Development of synthetic platoform of proteins
| Project/Area Number |
24390026
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Drug development chemistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
OTAKA Akira 徳島大学, ヘルスバイオサイエンス研究部, 教授 (20201973)
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| Co-Investigator(Kenkyū-buntansha) |
SHIGENAGA Akira 徳島大学, 大学院ヘルスバイオサイエンス研究部, 講師 (10423394)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Keywords | タンパク質 / タンパク質化学合成 / チオエステル / Native Chemical Ligation / ペプチド合成 / NCL / 速度論的反応 / アシル転移 |
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| Outline of Final Research Achievements |
We have developed N-sulfanylethylanilide (SEAlide) peptides. Extensive examination of SEAlide peptides showed that the amide-type SEAlide peptides can be directly and efficiently involved in NCL via thioester species in the presence of phosphate salts. The presence or absence of phosphate salts provided kinetically controllable chemoselectivity in NCL for SEAlide peptides. This allowed SEAlide peptides to be used in both one-pot/N-to-C-directed sequential NCL under kinetically controlled conditions, and the convergent coupling of large peptide fragments, which facilitated the chemical synthesis of proteins over about 100 residues. The use of SEAlide peptides, enabling sequential NCL operated under kinetically controlled conditions, and the convergent coupling, were used for the total chemical synthesis of a 162-residue monoglycosylated GM2-activator protein (GM2AP) analog.
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Report
(4 results)
Research Products
(65 results)
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[Journal Article] Development of a fluoride-responsive amide bond cleavage device that is potentially applicable to a traceable linker2014
Author(s)
J. Yamamoto, N. Maeda, C. Komiya, T. Tanaka, M. Denda, K. Ebisuno, W. Nomura, H. Tamamura, Y. Sato, A. Yamauchi, A. Shigenaga, and A. Otaka
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Journal Title
Tetrahedron
Volume: 70
Issue: 34
Pages: 5122-5127
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Development of a traceable linker containing a thiol-responsive amino acid for the enrichment and selective labelling of target proteins2014
Author(s)
J. Yamamoto, M. Denda, N. Maeda, M. Kita, C. Komiya, T. Tanaka, W. Nomura, H. Tamamura, Y. Sato, A. Yamauchi, A. Shigenaga, and A. Otaka
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Journal Title
Org. Biomol. Chem.
Volume: 12
Issue: 23
Pages: 3821-3826
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] M6P修飾型GM2APの合成検討2015
Author(s)
成瀬 公人, 佐藤 浩平, 坂本 健, 猪熊 翼, 重永 章, 大髙 章
Organizer
日本薬学会第135年会
Place of Presentation
神戸学院大学（兵庫県神戸市）
Year and Date
2015-03-25 – 2015-03-28
Related Report
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[Presentation] ペプチドチオエステル調製のためのクマリン型補助基の開発2014
Author(s)
江藤 三弘, 傳田 将也, 佐藤 浩平, 坂本 健, 猪熊 翼, 重永 章, 大髙 章
Organizer
第53回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
Place of Presentation
広島国際会議場（広島県広島市）
Year and Date
2014-11-08 – 2014-11-09
Related Report
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[Presentation] 新規タンパク質チオエステル調製法の開発2014
Author(s)
津田 雄介, 重永 章, 傳田 将也, 佐藤 浩平, 北風 圭介, 中村 太寛, 猪熊 翼, 伊藤 孝司, 大髙 章
Organizer
第53回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
Place of Presentation
広島国際会議場（広島県広島市）
Year and Date
2014-11-08 – 2014-11-09
Related Report
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[Presentation] 過酸化水素応答型ペプチド結合切断デバイスの開発研究2014
Author(s)
北 未来, 山本 純, 戎野 紘司, 小宮 千明, 宮本 理人, 土屋 浩一郎, 重永 章, 大髙 章
Organizer
日本ケミカルバイオロジー学会第9回年会
Place of Presentation
大阪大学（大阪府豊中市）
Year and Date
2014-06-11 – 2014-06-13
Related Report
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