| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Outline of Final Research Achievements |
Ethynylbenzene-substituted glycol, as a versatile crick-reaction probe, was synthesized and their RNA post-labeling property was investigated. Stereoselective synthesis of 1-deoxy-1-ethynyl-β-D-ribofuranose was performed for RNA labeling. SiRNAs and miRNAs bearing urea/thioures-bridged aromatic compounds at their 3'-end were also prepared and these dsRNAs definitely showed nuclease resistance. Oligonucleotides with glucosamine at the 3'-position were synthesized reaction and their biological activity was investigated. Some of chemically modified siRNAs at the 3’-position showed avoidance of off-target effect.
Chemically modified synthetic miRNA-205 inhibited the growth of melanoma cells in vitro and in vivo. A clinical trial of miR-205 against canine melanoma is now in progress; thus far, intratumoral injection of miR-205 into a recurrent tumor derived from naturally occurring canine melanoma has resulted in complete remission in 2 of 5 cases.
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