Regulation of nerural circuit formation by prostaglandins

• Project/Area Number: 24390017
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Kumamoto University
• Principal Investigator: Sugimoto Yukihiko 熊本大学, 生命科学研究部(薬), 教授 (80243038)
• Co-Investigator(Renkei-kenkyūsha): SEGI Eri (NISHIDA Eri) 東京理科大学, 基礎工学部, 准教授 (70378628) ; FURUYASHIKI Tomoyuki 神戸大学, 医学研究科, 教授 (20362478)
• Research Collaborator: HOHJOH Hirofumi
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
• Keywords: 視床下部 / 海馬 / 性分化 / 性行動 / プロスタノイド / 神経回路 / フェロモン / 細胞骨格 / 脳の性分化 / 抗うつ応答 / シナプス伝達 / 発熱 / アルツハイマー病

Outline of Final Research Achievements

In this study, we found that prostaglandin (PG) E2 stimulates sex-specific neural circuit formation by regulating cytoskeleton in preoptic neurons. Moreover, we revealed in zebrafish that PGF2alpha activates selective neural circuit and induces male reproductive behavior by acting on particular type of olfactory receptors.

Research Products

• [Journal Article] Prostaglandin D2 elicits the reversible neurite retraction in hypothalamic cell line. (2016)
  • Author(s): Tsuchiya H, Hohjoh H, Fujiwara Y, Sugimoto Y, Koshimizu TA.
  • Journal Title: Biochem Biophys Res Commun. Volume: 470 Issue: 4 Pages: 804-810
  • DOI: 10.1016/j.bbrc.2016.01.091
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Olfactory receptor for prostaglandin F2α mediates male fish courtship behavior. (2016)
  • Author(s): Yabuki Y, Koide T, Miyasaki N, Wakisaka N, Masuda M, Ohkura M, Nakai J, Tsuge K, Tsuchiya S, Sugimoto Y, Yoshihara Y
  • Journal Title: Nat Neurosci Volume: 19
  • Peer Reviewed
• [Journal Article] プロスタノイド受容体を介した免疫・炎症応答亢進の分子機構 (2016)
  • Author(s): 告恭史郎, 杉本幸彦
  • Journal Title: 医学のあゆみ Volume: 256(5) Pages: 561-566
• [Journal Article] Roles of prostaglandin receptors in female reproduction. (2015)
  • Author(s): Sugimoto Y, Inazumi T, Tsuchiya S
  • Journal Title: J Biochem Volume: 157 Issue: 2 Pages: 73-80
  • DOI: 10.1093/jb/mvu081
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Prostaglandin E2-induced inflammation: relevance of prostaglandin E receptors. (2015)
  • Author(s): Kawahara K, Hohjoh H, Inazumi T, Tsuchiya S, Sugimoto Y
  • Journal Title: Biochim Biophys Acta Volume: 1851 Issue: 4 Pages: 414-421
  • DOI: 10.1016/j.bbalip.2014.07.008
  • Peer Reviewed / Open Access
• [Journal Article] Dermal Vγ4(+) γδ T Cells Possess a Migratory Potency to the Draining Lymph Nodes and Modulate CD8(+) T-Cell Activity through TNF-α Production. (2015)
  • Author(s): Nakamizo S, Egawa G, Tomura M, Sakai S, Tsuchiya S, Kitoh A, Honda T, Otsuka A, Nakajima S, Dainichi T, Tanizaki H, Mitsuyama M, Sugimoto Y, Kawai K, Yoshikai Y, Miyachi Y, Kabashima K.
  • Journal Title: Journal of Investigative Dermatology Volume: 135 Issue: 4 Pages: 1007-1015
  • DOI: 10.1038/jid.2014.516
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Direct Melanoma Cell Contact Induces Stromal Cell Autocrine Prostaglandin E2-EP4 Receptor Signaling that Drives Tumor Growth, Angiogenesis and Metastasis. (2015)
  • Author(s): Inada M, Takita M, Yokoyama S, Watanabe K, Tominari T, Matsumoto C, Hirata M, Maru Y, Maruyama T, Sugimoto Y, Narumiya S, Uematsu S, Akira S, Murphy G, Nagase H, Miyaura C.
  • Journal Title: J Biol Chem. Volume: 290 Issue: 50 Pages: 29781-93
  • DOI: 10.1074/jbc.m115.669481
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] プロスタグランジンによる神経機能の調節機構 (2015)
  • Author(s): 杉本幸彦
  • Journal Title: 日薬理誌 Volume: 145 Pages: 237-242
  • NAID: 130005067370
  • Peer Reviewed
• [Journal Article] 炎症反応におけるプロスタグランジンE2の役割 (2015)
  • Author(s): 岩崎亮, 北條寛典, 杉本幸彦
  • Journal Title: 細胞 Volume: 47 Pages: 266-269
• [Journal Article] 急性炎症にかかわるプロスタグランジンとその働き (2015)
  • Author(s): 杉本幸彦
  • Journal Title: 医学のあゆみ Volume: 254（12） Pages: 1091-1096
• [Journal Article] Perivascular leukocyte clusters are essential for efficient activation of effector T cells in the skin. (2014)
  • Author(s): Natsuaki Y, Egawa G, Nakamizo S, Ono S, Hanakawa S, Okada T, Kusuba N, Otsuka A, Kitoh A, Honda T, Nakajima S, Tsuchiya S, Sugimoto Y, Ishii KJ, Tsutsui H, Yagita H, Iwakura Y, Kubo M, Ng Lg, Hashimoto T, Fuentes J, Guttman-Yassky E, Miyachi Y, Kabashima K
  • Journal Title: Nature Immunology Volume: 15 Issue: 11 Pages: 1064-9
  • DOI: 10.1038/ni.2992
  • Peer Reviewed
• [Journal Article] Prostanoid receptors and acute inflammation in skin. (2014)
  • Author(s): Hohjoh H, Inazumi T, Tsuchiya S, Sugimoto Y
  • Journal Title: Biochimie Volume: 107 Pages: 78-81
  • DOI: 10.1016/j.biochi.2014.08.010
  • Peer Reviewed
• [Journal Article] Eosinophils control the resolution of inflammation and draining lymph node hypertrophy through the proresolving mediators and CXCL13 pathway in mice (2014)
  • Author(s): Tani Y, Arita M, Isobe Y, Imoto Y, Segi-Nishida E, Sugimoto Y, Arai H
  • Journal Title: FASEB J Volume: 28 Issue: 9 Pages: 4036
  • DOI: 10.1096/fj.14-251132
  • Peer Reviewed / Open Access
• [Journal Article] Cooperative therapeutic action of retinoic acid receptor and retinoid X receptor agonists in a mouse model of Alzheimer’s disease. (2014)
  • Author(s): Kawahara K, Suenobu M, Ohtsuka H, Kuniyasu A, Sugimoto Y, Nakagomi M, Fukasawa H, Shudo K, Nakayama H
  • Journal Title: J Alzheimer’s Dis Volume: 42 Issue: 2 Pages: 587-605
  • DOI: 10.3233/jad-132720
  • Peer Reviewed / Open Access
• [Journal Article] 12-hydroxyheptadecatrienoic acid promotes epidermal wound healing by accelerating keratinocyte migration via the BLT2 receptor. (2014)
  • Author(s): Liu M, Saeki K, Matsunobu T, Okuno T, Koga T, Sugimoto Y, Yokoyama C, Nakamizo S, Kabashima K, Narumiya S, Shimizu T, Yokomizo T
  • Journal Title: J Exp Med Volume: 211 Issue: 6 Pages: 1063-1078
  • DOI: 10.1084/jem.20132063
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Birth regulates the initiation of sensory map formation through serotonin signaling. (2013)
  • Author(s): Toda T., Homma D., Tokuoka H., Hayakawa I., Sugimoto Y., Ichinose H. and Kawasaki H
  • Journal Title: Developmental Cell Volume: 27 Issue: 1 Pages: 32-46
  • DOI: 10.1016/j.devcel.2013.09.002
  • Peer Reviewed
• [Journal Article] Electroconvulsive seizure-induced changes in gene expression in the mouse hypothalamic paraventricular nucleus. (2013)
  • Author(s): Segi-Nishida, E., Sakaida, M., Sukeno, M., Imoto, Y., Kira, T., Tsuchiya, S., Sugimoto, Y., Okuno, Y.
  • Journal Title: J. Psychopharmacol. Volume: 27 Issue: 11 Pages: 1058-1069
  • DOI: 10.1177/0269881113497612
  • Peer Reviewed
• [Journal Article] Electroconvulsive seizures activate anorexigenic signals in the ventromedial nuclei of the hypothalamus. (2013)
  • Author(s): Segi-Nishida, E., Sukeno, M., Imoto, Y., Kira, T., Sakaida, M., Tsuchiya, S., Sugimoto, Y., and Okuno, Y.
  • Journal Title: Neuropharmacol. Volume: 71 Pages: 164-173
  • DOI: 10.1016/j.neuropharm.2013.03.033
  • NAID: 120005295921
  • Peer Reviewed
• [Journal Article] Intracerebral microinjection of interleukin-4/interleukin-13 reduces beta-amyloid accumulation in the ipsilateral side and improves cognitive deficits in young APP23 mice (2012)
  • Author(s): Kawahara K, Suenobu M, Yoshida A, Koga K, Hyodo A, Ohtsuka H, Kuniyasu A, Tamamaki N, Sugimoto Y, Nakayama H
  • Journal Title: Neuroscience Volume: 207 Pages: 243-260
  • DOI: 10.1016/j.neuroscience.2012.01.049
  • Peer Reviewed
• [Journal Article] Improvement of cognitive function in Alzheimer's disease model mice by genetic and pharmacological inhibition of the EP(4) receptor (2012)
  • Author(s): Hoshino T, Namba T, Takehara M, Murao N, Matsushima T, Sugimoto Y, Narumiya S, Suzuki T, Mizushima T
  • Journal Title: J.Neurochem. Volume: 120 Issue: 5 Pages: 795-805
  • DOI: 10.1111/j.1471-4159.2011.07567.x
  • Peer Reviewed
• [Journal Article] Restriction of Mast Cell Proliferation through Hyaluronan Synthesis by Co-cultured Fibroblasts (2012)
  • Author(s): Takano H, Furuta K, Yamashita K, Sakanaka M, Itano N, Gohda E, Nakayama K, Kimata K, Sugimoto Y, Ichikawa A., Tanaka S.
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 35 Issue: 3 Pages: 408-412
  • DOI: 10.1248/bpb.35.408
  • NAID: 130001872324
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed
• [Journal Article] The intrinsic prostaglandin E(2)-EP(4) system of the renal tubular epithelium limits the development of tubulointerstitial fibrosis in mice (2012)
  • Author(s): Nakagawa N, Yuhki KI, Kawabe JI, Fujino T, Takahata O, 他9名
  • Journal Title: Kidney Int Volume: (in press) Issue: 2 Pages: 158-171
  • DOI: 10.1038/ki.2012.115
  • Peer Reviewed
• [Journal Article] Inhibition of EP4 signaling attelluates aortic aneurysm formation (2012)
  • Author(s): Yokoyama U
  • Journal Title: PLoS One Volume: 7 Issue: 5 Pages: e36724-e36724
  • DOI: 10.1371/journal.pone.0036724
  • Peer Reviewed
• [Presentation] 旧くて新しいプロスタグランジンの生理機能 (2016)
  • Author(s): 杉本幸彦
  • Organizer: 第165回東京脂質談話会
  • Place of Presentation: 東京
  • Year and Date: 2016-01-13
  • Invited
• [Presentation] 初期発生におけるプロスタグランジン受容体の新規機能:モデル生物としてゼブラフィッシュを用いた解析から (2015)
  • Author(s): 土屋創健, 岩崎亮, 告恭史郎, 杉本幸彦
  • Organizer: 第88回日本生化学会大会（BMB2015）
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] ゼブラフィッシュ初期発生におけるプロスタグランジンI2受容体の機能探索 (2015)
  • Author(s): 岸本幸一朗, 告恭史郎, 岩崎亮, 川原敦雄, 稲住知明, 土屋創健, 杉本幸彦
  • Organizer: 第32回日本薬学会九州支部大会
  • Place of Presentation: 延岡
  • Year and Date: 2015-11-28
• [Presentation] オス特有の脳構造構築におけるプロスタグランジンE2-EP4受容体の役割 (2015)
  • Author(s): 平川昌樹, 北條寛典, 稲住知明, 土屋創健, 杉本幸彦
  • Organizer: 第32回日本薬学会九州支部大会
  • Place of Presentation: 延岡
  • Year and Date: 2015-11-28
• [Presentation] 第一世代の脂質メディエーター・プロスタグランジンの生理病態作用 (2015)
  • Author(s): 杉本幸彦
  • Organizer: 第37回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 熊本
  • Year and Date: 2015-11-19
  • Invited
• [Presentation] ゼブラフィッシュにおけるプロスタノイド受容体の機能解析 (2015)
  • Author(s): 告恭史郎, 岩崎亮, 岸本幸一朗, 川原敦雄, 稲住知明, 土屋創健, 杉本幸彦
  • Organizer: 第14回次世代を担う若手ファーマ・バイオフォーラム2015
  • Place of Presentation: 千葉
  • Year and Date: 2015-09-12
• [Presentation] Prostaglandin E2 facilitates neurite outgrowth in preoptic neurons. (2015)
  • Author(s): Hohjoh H, Yoshioka M, Tsuchiya H, Tsuchiya S, Misumi S, Segi-Nishida E, Sugimoto Y
  • Organizer: 6th International Conference on Phospholipase A2 and Lipid Mediators (PLM2015)
  • Place of Presentation: Tokyo
  • Year and Date: 2015-02-10 – 2015-02-12
• [Presentation] プロスタグランジンE2-EP4による視索前野ニューロンの神経突起伸長制御 (2014)
  • Author(s): 北條寛典, 吉岡美樹, 土屋裕義, 土屋創健, 三隅将吾, 瀬木-西田恵里, 杉本幸彦
  • Organizer: 第8回次世代を担う若手医療薬科学シンポジウム
  • Place of Presentation: 熊本
  • Year and Date: 2014-11-15 – 2014-11-16
• [Presentation] プロスタグランジンEP4受容体による視索前野ニューロンの細胞骨格制御 (2014)
  • Author(s): 北條寛典, 吉岡美樹, 土屋裕義, 土屋創健, 川原浩一, 三隅将吾, 瀬木-西田恵里, 杉本幸彦
  • Organizer: 第56回 日本脂質生化学会
  • Place of Presentation: 大阪
  • Year and Date: 2014-06-06 – 2014-06-07
• [Presentation] 魚類における性行動発現の嗅覚メカニズム：性フェロモン・プロスタグランジンF2aが活性化するゼブラフィッシュ嗅覚受容体および神経回路素子の同定 (2013)
  • Author(s): 矢吹陽一、小出哲也、宮坂信彦、脇阪紀子、増田美和、告恭史郎、杉本幸彦、渡邉和忠、吉原良浩
  • Organizer: Neuro2013 第36回日本神経科学大会、第56回日本神経化学会大会、第23回日本神経回路学会大会
  • Place of Presentation: 京都
• [Presentation] プロスタグランジンによる炎症惹起の分子機構 (2013)
  • Author(s): 杉本幸彦
  • Organizer: 生体機能と創薬シンポジウム2013
  • Place of Presentation: 福岡
  • Invited
• [Presentation] プロスタグランジン受容体との相互作用因子の探索 (2013)
  • Author(s): 島津佑太朗, 森本和志, 土屋創健, 杉本幸彦
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] プロスタグランディンと雌性生殖生理 (2013)
  • Author(s): 杉本幸彦
  • Organizer: フォーラム2013：衛生薬学・環境トキシコロジー
  • Place of Presentation: 福岡
  • Invited
• [Presentation] プロスタグランジンEP4受容体による視索前野ニューロンの細胞骨格制御 (2013)
  • Author(s): 北條寛典、吉岡美樹、土屋裕義、土屋創健、川原浩一、三隅将吾、瀬木-西田恵里、杉本幸彦
  • Organizer: 第12回次世代を担う若手ファーマ・バイオフォーラム2013
  • Place of Presentation: 福岡
• [Presentation] プロスタグランジンEP4受容体による視索前野ニューロンの細胞骨格制御 (2012)
  • Author(s): 北條寛典、吉岡美樹、土屋裕義、土屋創健、三隅将吾、瀬木-西田恵里、杉本幸彦
  • Organizer: 第65回日本薬理学会西南部会
  • Place of Presentation: 熊本大学(熊本)
  • Year and Date: 2012-11-23
• [Presentation] プロスタグランジンEP4受容体による視索前野ニューロンの細胞骨格制御 (2012)
  • Author(s): 北條寛典、吉岡美樹、土屋裕義、土屋創健、三隅将吾、瀬木-西田恵里、杉本幸彦
  • Organizer: 第28回日本薬学会九州支部大会
  • Place of Presentation: 熊本大学(熊本)
• [Presentation] ミクログリアのIL-33遺伝子発現に対するプロスタグランジンE2の効果 (2012)
  • Author(s): 鈴木佑治、稲住知明、土屋創健、川原浩一、杉本幸彦
  • Organizer: 第28回日本薬学会九州支部大会
  • Place of Presentation: 熊本大学(熊本)
• [Book] 疾患モデルの作製と利用：脂質代謝異常と関連疾患・下巻（尾池雄一, 佐々木雄彦, 村上誠, 矢作直也編） (2015)
  • Author(s): 北條寛典, 土屋創健, 杉本幸彦
  • Total Pages: 13
  • Publisher: エル・アイ・シー
• [Book] Bioactive Lipid Mediators, Current Reviews and Protocols (2015)
  • Author(s): Inazumi T, Sugimoto Y
  • Total Pages: 10
  • Publisher: Springer