Integrated analysis of transcriptome and metabolome in Japanese medicinal plants

• Project/Area Number: 24390030
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Environmental pharmacy
• Research Institution: Chiba University
• Principal Investigator: SAITO Kazuki 千葉大学, 薬学研究科(研究院), 教授 (00146705)
• Co-Investigator(Kenkyū-buntansha): YAMAZAKI Mami 千葉大学, 大学院薬学研究院, 准教授 (70222370) ; YOSHIMOTO Naoko 千葉大学, 大学院薬学研究院, 助教 (10415333) ; SUZUKI Hideyuji 公益財団法人かずさDNA研究所, バイオ研究開発部, 主席研究員 (80276162)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2014: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2013: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
• Keywords: 薬用資源 / トランスクリプトーム / メタボローム / 二次代謝物 / 二次代謝 / 二次代謝産物

Outline of Final Research Achievements

We have conducted the deep transcriptome and metabolite analyses on the representative medicinal plants grown in Japan. By the transcriptomics analyses with a next-generation DNA sequencer, we have successfully assigned a number of genes presumably involved in the biosynthesis of pharmacologically-active ingredients in the Leguminous medicinal plants, Sophora flavescens and Pueraria lobata.

Research Products

• [Journal Article] Transcriptome analysis of 9 tissues to discover genes involved in the biosynthesis of active ingredients in Sophora flavescens. (2015)
  • Author(s): Rongchun Han, Hiroki Takahashi, Michimi Nakamura, Bunsupa Somnuk, Naoko Yoshimoto, Hirobumi Yamamoto, Hideyuki Suzuki, Daisuke Shibata, Mami Yamazaki and Kazuki Saito
  • Journal Title: Biol. Pharm. Bull. Volume: 印刷中
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] RNA-Seq analysis on 9 tissues of Sophora flavescens reveals putative genes involved in the biosynthesis of quinolizidine alkaloids and isoflavonoids, (2014)
  • Author(s): 1.韓栄春，中村道美，Bunsupa Somnuk，山本浩文，鈴木秀幸，高橋弘喜，吉本尚子，山崎真巳，斉藤和季
  • Organizer: 日本生薬学会第61回年会
  • Place of Presentation: 福岡大学(福岡)
  • Year and Date: 2014-09-13 – 2014-09-14
• [Presentation] RNA-Seq Analysis on Pueraria lobata Ohwi (2014)
  • Author(s): 韓 栄春、高橋 弘喜、中村 道美、吉本 尚子、鈴木 秀幸、 柴田 大輔、山崎 真巳、斉藤和季
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本
• [Presentation] Discovery of novel genes in plant secondary metabolism by integrated omics approach (2012)
  • Author(s): Kazuki Saito
  • Organizer: The 43^<rd> Annual Meeting of the Korean Society of Pharmacognosy
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2012-12-06
• [Remarks] 千葉大学大学院薬学研究院遺伝子資源応用研究室
  • URL: http://www.p.chiba-u.jp/lab/idenshi/index.html/
• [Remarks] 千葉大学大学院薬学研究院遺伝子資源応用研究室
  • URL: http://www.p.chiba-u.ac.jp/lab/idenshi/index.html/
• [Remarks]
  • URL: http://www.p.chiba-u.ac.jp/lab/idenshi/index.html/