Role of glycans in chronic inflammation revealed by novel anti-carbohydrate antibodies
| Project/Area Number |
24390018
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Chiba University (2015)
Hoshi University (2014)
University of Shizuoka (2012-2013) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IMAI Yasuyuki 静岡県立大学, 薬学部, 教授 (80160034)
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| Co-Investigator(Renkei-kenkyūsha) |
KOBAYASHI Motohiro 福井大学, 医学部, 教授 (00362137)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Keywords | リンパ球ホーミング / 抗糖鎖抗体 / シアリルルイスX / L-セレクチン / P-セレクチン / E-セレクチン / 糖鎖 / 炎症 |
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| Outline of Final Research Achievements |
In this study, we succeeded in the generation of novel monoclonal antibodies reactive with a fucosylated glycan epitope sialyl Lewis X based on a newly developed method. In mouse contact hypersensitivity model, both F1 and F2 significantly inhibited ear swelling by blocking sialyl Lewis X-dependent leukocyte infiltration upon antigen challenge in sensitized animals. In silicosis model, these monoclonal antibodies inhibited lung fibrosis at the chronic stage by blocking sialyl Lewis X-dependent acute leukocyte infiltration after administration of silica. Taken together, these results indicate that functional blockage of sialyl Lewis X at particular stage of inflammation leads to efficient control over inflammatory disorders.
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Report
(5 results)
Research Products
(49 results)
