Owing to the development of RNA medicine, discovery of practical RNA molecules and synthesis of nucleic acid PET probes
Project/Area Number |
24390025
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Drug development chemistry
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Research Institution | Gifu University |
Principal Investigator |
KITADE Yukio 岐阜大学, 工学部, 教授 (20137061)
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Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
|
Keywords | RNA創薬 / オリゴヌクレオチド / クリック反応 / ラベル標識化 / 薬物動態 / PETプローブ / バイオイメージング / ドラッグデリバリー / RNA創薬 / ラベル標識 / PETプローブ |
Outline of Final Research Achievements |
Ethynylbenzene-substituted glycol, as a versatile crick-reaction probe, was synthesized and their RNA post-labeling property was investigated. Stereoselective synthesis of 1-deoxy-1-ethynyl-β-D-ribofuranose was performed for RNA labeling. SiRNAs and miRNAs bearing urea/thioures-bridged aromatic compounds at their 3'-end were also prepared and these dsRNAs definitely showed nuclease resistance. Oligonucleotides with glucosamine at the 3'-position were synthesized reaction and their biological activity was investigated. Some of chemically modified siRNAs at the 3’-position showed avoidance of off-target effect. Chemically modified synthetic miRNA-205 inhibited the growth of melanoma cells in vitro and in vivo. A clinical trial of miR-205 against canine melanoma is now in progress; thus far, intratumoral injection of miR-205 into a recurrent tumor derived from naturally occurring canine melanoma has resulted in complete remission in 2 of 5 cases.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Chemically Modified Synthetic microRNA-205 Inhibits the Growth of Melanoma Cells In Vitro and In Vivo.2013
Author(s)
Noguchi, S., Iwasaki, J., Kumazaki, M., Mori, T., Maruo, K., Saka,i H., Yamada, N., Shimada, K., Naoe, T., Kitade, Y., and Akao, Y.
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Journal Title
Mol. Ther.
Volume: 21
Issue: 6
Pages: 1204-1211
DOI
Related Report
Peer Reviewed
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