      Volume: 74 Pages: 108-117

    • DOI

      10.1016/j.freeradbiomed.2014.06.020

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of myeloperoxidase- and neutrophil-mediated oxidant production by tetraethyl and tetramethyl nitroxides2014

    • Author(s)
      Kajer TB, Fairfull-Smith KE, Yamasaki T, Yamada KI, Fu S, Bottle SE, Hawkins CL, Davies MJ.
    • Journal Title

      Free Radic Biol Med

      Volume: 70 Pages: 96-105

    • DOI

      10.1016/j.freeradbiomed.2014.02.011

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tempol intake improves inflammatory status in aged mice2014

    • Author(s)
      Yamato M, Ishimatsu A, Yamanaka Y, Mine T, Yamada K.
    • Journal Title

      Journal of Clinical Biochemistry and Nutrition

      Volume: 55 Issue: 1 Pages: 11-14

    • DOI

      10.3164/jcbn.14-4

    • NAID

      130004466767

    • ISSN
      0912-0009, 1880-5086
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Redox Potential of Nitroxide is an Index to Evaluate Superoxide Dismutase Mimic Activity2013

    • Author(s)
      Yamasaki T, et al.,
    • Journal Title

      Asian J Org Chem

      Volume: (in pres) Issue: 5 Pages: 388-391

    • DOI

      10.1002/ajoc.201300011

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel ascorbic acid-resistive nitroxide in a lipid emulsion: An efficient brain imaging contrast agent for MRI of small rodents.2013

    • Author(s)
      Emoto MC, Yamada KI, Yamato M, Fujii HG.
    • Journal Title

      Neurosci Lett.

      Volume: 546 Pages: 11-15

    • DOI

      10.1016/j.neulet.2013.04.044

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 脂質ラジカルをターゲットとした機能性造影剤の開発2015

    • Author(s)
      山田健一
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      神戸学院大学
    • Year and Date
      2015-03-25 – 2015-03-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] DEVELOPMENT AND UTILIZATION OF PROFLUORESCENT NITROXIDE2014

    • Author(s)
      Yamada K, Yamasaki T
    • Organizer
      International Conference SPIN 2014
    • Place of Presentation
      Zelenogradsk, Russia
    • Year and Date
      2014-09-14 – 2014-09-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] 多様な酸化還元電位を有する ニトロキシドの開発とその応用2014

    • Author(s)
      山田健一
    • Organizer
      第18回ESRフォーラム研究会
    • Place of Presentation
      名古屋大学シンポジオン会議室
    • Year and Date
      2014-07-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 脂質ラジカル蛍光プローブの 設計と疾患モデルへの応用2014

    • Author(s)
      山田健一
    • Organizer
      平成26年度 熊本大学イメージングセミナ
    • Place of Presentation
      熊本大学薬学部宮本記念館
    • Year and Date
      2014-04-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] 脂質ラジカル検出蛍光プローブの開発と応用2013

    • Author(s)
      山田健一、水戸文弥、松岡悠太、井手皐月、大石健稔、山崎俊栄、大和真由実
    • Organizer
      日本ケミカルバイオロジー学会第八回年会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] 脂質ラジカルを標的とした蛍光検出プローブの開発2013

    • Author(s)
      山田健一、水戸文弥、大石健稔、井手皐月、松岡悠太、山崎俊栄、大和真由実
    • Organizer
      第66回日本酸化ストレス学会
    • Place of Presentation
      愛知
    • Related Report
      2013 Annual Research Report
  • [Presentation] 脂質ラジカル蛍光検出プローブの開発・評価2013

    • Author(s)
      山田健一、井手皐月、水戸文弥、松岡悠太、山崎俊栄、大和真由実
    • Organizer
      日本油化学会第52回年会
    • Place of Presentation
      宮城
    • Related Report
      2013 Annual Research Report
  • [Presentation] 脂質ラジカル検出のための蛍光プローブの開発と応用2013

    • Author(s)
      山田健一、水戸文弥、松岡悠太、大石健稔、井手皐月、山崎俊栄、大和真由実
    • Organizer
      第8回日本分子イメージング学会学術集会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] Fluorescence Detection Method for Lipid-derived Radical2012

    • Author(s)
      Yamada K, et al.,
    • Organizer
      16th SFRRI Biennial Meeting
    • Place of Presentation
      London, UK
    • Related Report
      2012 Annual Research Report
  • [Presentation] Development of fluorescence detection method for lipid-derived radical2012

    • Author(s)
      Yamada K, et al.,
    • Organizer
      World Congress on 01 eo Science (WCOS 2012)
    • Place of Presentation
      Nagaskai, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] 脂質ラジカル新規検出手法の開発2012

    • Author(s)
      松岡悠太, et al.,
    • Organizer
      日本ケミカルバイオロジー学会 第7回年会
    • Place of Presentation
      京都
    • Related Report
      2012 Annual Research Report
  • [Presentation] 生体内脂質ラジカル検出蛍光プローブの開発と疾患モデルへの応用2012

    • Author(s)
      大石健稔, et al.,
    • Organizer
      第29回日本薬学会九州支部大会
    • Place of Presentation
      熊本
    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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